Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus

Perilli, Mariagrazia; Franceschini, Nicola; Segatore, Bernardetta; Amicosante, Gianfranco; Oratore, Arduino; Duez, Colette; Joris, Bernard; Frère, Jean-Marie

doi:10.1111/j.1574-6968.1991.tb04393.x

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Article (Scientific journals)

Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus

Perilli, Mariagrazia; Franceschini, Nicola; Segatore, Bernardetta et al.

1991 • In FEMS Microbiology Letters, 83, p. 79-84

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/66949

DOI
10.1111/j.1574-6968.1991.tb04393.x

PubMed
1778425

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Keywords :

beta-lactamase; Citrobacter diversus

Abstract :

[en] The gene coding for the class A beta-lactamase of Citrobacter diversus has been cloned and sequenced. It contains the information for a 294-amino-acid precursor protein, including a 27-residue N-terminal signal peptide. The deduced sequence of the N-terminal portion of the mature protein is in excellent agreement with that determined by microsequencing of the protein and readily explains the pI differences observed between the naturally occurring forms I and II of the enzyme. The sequence of the mature protein exhibits a very high degree of similarity with that of the Klebsiella oxytoca class A beta-lactamase

Disciplines :

Microbiology

Author, co-author :

Perilli, Mariagrazia; University of L'Aquila, Italy

Franceschini, Nicola; University of L'Aquila, Italy

Segatore, Bernardetta; University of L'Aquila, Italy

Amicosante, Gianfranco; University of L'Aquila, Italy

Oratore, Arduino; University of L'Aquila, Italy

Duez, Colette ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Joris, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines

Frère, Jean-Marie ; Université de Liège - ULiège > Centre d'ingénierie des protéines

Language :

English

Title :

Cloning and nucleotide sequencing of the gene encoding the beta-lactamase from Citrobacter diversus

Publication date :

1991

Journal title :

FEMS Microbiology Letters

ISSN :

0378-1097

eISSN :

1574-6968

Publisher :

Blackwell Publishing, Oxford, United Kingdom

Volume :

Pages :

79-84

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 July 2010

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Bibliography

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