[en] Being able to participate in the reverse cholesterol transport (RCT), high density lipoproteins (HDL) are known to be anti-atherogenic. In order to understand such a process, it is thus essential to have a detailed knowledge of the structure and molecular organisation of HDL.
Reconstituted nascent high density lipoproteins (r-HDL), consisting of synthetic phospholipids together with different apolipoproteins (apo A-I, A-IV and E), were thus analysed by scanning tunnelling microscopy (STM).
Both shape and dimensions of the discoidal HDL particles measured by this technique were found in good agreement with the data available from the literature.
The accuracy of the STM pictures presented in this paper enables for the first time the visualisation of the molecular organisation of such macromolecules. The arrangement of the protein as antiparallel helical segments, is consistent with the general mode of organisation of apolipoprotein/phospholipid discoidal particles previously reported.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Culot, C.
Durant, F.
Lazarescu, S.
Thiry, P. A.
Vanloo, B.
Rosseneu, M. Y.
Lins, Laurence ; Université de Liège - ULiège > Chimie et bio-industries > Centre de Bio. Fond. - Section de Biologie moléc. et numér.
Brasseur, R.
Language :
English
Title :
Structural investigation of reconstituted high density lipoproteins by scanning tunnelling microscopy
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Boguski M.S., Freeman M., Elshourbagy N.A., Taylor J.M., Gordon J.I. J. Lipid Res. 27:1986;1011.
Lins L., Brasseur R., De Pauw M., van Biervliet J.P., Ruysschaert J.M., Rosseneu M., Vanloo B. Biochim. Biophys. Acta. 1258:1995;10.
A. Jonas, A structure and function of apolipoproteins, in: M. Rosseneu (Ed.), CRC Press, Boca, Raton, USA, 1992, pp. 217-250.
Brasseur R., De Meutter J., Vanloo B., Goormaghtigh E., Ruysschaert J.M., Rosseneu M. Biochim. Biophys. Acta. 1043:1990;245.
Brasseur R., Lins L., Vanloo B., Ruysschaert J.M., Rosseneu M. Proteins. 13:1992;246.
Segrest J.P., Jackson R.L., Morrisett J.D., Gotto A.M. FEBS Lett. 38:1974;247.
Wald J.H., Goormaghtigh E., De Meutter J., Ruysschaert J.M., Jonas A. J. Biol. Chem. 265:1990;20044.
Lins L., Brasseur R., Rosseneu M., Vanloo B., Ruysschaert J.M. Biochim. Biophys. Acta. 1149:1993;267.
Marcel Y.L., Provost P.R., Koa H., Raffai E., Dac N.V., Fruchart J.C., Rassart E. J. Biol. Chem. 266:1991;3644.
Lins L., Piron S., Conrath K., Vanloo B., Brasseur R., Rosseneu M., Baert J., Ruysschaert J.M. Biochim. Biophys. Acta. 1151:1993;137.
Emmanuel F., Steinmetz F., Rosseneu M., Brasseur R., Gosselet N., Attenot F., Cuin S., Seguret S., Latta M., Fruchart J.C., Denèfle P. J. Biol. Chem. 269:1994;29883.
M. Rosseneu, B. Vanloo, L. Lins, J. Corijn, J.P. van Bierliet, J.M. Ruysschaert, R. Brasseur, High density lipoproteins and atherosclerosis, in: N.E. Miller, A.R. Tall (Eds.), Elsevier, Amsterdam, 1992, pp.105-114.
Sparks D.L., Philips M.C., Lund-Katz S. J. Biol. Chem. 267:1992;25830.
Sparks D.L., Philips M.C., Lund-Katz S. J. Biol. Chem. 267:1992;25839.
De Pauw M., Vanloo B., Weisgraber K., Rosseneu M. Biochemistry. 34:1995;10953.
Matz C.E., Jonas A. J. Biol. Chem. 257:1982;4535.
Roberts C.J., Davies M.C., Tendler S.J.B., Williams P.M., Davies J., Dawkes A.C., Yearwood G.D.L., Edwards J.C. Ultramicroscopy. 62:1996;149.
Wilkins M.J., Davies M.C., Jackson D.E., Roberts C.J., Tendler S.J.B., Williams P.M. Appl. Phys. Lett. 60:1992;1436.
Guckenberg R., Heim M., Cevc G., Knapp H.F., Wiegräbe W., Hilleband A. Science. 266:1994;1538.
Fan F.R., Bard A.J. Science. 270:1995;1849.
Rahman M., Brasseur R. J. Mol. Graph. 12:1994;212.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.