[en] Background: Trachylobane diterpenes are secondary metabolites quite rare in nature and their bioactivities are poorly known. Recently we have described the cytotoxic activity of ent- trachyloban-3‚-ol isolated from the leaves of Croton zambesicus, a plant used in African folk medicine. Materials and Methods: Cell viability on several cell lines, cell morphology, DNA laddering, annexin V and caspase-3 activation experiments were undertaken in order to analyse the cytotoxicty of trachylobane diterpene and to determine if this compound is able to induce apoptosis. Results: ent-trachyloban-3‚-ol exerts a dose-dependent cytotoxic effect and which varies between cell lines. Induction of apoptosis in HL-60 cells could be detected at a concentration of 50 ÌM after 24-h treatment. Conclusion: We show here, for the first time, that a trachylobane diterpene is able to induce apoptosis in human promyelocytic leukemia cells via caspase-3 activation in a concentration-dependent manner.
Disciplines :
Oncology
Author, co-author :
Block, Sébastien; Université Catholique de Louvain - UCL > Unité d'Analyse Chimique et Physico Chimique des Médicaments
Gerkens, Pascal; Université de Liège - ULiège > Histologie et Cytologie
Peulen, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Jolois, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Mingeot-Leclercq, Marie-Paule; Université Catholique de Louvain - UCL > Unité de Pharmacologie Cellulaire et Moléculaire
De Pauw-Gillet, Marie-Claire ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques
Quetin-Leclercq, Joëlle; Université Catholique de Louvain - UCL > Unité d’Analyse Chimique et Physico-Chimique des Médicaments
Language :
English
Title :
Induction of Apoptosis in Human Promyelocytic Leukemia Cells by a Natural Trachylobane Diterpene
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