Atomic force microscopy of supported lipid bilayers.

[en] Supported lipid bilayers (SLBs) are widely used in biophysical research to investigate the properties of biological membranes and offer exciting prospects in nanobiotechnology. Atomic force microscopy (AFM) has become a well-established technique for imaging SLBs at nanometer resolution. A unique feature of AFM is its ability to monitor dynamic processes, such as the interaction of bilayers with proteins and drugs. Here, we present protocols for preparing dioleoylphosphatidylcholine/dipalmitoylphosphatidylcholine (DOPC/DPPC) bilayers supported on mica using small unilamellar vesicles and for imaging their nanoscale interaction with the antibiotic azithromycin using AFM. The entire protocol can be completed in 10 h.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Mingeot-Leclercq, Marie*-Paule

Deleu, Magali ; Université de Liège - ULiège > Gembloux Agro-Bio Tech

Brasseur, Robert ; Université de Liège - ULiège > Gembloux Agro-Bio Tech

Dufrene, Yves F

Language :

English

Title :

Atomic force microscopy of supported lipid bilayers.

Publication date :

2008

Journal title :

Nature Protocols

ISSN :

1754-2189

eISSN :

1750-2799

Publisher :

Nature Publishing Group, United Kingdom

Volume :

Issue :

Pages :

1654-9

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 23 June 2010

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Scopus citations^®

186

Scopus citations^®
without self-citations

181

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182

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