Phospholipid-Binding And Lecithin-Cholesterol Acyltransferase Activation Properties Of Apolipoprotein-A-I Mutants

[en] Recombinant human apolipoprotein A-I (apo A-I) and three deletion mutants: apo A-I(delta Leu44-Leu126), apo A-I(delta Glu139-Leu170), and apo A-I(delta Ala190-Gln243), purified from the periplasmic space of Escherichia coli, were studied. The rate of turbidity decrease following mixing of apo A-I(delta Ala190-Gln243) with dimyristoylphosphatidylcholine (DMPC) vesicles at 23 degrees C was 10-fold lower than that of the other apo A-I proteins, confirming that the carboxy-terminal region of apo A-I plays a role in rapid lipid binding. The Stokes radii of reconstituted high-density lipoproteins (rHDL), containing dipalmitoylphosphatidylcholine and cholesterol, were larger for the three apo A-I mutants [6.3 nm for apo A-I(delta Leu44-Leu126), 6.1 nm for apo A-I(delta Glu139-Leu170), and 6.5 nm for apo A-I(delta Ala190-Gln243)] than for intact apo A-I (5.0 nm). The mutant rHDL all contained 4 apo A-I molecules per particle as compared to 2 for intact apo A-I. Circular dichroism measurements revealed 8 alpha-helices per apo A-I molecule, 5 per apo A-I(delta Leu44-Leu126), 6 per apo A-I(delta Glu139-Leu170), and 4 per apo A-I(delta Ala190-Gln243) molecule as compared to predicted values of 8, 5, 6, and 6 alpha-helices, respectively.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Holvoet, P.

Zhao, Za.

Vanloo, B.

Vos, R.

Deridder, E.

Dhoest, A.

Taveirne, J.

Brouwers, E.

Demarsin, E.

Engelborghs, Y.

More authors (3 more)

Language :

English

Title :

Phospholipid-Binding And Lecithin-Cholesterol Acyltransferase Activation Properties Of Apolipoprotein-A-I Mutants

Publication date :

1995

Journal title :

Biochemistry

ISSN :

0006-2960

eISSN :

1520-4995

Publisher :

American Chemical Society, Washington, United States - District of Columbia

Volume :

Issue :

Pages :

13334-13342
13334-42

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 June 2010

Statistics

Number of views

52 (2 by ULiège)

Number of downloads

1 (1 by ULiège)

More statistics

Scopus citations^®

Scopus citations^®
without self-citations

OpenCitations

OpenAlex citations

Bibliography

Anantharamaiah, G. M., Jones, J. L., Brouillette, C. G., Schmidt, C. F., Chung, B. H., Hughes, T. A., Bhown, A. S., & Segrest, J. P. (1985) J. Biol. Chem. 260, 10248-10255.
Anantharamaiah, G. M., Venkatachalapathi, Y. V., Brouillette, C. G., & Segrest, J. P. (1990) Arteriosclerosis 10, 95-105.
Barter, P. J., Hopkins, G. J., & Gorjatschko, L. (1985) Atherosclerosis 58, 97-107.
Bolin, D. J., & Jonas, A. (1994) J. Biol. Chem. 269, 7429-7434.
Bradford, M. M. (1976) Anal. Biochem. 72, 248-254.
Brasseur, R. (1991) J. Biol. Chem. 266, 16120-16127.
Brasseur, R., De Meutter, J., Vanloo, B., Goormaghtigh, E., Ruysschaert, J. M., & Rosseneu, M. (1990) Biochim. Biophys. Acta 1043, 245-252.
Breiter, D. R., Kanost, M. R., Benning, M. M., Wesenberg, G., Law, J. H., Wells, M. A., Rayment, I., & Holden, M. M. (1991) Biochemistry 30, 603-608.
Brewer, M. B., Jr., Fairwell, T., LaRue, A., Ronan, R., Houser, A., & Bronzert, T. J. (1978) Biochem. Biophys. Res. Commun. 80, 623-630.
Brouillette, C. G., Jones, J. L., Ng, T. C., Kercret, H., Chung, B. H., & Segrest, J. P. (1984) Biochemistry 23, 359-367.
Calabresi, L., Meng, Q. H., Castro, G. R., & Marcel, Y. L. (1993) Biochemistry 32, 6477-6484.
Chen, Y. H., Yang, J. T., & Martinez, M. M. (1972) Biochemistry 11, 4120-4131.
Cheung, P., & Chan, L. (1983) Nucleic Acids Res. 11, 3703-3715.
Cheung, M. C., Segrest, J. P., Albers, J. J., Cone, J. T., Brouillette, C. G., Chung, B. H., Kashyap, M., Glasscock, M. A., & Anantharamaiah, G. M. (1987) J. Lipid Res. 28, 913-929.
Christian, J. C., Carmelli, D., Castelli, W. P., Fabsitz, R., Grim, C. E., Meaney, F. J., Norton, J. A. Jr., Reed, T., Williams, C. J., & Wood, P. D. (1990) Arteriosclerosis 10, 1020-1025.
Daniels, R. J., Guertler, L. S., Parker, T. S., & Steinberg, D. (1981) J. Biol. Chem. 256, 4978-4983.
De Backer, G., Hulstaert, F., De Munck, K., Rosseneu, M., Van Parijs, L., & Dramaix, M. (1986) Am. Heart J. 112, 478-484.
Dobiasova, M., Stribma, J., Sparks, D. L., Pritchard, P. H., & Frohlich, J. J. (1991) Arterioscler. Thromb. 11, 64-70.
Dobiasova, M., Stribma, J., Pritchard, P. H., & Frohlich, J. J. (1992) J. Lipid Res. 83, 1411-1418.
Dolphin, P. J. (1992) in Structure and Function of Apolipoprotein (Rosseneu, M., Ed.) pp 295-362, CRC Press, Boca Raton, FL.
Fielding, C. J., & Fielding, P. E. (1981) Proc. Natl. Acad. Sci. U.S.A. 78, 3911-3914.
Fielding, C. J., Shore, V. G., & Fielding, P. E. (1972) Biochem. Biophys. Res. Commun. 46, 1493-1498.
Fukushima, D., Yokoyama, S., Kroon, D. J., Kezdy, F. J., & Kaiser, E. T. (1980) J. Biol. Chem. 255, 10651-10657.
Funke, H., von Eckardstein, A., Pritchard, P. H., Albers, J. J., Kastelein, J. J., Droste, C., & Assmann, G. (1991a) Proc. Natl. Acad. Sci. U.S.A. 88, 4855-4859.
Funke, H., von Eckardstein, A., Pritchard, P. H., Karas, M., Albers, J. J., & Assmann, G. (1991b) J. Clin. Invest. 87, 371-376.
Glomset, J. A. (1968) J. Lipid Res. 9, 155-167.
Gordon, D. J., & Rifkind, B. M. (1989) N. Engl. J. Med. 321, 1311-1316.
Hunt, S. C., Hasstedt, S. J., Kuida, H., Stults, B. M., Hopkins, P. N., & Williams, R. R. (1989) Am. J. Epidemiol. 129, 625-638.
Ji, Y., & Jonas, A. (1995) J. Biol. Chem. 270, 11290-11297.
Jonas, A., Kézdy, K. E., & Wald, J. H. (1989) J. Biol. Chem. 264, 4818-4824.
Jonas, A., Steinmetz, A., & Churgay, L. (1993) J. Biol. Chem. 268, 1596-1602.
Koshland, D., & Botstein, D. (1980) Cell 20, 749-760.
Kramer, W., Drutsa, V., Jansen, H. W., Kramer, B., Pflugfelder, M., & Fritz, H. J. (1984) Nucleic Acids Res. 12, 9441-9456.
Kroon, D. J., Kupferberg, J. P., Kaiser, E., & Kezdy, F. J. (1978) J. Am. Chem. Soc. 100, 5975-5977.
Lehrer, S. S. (1971) Biochemistry 10, 3254-3263.
Maniatis, T., Fritsch, E. F., & Sambrook, J. (1982) in Molecular Cloning (A Laboratory Manual), p 200, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
Matz, C. E., & Jonas, A. (1982) J. Biol. Chem. 257, 4535-4540.
Meng, Q. H., Calabresi, L., Fruchart, J. C., & Marcel, Y. L. (1993) J. Biol. Chem. 268, 16966-16973.
Miller, N. E., La Ville, A., & Crook, D. (1985) Nature 314, 109-111.
Minnich, A., Collet, X., Roghani, A., Cladaras, C., Hamilton, R. L., Fielding, C. J., & Zannis, V. I. (1992) J. Biol. Chem. 267, 16553-16560.
Nakagawa, S. H., Lau, H. S. H., Kezdy, F. J., & Kaiser, E. T. (1985) J. Am. Chem. Soc. 107, 7087-7092.
Oram, J. F., Albers, J. J., Cheung, M. C., & Bierman, E. L. (1981) J. Biol. Chem. 256, 8348-8356.
Pometta, D., Micheli, H., Suenram, A., & Jomot, C. (1979) Atherosclerosis 34, 419-429.
Pownall, H., Pao, Q., Hickson, D., Sparrow, J. T., Kusserow, S. K., & Massey, J. B. (1981) Biochemistry 20, 6630-6635.
Rail, S. C., Jr., Weisgraber, K. H., Mahley, R. W., Ogawa, Y., Fielding, C. J., Utermann, G., Haas, J., Steinmetz, A., Menzel, H. J., & Assmann, G. (1984) J. Biol. Chem. 259, 10063-10070.
Roberts, G. C. K. (1981) in Standards in fluorescence spectrometry (Miller, J. N., Ed.) pp 49-67, Chapman and Hall, New York.
Schmidt, M. H. J., Remaley, A. T., Stonik, J. A. Ronan, R., Wellmann, A., Thomas, F., Zech, L. A., Brewer, M. B., Jr., & Hoeg, J. M. (1995) J. Biol. Chem. 270, 5469-5475.
Segrest, J. P., Jackson, R. L., Morrisett, J. D., & Gotto, A. M., Jr. (1974) FEBS Lett. 38, 247-258.
Segrest, J. P., De Loof, H., Dohlman, J. G., Brouillette, C. G., & Anantharamaiah, G. M. (1990) [published erratum appeared in (1991) Proteins: Struct., Fund., Genet. 9, 79]. Proteins: Struct., Fund., Genet. 8, 103-117.
Segrest, J. P., Jones, M. K., De Loof, H., Brouillette, C. G., Venkatachalapathi, Y. V., & Anantharamaiah, G. M. (1992) J. Lipid Res. 33, 141-166.
Shapiro, J. P., Law, J. H., & Wells, M. A. (1988) Annu. Rev. Entomol. 33, 297-318.
Sorci-Thomas, M., Kearns, M. W., & Lee, J. P. (1993) J. Biol. Chem. 268, 21403-21409.
Soutar, A. K., Gamer, C. W., Baker, M. N., Sparrow, J. T., Jackson, R. L., Gotto, A. M., & Smith, L. C. (1975) Biochemistry 14, 3057-3064.
Sparks, D. L., Anantharamaiah, G. M., Segrest, J. P., & Phillips, M. C. (1995) J. Biol. Chem. 270, 5151-5157.
Sparrow, J. T., & Gotto, A. M., Jr. (1980) Ann. N.Y. Acad. Sci. 348, 187-211.
Srinivas, R. V., Venkatachalapathi, Y. U., Rui, Z., Owens, R. J., Gupta, K. B., Srinivas, S. K., Anantharamaiah, G. M., Segrest, J. P., & Compans, R. W. (1991) J. Cell. Biochem. 45, 224-237.
Stanssens, P., Opsomer, C., McKeown, Y. M., Kramer, W., Zabeau, M., & Fritz, H. J. (1989) Nucleic Acids Res. 17, 4441-4454.
Swaney, J. B. (1986) Methods Enzymol. 128, 613-626.
Utermann, G., Haas, J., Steinmetz, A., Paetzold, R., Rail, S. C., Jr., Weisgraber, K. H., & Mahley, R. W. (1984) Eur. J. Biochem. 144, 325-331.
Vanloo, B., Morrison, J., Fidge, N., Lorent, G., Lins, L., Brasseur, R., Ruysschaert, J. M., Baert, J., & Rosseneu, M. (1991) J. Lipid Res. 32, 1253-1264.
Vercaemst, R., Union, A., Rosseneu, M., De Craene, I., De Backer, G., & Komitzer, M. (1989) Atherosclerosis 78, 245-250.
Wald, J. H., Goormaghtigh, E., De Meutter, J., Ruysschaert, J. M., & Jonas, A. (1990) J. Biol. Chem. 265, 20044-20050.
Yanisch-Perron, C., Vieira, J., & Messing, J. (1985) Gene 33, 103-119.
Zhou, C., Yang, Y., & Jong, A. Y. (1990) BioTechniques 8, 172-173.