Pores formation on cell membranes by hederacolchiside A1 leads to a rapid release of proteins for cytosolic subproteome analysis

Mazzucchelli, Gabriel; Cellier, Nicolas A; Mshviladzade, Vakhtang; Elias, Riad; Shim, Yong-Ho; Touboul, David; Quinton, Loïc; Brunelle, Alain; Laprevote, Olivier; De Pauw, Edwin; De Pauw-Gillet, Marie-Claire

doi:10.1021/pr7006973

Article (Scientific journals)

Pores formation on cell membranes by hederacolchiside A1 leads to a rapid release of proteins for cytosolic subproteome analysis

Mazzucchelli, Gabriel; Cellier, Nicolas A; Mshviladzade, Vakhtang et al.

2008 • In Journal of Proteome Research, 7 (4), p. 1683-1692

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/6696

DOI
10.1021/pr7006973

PubMed
18338859

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Keywords :

saponin; CMC; pores; mel-5; 2D gels; 2D-LC-MS/MS; TOF-SIMS; soluble biomarkers; fractionation

Abstract :

[en] Hederacolchiside A1 was used to progressively permeabilize the membrane of human melanoma MEL-5 cells. Holes formation was followed by Scanning Electron Microscopy and interaction of the saponin with cholesterol and phospholipids by TOF-SIMS. 2D-LC-MS/MS and 2D-SDS-PAGE show that the release of soluble proteins into serum-free culture media increases with time. This can lead to a new rapid and efficient strategy to analyze the cytosolic subproteome and it opens the door to get information from the cytosolic compartment for clinical proteomic studies.

Research Center/Unit :

Giga-Systems Biology and Chemical Biology - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Mazzucchelli, Gabriel ; Université de Liège - ULiège > Centre interfac. d'analyse des résidus en traces (CART)

Cellier, Nicolas A

Mshviladzade, Vakhtang

Elias, Riad

Shim, Yong-Ho

Touboul, David; Institut de Chimie des Substances Naturelles > Laboratoire de Spectrométrie de Masse

Quinton, Loïc ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

Brunelle, Alain; Institut de Chimie des Substances Naturelles > Laboratoire de Spectrométrie de Masse

Laprevote, Olivier; Institut de Chimie des Substances Naturelles > Laboratoire de Spectrométrie de Masse

De Pauw, Edwin ; Université de Liège - ULiège > Département de chimie (sciences) > GIGA-R : Laboratoire de spectrométrie de masse (L.S.M.)

De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques

Language :

English

Title :

Pores formation on cell membranes by hederacolchiside A1 leads to a rapid release of proteins for cytosolic subproteome analysis

Publication date :

2008

Journal title :

Journal of Proteome Research

ISSN :

1535-3893

eISSN :

1535-3907

Publisher :

American Chemical Society, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

1683-1692

Peer reviewed :

Peer Reviewed verified by ORBi

Name of the research project :

• Projet Intass –Georgie-0491 (1999 –2001 G. Balansard, coordinateur) : étude de saponines.

Funders :

FRFC - Fonds de la Recherche Fondamentale Collective

Available on ORBi :

since 20 February 2009

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