Article (Scientific journals)
Stimulation of the lipoxygenase pathway is associated with systemic resistance induced in bean by a nonpathogenic Pseudomonas strain
Ongena, MARC; Duby, Franceline; Rossignol, Fanny et al.
2004In Molecular Plant-Microbe Interactions, 17 (9), p. 1009-1018
Peer Reviewed verified by ORBi
 

Files


Full Text
MPMI 2004.pdf
Publisher postprint (291.62 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
plant protection
Abstract :
[en] Systemic defense reactions induced in bean by the nonpathogenic Pseudomonas putida BTP1 strain reduced disease caused by Botrytis cinerea. Phenylalanine ammonialyase activity and the level of endogenous free sallicylic acid were compared in plant growth-promoting rhizobacteria-treated versus control plants, but no significant differences were detected. Furthermore, no enhanced fungitoxicity was detected in methanolic leaf extracts, suggesting that accumulation of bean phytoalexins was not part of the stimulated defense mechanisms. However, BTP1-inoculated plants showed increased levels of both linoleic and linolenic acids. On this basis, we further investigated whether the lipoxygenase pathway, leading to antifungal phytooxylipins, could have been stimulated. Two key enzymatic activities of this metabolic route, namely lipoxygenase and hydroperoxidelyase, were significantly stimulated during the first four days after challenging BTP1-treated plants with the pathogen. This was observed in parallel with a more rapid consumption of the respective substrates of these enzymes, as revealed by measurements of endogenous concentrations of linolenic acid and their hydroperoxide derivatives. Moreover, headspace-gas chromatography analyses showed significantly higher concentrations of the fungitoxic final product Z-3-hexenal in leaves from BTP1-inoculated beans as compared with control plants. Taken together, these results strongly suggest that the oxylipin pathway can be associated with enhanced disease resistance induced in bean plants by nonpathogenic rhizobacteria.
Disciplines :
Biochemistry, biophysics & molecular biology
Microbiology
Biotechnology
Author, co-author :
Ongena, MARC ;  Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech
Duby, Franceline ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Rossignol, Fanny
Fauconnier, Marie-Laure  ;  Université de Liège - ULiège > Sciences agronomiques > Biologie végétale
Dommes, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Biologie moléculaire et biotechnologie végétales
Thonart, Philippe ;  Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
Language :
English
Title :
Stimulation of the lipoxygenase pathway is associated with systemic resistance induced in bean by a nonpathogenic Pseudomonas strain
Publication date :
September 2004
Journal title :
Molecular Plant-Microbe Interactions
ISSN :
0894-0282
eISSN :
1943-7706
Publisher :
Amer Phytopathological Soc, St Paul, France
Volume :
17
Issue :
9
Pages :
1009-1018
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 February 2009

Statistics


Number of views
143 (62 by ULiège)
Number of downloads
9 (8 by ULiège)

Scopus citations®
 
131
Scopus citations®
without self-citations
124
OpenCitations
 
102

Bibliography


Similar publications



Contact ORBi