[en] Under specific conditions, lipid membranes form ripple phases with intriguing
nanoscale undulations. Here, we show using in situ atomic force microscopy (AFM)
that the biologically important surfactin lipopeptide induces nanoripples of 30
nm periodicity in dipalmitoyl phosphatidylcholine (DPPC) bilayers at 25 degrees
(i.e. well below the pretransition temperature of DPPC). Whereas most undulations
formed the classical straight orientation with characteristic angle changes of
120 degrees , some of them also displayed unusual circular orientations.
Strikingly, ripple structures were formed at 15% surfactin but were rarely or
never observed at 5 and 30% surfactin, emphasizing the important role played by
the surfactin concentration. Theoretical simulations corroborated the AFM data by
revealing the formation of stable surfactin/lipid assemblies with positive
curvature.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Brasseur, Robert ; Université de Liège - ULiège > Gembloux Agro-Bio Tech
Braun, N.
El Kirat, K.
Deleu, Magali ; Université de Liège - ULiège > Chimie et bio-industries > Chimie biologique industrielle
Mingeot-Leclercq, Marie-Paule
Dufrene, Yf.
Language :
English
Title :
The Biologically Important Surfactin Lipopeptide Induces Nanoripples In Supported Lipid Bilayers
Publication date :
2007
Journal title :
Langmuir
ISSN :
0743-7463
eISSN :
1520-5827
Publisher :
American Chemical Society, Washington, United States - District of Columbia
Kakinuma, A.; Ouchida, A.; Shima, T.; Sugino, H.; Isono, M.; Tamura, G.; Arima, K. Agric. Biol. Chem. 1969, 33, 1669.
Maget-Dana, R.; Ptak, M. J. Colloid Interface Sei. 1992, 153, 285.
Razafindralambo, H.; Thonart, P.; Paquot, M. J. Surfactants Deterg. 2004, 7, 41.
Vollenbroich, D.; Özel, M.; Vater, J.; Kamp, R. M.; Pauli, G. Biologicals 1997, 25, 289.
Vollenbroich, D.; Pauli, G.; Özel, M.; Vater, J. Appl. Environ. Microbiol. 1997, 63, 44.
Nagle, J. F.; Tristram-Nagle, S. Biochim. Biophys. Acta 2000, 1469, 159.
Heimburg, T. Biophys. J. 2000, 78, 1154.
Leidy, C.; Kaasgaard, T.; Crowe, J. H.; Mouritsen, O. G.; Jorgensen, K. Biophys. J. 2002, 83, 2625.
Kaasgaard, T.; Leidy, C.; Crowe, J. H.; Mouritsen, O. G.; Jorgensen, K. Biophys. J. 2003, 85, 350.
Engel, A.; Müller, D. J. Nat. Struct. Biol. 2000, 7, 715.
Jena, B. P., Hörber, J. K. H., Eds. Atomic Force Microscopy in Cell Biology; Methods in Cell Biology; Academic Press: San Diego, CA, 2002; Vol. 68.
Mou, J. X.; Yang, J.; Shao, Z. F. Biochemistry 1994, 33, 4439.
Mou, J. X.; Czajkowsky, D. M.; Shao, Z. F. Biochemistry 1996, 35, 3222.
Czajkowsky, D. M.; Huang, C.; Shao, Z. F. Biochemistry 1995, 34, 12501.
Fang, Y.; Yang, J. J. Phys. Chem. 1996, 100, 15614.
Dufrêne, Y. F.;Barger, W. R.; Green, J. B. D.; Lee, G. U. Langmuir 1997, 73, 4779-4784.
Dufrêne, Y. F.; Lee, G. U. Biochim. Biophys. Acta 2000, 1509, 14-41.
Rinia, H. A.; Kik, R. A.; Demel, R. A.; Snel, M. M. E.; Killian, J. A.; van der Eerden, J. P. J. M.; de Kruijff, B. Biochemistry 2000, 39, 5852.
Janshoff, A.; Steinern, C. ChemBioChem 2001, 2, 798-808.
Giocondi, M.-C.; Vié, V.; Lesniewska, E.; Milhiet, P.-E.; Zinke-Allmang, M.; Le Grimellec, C. Langmuir 2001, 17, 1653.
Reviakine, I.; Bergsma-Schutter, W.; Morozov, A. N.; Brisson, A. Langmuir 2001, 17, 1680.
Rinia, H. A.; Boots, J. W.; Rijkers, D. T.; Kik, R. A.; Snel, M. M.; Demel, R. A.; Killian, J. A.; van der Eerden, J. P.; de Kruijff, B. Biochemistry 2002, 47, 2814.
Brasseur, R.; Killian, J. A.; de Kruijff, B.; Ruysschaert, J. M. Biochim. Biophys. Acta 1987, 903, 11.
Epand, R. F.; Thomas, A.; Brasseur, R.; Vishwanathan, S. A.; Hunter, E.; Epand, R. M. Biochemistry 2006, 45, 6105.
