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EFFECTS OF O2 TENSION AND GLUCOSE CONCENTRATION ON THE CELLULAR RESPIRATION OF EQUINE ARTICULAR CHONDROCYTES IN CULTURE.
Schneider, Nicole; Lejeune, Jean-Philippe; Duyckaerts, Claire et al.
2003Free Radical Research
 

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Keywords :
horse; equine; chondrocytes; cellular respiration; glucose; oxygen; joint
Abstract :
[en] In vivo, articular chondrocytes are exposed to 5 to 10% O2. Chondrocytes are also suspected to produce reactive oxygen species when submitted to anoxia/re-oxygenation. We were interested to study the effects of O2 and glucose variations on cellular respiration, glucose consumption and lactate production. Equine articular chondrocytes were cultured in suspension for 2 days under 5 or 21 % O2 in the gaseous phase, and with 0, 1.0 or 4.5 g/L glucose. The viable cells were then counted and the respiration rate (O2 consumption) of 10.106 cells was monitored by oxymetry for 2 hours; after oxymetry, glucose and lactate were measured in the medium (enzymatic assays). After 2 days, the cell viability was the best at 5% O2 and 1g/L glucose; it decreased at 4.5 g/L glucose and was the worst at 0g/L glucose, for the two O2 tensions (n=3). There was no obvious difference of the respiration rate between cells cultured at 5 and 21% O2, but respiration of chondrocytes was surprisingly low. When cells were submitted to 20 min anoxia at 0% O2, the O2 consumption was doubled at re-oxygenation for cells previously cultured at 21% O2. Glucose and lactate values found in the medium after oxymetry: lactate release in medium was similar (36.23 and 34.57 mg/L respectively) for cells cultured with 1g glucose and 5 or 21% O2 conditions; lactate values were low (2.03 and 8,63 mg/L respectively) for 4.5 g glucose and 5 or 21% O2. Glucose uptake was not different whatever the culture conditions. These results indicate a low cellular respiration with a lactate production linked to the glucose concentrationin the medium, and raise the question of the capacity of chondrocytes to produce ROS in vivo starting from the mitochondrial chain.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Life sciences: Multidisciplinary, general & others
Veterinary medicine & animal health
Author, co-author :
Schneider, Nicole ;  Université de Liège - ULiège > CORD, ULiège et Centre Européen du Cheval de Mont-le-Soie, Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Lejeune, Jean-Philippe ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Duyckaerts, Claire ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Sluse, Francis ;  Université de Liège - ULiège > Département des sciences de la vie > Bioénergétique et physiologie cellulaire
Mouithys-Mickalad, Ange ;  Université de Liège - ULiège > Centre de l'oxygène : Recherche et développement (C.O.R.D.)
Deby-Dupont, Ginette;  Université de Liège - ULiège > CORD
Serteyn, Didier  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
EFFECTS OF O2 TENSION AND GLUCOSE CONCENTRATION ON THE CELLULAR RESPIRATION OF EQUINE ARTICULAR CHONDROCYTES IN CULTURE.
Publication date :
2003
Event name :
Free Radical Research
Event place :
Ioannina, Greece
Event date :
26-29 juin 2003
Audience :
International
Funders :
Région wallonne. Ministère de l'Agriculture, de la Nature, de la Forêt, de la Ruralité, du Tourisme, du Patrimoine [BE]
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