The C-terminal helix of human apolipoprotein AII promotes the fusion of unilamellar liposomes and displaces apolipoprotein AI from high-density lipoproteins.
[en] To assess the functional properties of apolipoprotein (apo) AII and to investigate the mechanism leading to the displacement of apo AI from native and reconstituted high-density lipoproteins (HDL and r-HDL) by apo AII, wild-type and variant apo AII peptides were synthesized. The wild-type peptides, residues 53-70 and 58-70, correspond to the C-terminal helix of apo AII and are predicted to insert at a tilted angle into a lipid bilayer. We demonstrate that both the apo AII-(53-70) peptide, and to a lesser extent the apo AII-(58-70) peptide are able to induce fusion of unilamellar lipid vesicles together with membrane leakage, and to displace apo AI from HDL and r-HDL. Two variants of the apo AII-(53-70)-wild-type (WT) peptide, designed either to be parallel to the water/lipid interface [apo AII-(53-70)-0 degrees] or to retain an oblique orientation [apo AII-(53-70)-30 degrees], were synthesized in order to test the influence of the obliquity on their fusogenic properties and ability to displace apo AI from HDL. The parallel variant did not bind lipids, due to its self-association properties. However, the apo AII-(53-70)-30 degrees variant was fusogenic and promoted the displacement of apo AI from HDL. Moreover, the extent of fusion of the apo AII-(53-70)-WT, apo AII-(58-70)-WT and apo AII-(53-70)-30 degrees peptides was related to the alpha-helical content of the lipid-bound peptides measured by infrared spectroscopy. Infrared measurements using polarized light also confirmed the oblique orientation of the helical component of the three peptides. In native and r-HDL, the tilted insertion of the C-terminal helix of apo AII resulting in a partial destabilization of the HDL external lipid layer might contribute to the displacement of apo AI by apo AII.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Lambert, Géraldine ; Centre Hospitalier Universitaire de Liège - CHU > Anesthésie et réanimation
Decout, A.
Vanloo, B.
Rouy, D.
Duverger, N.
Kalopissis, A.
Vandekerckhove, J.
Chambaz, J.
Brasseur, Robert ; Université de Liège - ULiège > Gembloux Agro-Bio Tech
Rosseneu, M.
Language :
English
Title :
The C-terminal helix of human apolipoprotein AII promotes the fusion of unilamellar liposomes and displaces apolipoprotein AI from high-density lipoproteins.
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Bibliography
Rosseneu, M., Van Tornout, P., Lievens M. J. & Assman. G. (1981) Displacement of apo A-I by human apo A-II from complexes of apo-A-I-phosphutidylcholine-cholcsterol, Eur. J. Biochem. 117. 347-352.
Edelstein, C., Halari. M. & Scanu. A. M. (1982) On the mechanism of displacement of apolipoprotein A-I by apolipoprotein A-II from the HDI. surface. J. Biol. Chem. 257. 7189-7195.
Lopez, J., Latta. M., Collet. X., Vanloo. B., Jung. G., Denefle. P., Rosseneu. M. & Chambaz, J. (1995) Purification and characterization of recombinant human apolipoprotein A-II expressed in E. coli, Eur J. Biochem. 225, 1141-1150.
Fielding. C. I., Shore. V. G. & Fielding. P. E. (1972) Lecithin: cholesterol acyl transferase: effect of substrate composition upon enzyme activity. Proc. Natl Acad. Sci. USA. 81, 140-144.
Sparrow, J. T. & Gotto. A. M. (1981) Peptide/lipid interaction : studies with synthetic polypeptides. Crit. Rev. Biochem. 13. 87-107.
Marzal-Casacuberta, A., Blanco-Vaca. F., Ishida. B. Y., Julve-Gil. J., Shen. J., Calvet-Marquez. S., Gonzales-Sastre, F. & Chan. L. (1996) Functional LCAT deficiency and HDL deficiency in transgenic mice overexpressing human apolipoprotein A-II. J. Biol. Chem. 271, 6720-6728.
Mowri. H O., Patsch. J. R., Gotto. A. M. & Patsch. W. (1996) Apolipoprotein A-II influences the substrate properties of human HDL2 and HDL3 for hepatic lipase. Arterioscler. Thromb. Vasc. Biol. 16, 755-762.
Jahn, C. E., Osborne, J. C. Jr. Schaefer. E. J. & Brewer, H. B. Jr (1983) Activation of the enzymatic activity of hepatic lipase by apo A-II. Eur. J. Biochem. 131, 25-29.
Thuren, T., Wilcox. R. W., Sisson, P. & Whaite. P. (1991) Hepatic lipase hydrolysis of lipid regulation by apolipoproteins, J. Biol. Chem. 266. 4853-4861.
Huang, Y., Von Eckardstein, A., Wu. S. & Assmann. G. (1995) Cholesterol efflux, cholesterol esterification and cholesteryl ester transfer by Lp A-I and Lp A-I:A-II in native plasma, Arterioscler. Thromb. Vasc. Biol. 15, 1412-1418.
Oikawa. S., Mendez. A. J., Oram. J. F., Bierman, E. L. & Cheung, M. C. (1993) Effects of HDL particles containing apo A-I. with or without apo A-II on intracellular cholesterol efflux. Biochim. Biophys. Acta 1165, 327-334.
Stein. O., Dabach. G., Ben-Naim. M., Oette. K. & Stein. O. (1995) Effects of interactions of apolipoprotein A-II with apolipoproteins A-I and A-IV on cholesterol efflux and uptake in cell culture. Biochim. Biophys. Acta 1257, 174-180.
Schultz. J. R., Verstuyft, J. G., Gong. E. L., Nichols, A. V. & Rubin. E. M. (1993) Protein composition determines the anti-atherogenic properties in transgenic mice. Nature .365, 762-764.
Warden. C. H., Hedrick. C. C., Qiao, J. H., Castellani. L. W. & Lusis. A. J. (1993) Atherosclerosis in transgenic mice overexpressing human apolipoprotein A-II. Science 261, 469-472.
Schultz, J. R. & Rubin, E. M. (1994) The properties of HDL in genetically engineered mice. Curr. Opin. Lipidol. 5. 126- 137.
Barter, P. J. & Rye. K. A. (1996) High Density Lipoprotein and coronary heart disease. Atheroscerosis 121. 1 -12.
Fievet, C., Tailleux. A., Caillaud. J. M., Fruchard. J. C., Denèfle. P. & Duverger, N. (1996) Protective effect of human apo A-II overexpression on atherogenesis in transgenic mice. Circulation 94. 631-632.
Barbaras, R., Puchois. P., Fruchart. J. C. & Ailhaud. G. (1987) Cholesterol efflux from cultured adipose cells is mediated by Lp A-I-particles but not by Lp A-I:A-II-particles. Biochem. Biophys. Res. Commun. 142, 63-69.
Tall, A. R. (1990) Plasma HDL metabolism and relationship to atherosclerosis, J. Clin. Invest. 86. 379-84.
Gong, E. L., Stoltzfus, L. J., Brion. C. M., Murugesh, D. & Rubin. E. M. (1996) Contrasting in vivo effects of murine and human apolipoprotein A-II. J. Biol. Chem. 271, 5984-5987.
Bernini, F., Calabresi, L., Bonfadini, G. & Franceschini. G. (1996) The molecular structure of apolipoprotein A-II modulates the capacity of HDL to promote cholesterol efflux. Biochim. Biophys. Acta 1299, 103-109.
Buchko. O. W., Wang. C., Pifrens. G. K. & Cushley. R. J. (1996) Conformational studies of the amphipathic peptide corresponding to human apolipoprotein A-II residues 18-30 with a C-terminal lipid binding motif EWLNS. Int. J. Peptide Protein Res. 48. 21 - 30.
Benetollo. C., Lambert. G., Talussot. C., Vanloo. B., Van Cauteren. T., Rouy. D., Duhois. H., Baert. J., Kalopissis. A., Denefle. P., Chambaz. J., Brasseur. R. & Rosseneu. M. (1996) Lipid-binding properties of synthetic peptide fragments of human apolipoprotein A-II, Eur. J. Biochem. 242. 657-664.
Brasseur, R., Vandenbranden, M., Cornet, B. & Ruysschaert, J.-M. (1990) Orientation into the lipid bilayer of an asymetric amphipathic helical peptide at the N-terminus of viral fusion protein. Biochim. Biophys. Acta 1029, 267-273.
Horth, M., Lambrecht, B., Khim, M. C. L., Bex, F., Thiriard, C., Ruysschaert, J.-M., Burny, A. & Brasseur, R. (1991) Theoretical and functional analysis of the SIV fusion peptide. EMBO J. 10. 2747-2755.
Martin, I., Dubois, M. C., Defrise-Quertain, F., Saermark, T., Burny, A., Brasseur, R. & Ruysschaert, J.-M. (1994) Correlation between fusogenicity of synthetic modified peptides corresponding to the NH2-terminal extremity of simian immunodeficiency virus gp32 and their mode of insertion into the lipid bilayer: an infrared spectroscopy study. J. Virol. 68. 1139-1148.
Tatulian, S. A., Hinterdorfer, P., Baber, G. & Tamm, L. K. (1995) Influenza hemagglutinin assumes a tilted conformation during membrane fusion as determined by attenuated total reflection FTIR spectroscopy, EMBO J. 14, 5514-5523.
Pillot, T., Goethals, M., Vanloo, B., Talussot, C., Brasseur, R., Vandekerckhove, J., Rosseneu, M. & Lins, L. (1996) Fusogenic properties of the C-terminal domain of the Alzheimer β-amyloid peptide, J. Biol. Chem. 271, 28757-28765.
Brasseur, R., Pillot, T., Lins, L., Vandekerckhove, J. & Rosseneu, M. (1997) Peptide in membranes: tipping the balance of membrane stability. Trends Biochem. Sci. 22, 167-171.
Vonèche, V., Portetelle, D., Kettman, R., Willems, L., Limbach, K., Paoletti, E., Ruysschaert, J.-M., Burny, A. & Brasseur, R. (1992) Fusogenic segments of bovine leukemia virus and simian immunodeficiency virus are interchangeable and mediate fusion by means of oblique insertion in the lipid bilayer of their target cells. Proc. Natl Acad. Sci. USA 89, 3810-3814.
Zimmerberg, J., Vogel, S. S. & Chernomordik, L. V. (1993) Mechanism of membrane fusion, Annu. Rev. Biophys. Biomol. Struct. 22, 433-466.
Rapaport, D., Ovadia, M. & Shai, Y. (1995) A synthetic peptide corresponding to a conserved heptad repeat domain is a potent inhibitor of sendai virus-cell fusion: an emerging similarity with functional domains of other viruses, EMBO J. 14. 5524-5531.
Martin, I., Schaal, H., Scheid, A. & Ruysschaert, J.-M. (1996) Lipid membrane fusion induced by the human immunodeficiency virus type Igp41 N-terminal extremity is determined by its orientation in the lipid bilayer. J. Virol. 70, 298-304.
Colotto, A., Martin, I., Ruysschaert. J.-M., Sen, A., Hui, S. W. & Epand, R. M. (1996) Structural study of the interaction between the SIV fusion peptide and model membranes. Biochemistry 35, 980-989.
Delahunty, M. D., Rhee, I., Freed, E. O. & Bonifacino, J. S. (1996) Mutational analysis of the fusion peptide of the human immunodeficiency virus type 1 : identification of critical glycine residues, Virology 218, 94-102.
Van Tornout, P., Vercaemst, R., Lievens, M. J., Caster, H., Rosseneu, M. & Assman, G. (1980) Reassembly of human apo AI and apo AII proteins with unilamellar phosphatidylcholine cholesterol liposomes, Biochim. Biophys. Acta 601, 509-523.
Vercaemst, R., Union, A. & Rosseneu, M. (1989) Separation and quantitation of free cholesterol and cholesteryl esters in a macrophage cell line by HPLC, J. Chromatogr. 494, 43-52.
Brasseur, R. & Ruysschaert, J.-M. (1986) Conformation and mode of organization of amphiphilic membrane components: a conformational analysis, Biochem. J. 238, 1-11.
Brasseur, R. (1991) Differentiation of lipid associating helices by use of tridimensional molecular hydrophobicity potential calculation, J. Biol. Chem. 266, 16120-16127.
Brasseur, R. (1990) TAMMO: theoretical analysis of membrane molecular organization p95-105, in Molecular description of biological membranes by computer aided conformational analysis (Brasseur, R., ed.) vol. 1, pp. 203-221. CRC Press, Oxford.
Brasseur, R., Vanloo, B., Deleys, R., Lins, L., Labeur, C., Taveirne, J., Ruysschaert, J.-M. & Rosseneu, M. (1993) Design and properties of synthetic model peptides for the amphipathic helices of plasma apolipoproteins, Biochim. Biophys. Acta 1170. 1-7.
Goormaghtigh, E., Cabiaux, V. & Ruysschaert, J.-M. (1990) Secondary structure and dosage of soluble and membrane proteins by attenuated total reflection Fourier transform infrared spectroscopy on hydrated film, Eur. J. Biochem. 193, 409-420.
Cabiaux, V., Brasseur, R., Wattiez., R., Falmagne, P., Ruysschaert, J.-M. & Goormaghtigh, E. (1989) Secondary structure of DT and its fragments interacting with acidic liposomes studied by polarized infrared spectroscopy. J. Biol. Chem. 264, 4928-4938.
Fringeli, U. R. & Günthard M. H. (1981) Infrared membrane spectroscopy, in Membrane spectroscopy (Grell, E., ed.) pp. 270-332, Springer-Verlag, Berlin.
Gremlich, H. U., Fringeli, U. P. & Schwyzer, R. (1983) Conformational changes of adrenocorticotropin peptides upon interaction with lipid membranes revealed by infrared attenuated total reflection spectroscopy, Biochemistry 22, 4257-4264.
Kauppinen, J. K., Moffat, D. J., Cameron, D. G. & Mantsch, H. (1981) Noise in Fourier self deconvolution, Appl. Opt. 20, 1866-1879.
Susi, H. & Byler, D. M. (1986) Examination of the secondary structure of proteins by deconvoluted FTIR spectra, Biopolymers 25, 469-487.
Krimm, S. & Bandekar, J. (1986) Vibrational spectroscopy and conformation of peptides, polypeptides and proteins, Adv. Protein Chem. 38, 181-364.
Defrise-Quertain, F., Cabiaux, V., Vandenbranden, M., Wattiez, R., Falmagne, P. & Ruysschaert, J.-M. (1989) pH-dependent bilayer destabilisation and fusion of phospholipidic large unilamellar vesicles induced by diphteria toxin and its fragments A and B, Biochemistry 28, 3406-3413.
Havel, R. J., Eder, M. A. & Bragdon, J. M. (1955) The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum, J. Clin. Invest. 34, 1345.
Castro, G. & Fielding, C. (1988) Early incorporation of cell derived cholesterol into pre-beta migrating HDL. Biochemistry 27, 25-29.
Matz, C. E. & Jonas, A. (1982) Reaction of human lecithin:cholesterol acyl transferase with synthetic micellar complexes of apolipoproteins A-I, phosphatidylcholine and cholesterol. J. Biol. Chem. 257, 4535-4540.
Morris, S. J., Bradley, D., Gibson, C. C., Smith, P. D. & Blumenthal, R. (1988) Use of membrane associated fluorescence, probes to monitor fusion of bilayer vesicles: application to rapid kinetics using pyrene excimer/monomer fluorescence in Spectroscopic membrane probes I, pp. 161-191, CRC Press, Oxford.
Chen, T., Sparrow, J. T., Gotto, A. M. & Morrisett, J. (1979) Apolipoprotein A-II: chemical synthesis and biophysical properties of three peptides corresponding to fragments in the amino-terminal half, Biochemistry 18, 1617-1626.
Mao, S. J., Jackson, R. L., Gotto, A. M. & Sparrow, J. T. (1981) Mechanism of lipid-protein interactions in plasma apolipoproteins: identification of a lipid binding site in apolipoprotein A-II. Biochemistry 20, 1676-1680.
Vanloo, B., Morrison, J., Fidge, N., Lorent, G., Lins, L., Brasseur, R., Ruysschaert, J.-M., Baert, J. & Rosseneu, M. (1991) Characterization of the discoidal complexes formed between apo A-I-CNBr fragments and phosphatidylcholine, J. Lipid Res. 32, 1253-1264.
Pownall, H. J., Pao, Q., Hickson, D., Sparrow, J. T., Kusserow, S. K. & Massey, J. B. (1981) Kinetics and mechanism of association of human plasma apolipoproteins with dimyristoylphosphatidylcholine: effect of protein structure and lipid clusters on reaction rates, Biochemistry 20, 6630-6635.
Blumenthal, R., Henkart, M. & Steer, C. J. (1983) Clathrin-induced pH-dependent fusion of phosphatidylcholine vesicles, J. Biol. Chem. 258, 3409-3415.
Brauner, J. W., Mendelsohn, R. & Prendergast, F. G. (1987) Attenuated total reflectance Fourier transform infrared studies of the interaction of melittin, two fragments of melittin and δ-hemolysin with phosphatidylcholines, Biochemistry 26, 8151-8158.
Goormaghtigh, E., Martin, I., Vandenbranden, M., Brasseur, R. & Ruysschaert, J.-M. (1989) Secondary structure and orientation of a chemically synthesized mitochondrial signal sequence in phospholipid bilayer, Biochem, Biophys. Res. Commun. 158, 610-616.
Jackson, M., Mantsch, H. H. & Spencer, J. H. (1992) Conformation of magainin-2 and related peptides in aqueous solution and membrane environments probed by Fourier transform infrared spectroscopy. Biochemistry 31, 7289-7293.
Arrondo, J. L. R., Muga, A., Castresana, J. & Goni, F. M. (1993)Quantitative studies of the structure of proteins in solution by Fourier-transform infrared spectroscopy. Prog. Biophys. Mol. Biol. 59, 23-56.
Jackson, M. & Mantsch, H. H. (1995) The use and misuse of FTIR spectroscopy in the determination of protein structure, Crit. Rev. Biochem. Mol. Biol. 30, 95-120.
Lear, J. D. & DeGrado, W. (1987) Membrane binding and conformational properties of peptides representing the NH2 terminus of influenza HA2, J. Biol. Chem. 262, 6500-6505.
Wharton, S. A., Martin, R., Ruigrok, R., Skehel, J. & Wiley, D. (1988) Membrane fusion by peptide analogues of influenza virus hemagglutinin, J. Gen. Virol. 69, 1847-1857.
Rye, C.-A. (1990) Interaction of apolipoprotein A-II with recombinant HDL containing egg phosphatidylcholine, unesterified cholesterol and apolipoprotein A-I, Biochim. Biophys. Acta 1042, 227-236
Forte, T. M., Bielicki, J. K., Goth-Goldstein, R., Selmek, J. & McCall, M. R. (1995) Recruitment of cell phospholipids and cholesterol by apo A-II and A-I: formation of nascent apolipoprotein-specific HDL that differ in size. phospholipid composition and reactivity with LCAT, J. Lipid Res. 36, 148-157.
Pussinen, P. J., Jauhiainen, M. & Enholm, C. (1997) Apo A-II/apo A-I molar ratio in the HDL particle influences phospholipid transfer protein-mediated HDL interconversion. J. Lipid Res. 38. 12-21
Forte, T. M., Bielicki, J. K., Knoff, L. & McCall, M. R. (1996) Structural relationships between nascent apo A-I containing particles that are extracellularly assembled in cell culture, J. Lipid Res. 37, 1076-1085.
Lagrost, L., Persegol, L., Lallemant, C. & Gambert, P. (1994) influence of apolipoprotein composition of HDL particles on CETP activity, J. Biol. Chem. 269, 3189-3197
Lagrost, L., Dengremont, C., Athias. A., de Geitere. C., Fruchart. J.-C., Lallemant, C., Gambert, P. & Castro, G. (1995) Modulation of cholesterol efflux from Fu5AH hepatoma cells by the apolipoprotein content of HDL particles, J. Biol. Chem. 270, 13004-13009.
Eisenberg, D. (1984) Three-dimensional structure of membrane and surface proteins, Annu. Rev. Biochem. 53, 595-623.
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