Article (Scientific journals)
The surprising diversity of clostridial hydrogenases: a comparative genomic perspective
Calusinska, Magdalena; Happe, Thomas; Joris, Bernard et al.
2010In Microbiology, 156, p. 1575-1588
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Keywords :
hydrogenases; Clostridium; genomes
Abstract :
[en] Among the large variety of micro-organisms capable of fermentative hydrogen production, strict anaerobes such as members of the genus Clostridium are the most widely studied. They can produce hydrogen by a reversible reduction of protons accumulated during fermentation to dihydrogen, a reaction which is catalysed by hydrogenases. Sequenced genomes provide completely new insights into the diversity of clostridial hydrogenases. Building on previous reports, we found that [FeFe] hydrogenases are not a homogeneous group of enzymes, but exist in multiple forms with different modular structures and are especially abundant in members of the genus Clostridium. This unusual diversity seems to support the central role of hydrogenases in cell metabolism. In particular, the presence of multiple putative operons encoding multisubunit [FeFe] hydrogenases highlights the fact that hydrogen metabolism is very complex in this genus. In contrast with [FeFe] hydrogenases, their [NiFe] hydrogenase counterparts, widely represented in other bacteria and archaea, are found in only a few clostridial species. Surprisingly, a heteromultimeric Ech hydrogenase, known to be an energy-converting [NiFe] hydrogenase and previously described only in methanogenic archaea and some sulfur-reducing bacteria, was found to be encoded by the genomes of four cellulolytic strains: Clostridum cellulolyticum, Clostridum papyrosolvens, Clostridum thermocellum and Clostridum phytofermentans
Research center :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Microbiology
Author, co-author :
Calusinska, Magdalena ;  Université de Liège - ULiège > Centre d'ingénierie des protéines
Happe, Thomas
Joris, Bernard ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes - Centre d'ingénierie des protéines
Wilmotte, Annick  ;  Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Language :
English
Title :
The surprising diversity of clostridial hydrogenases: a comparative genomic perspective
Alternative titles :
[fr] La diversité surprenante des hydrogénases de clostridies: une comparaison génomique
Publication date :
2010
Journal title :
Microbiology
ISSN :
1350-0872
eISSN :
1465-2080
Publisher :
Society for General Microbiology, Reading, United Kingdom
Volume :
156
Pages :
1575-1588
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
ARC project Micro-H2
Funders :
Communauté française de Belgique - CfB, projet ARC
Available on ORBi :
since 25 June 2010

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