Reference : Extended-spectrum-beta-lactamase-producing Enterobacteriaceae in Yaounde, Cameroon
Scientific journals : Article
Life sciences : Microbiology
Extended-spectrum-beta-lactamase-producing Enterobacteriaceae in Yaounde, Cameroon
Gangoue Pieboji, Joseph mailto [Institut de Recherches médicales et d'étude des plantes médicinales -IMPM-cameroun > > > >]
Bedenic, B. [> > > >]
Koulla-Shiro, S. [> > > >]
Randegger, C. [> > > >]
Adiogo, D. [> > > >]
Ngassam, P. [> > > >]
Ndumbe, P. [> > > >]
Hachler, H. [> > > >]
Journal of Clinical Microbiology
American Society for Microbiology (ASM)
Yes (verified by ORBi)
[en] Anti-Bacterial Agents/pharmacology ; Cameroon/epidemiology ; Enterobacteriaceae/drug effects/*enzymology/isolation & purification ; Enterobacteriaceae Infections/*epidemiology/microbiology ; Humans ; Microbial Sensitivity Tests/methods ; Molecular Sequence Data ; Sequence Analysis, DNA ; beta-Lactam Resistance ; beta-Lactamases/genetics/*metabolism
[en] Organisms producing extended-spectrum beta-lactamases (ESBLs) have been reported in many countries, but there is no information on the prevalence of ESBL-producing members of the family Enterobacteriaceae in Cameroon. A total of 259 Enterobacteriaceae strains were isolated between 1995 and 1998 from patients at the Yaounde Central Hospital in Cameroon. Enterobacterial isolates resistant to extended-spectrum cephalosporin and monobactam were screened for ESBL production by the double-disk (DD) synergy test. Thirty-one (12%) of these Enterobacteriaceae strains were shown to be positive by the DD synergy test, suggesting the presence of ESBLs. Resistance to oxyimino-cephalosporins and monobactams of 12 (38.7%) of the 31 strains-i.e., 6 Klebsiella pneumoniae, 4 Escherichia coli, 1 Citrobacter freundii, and 1 Enterobacter cloacae strain-was transferred to E. coli HK-225 by conjugation. Resistance to gentamicin, gentamicin plus trimethoprim-sulfamethoxazole, or trimethoprim-sulfamethoxazole was cotransferred into 6, 2, and 1 of these transconjugants, respectively. All 12 transconjugants were resistant to amoxicillin, piperacillin, all of the cephalosporins, and aztreonam but remained susceptible to cefoxitin and imipenem. Crude extracts of beta-lactamase-producing transconjugants were able to reduce the diameters of inhibition zones around disks containing penicillins, narrow- to expanded-spectrum cephalosporins or monobactams when tested against a fully susceptible E. coli strain but had no effect on such zones around cefoxitin, imipenem, and amoxicillin-clavulanate disks. The beta-lactamases produced by the 12 tranconjugants turned out to be SHV-12 by DNA sequencing. Therefore, the ESBL SHV-12 is described for the first time in Cameroon.

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