[en] Organisms producing extended-spectrum beta-lactamases (ESBLs) have been reported in many countries, but there is no information on the prevalence of ESBL-producing members of the family Enterobacteriaceae in Cameroon. A total of 259 Enterobacteriaceae strains were isolated between 1995 and 1998 from patients at the Yaounde Central Hospital in Cameroon. Enterobacterial isolates resistant to extended-spectrum cephalosporin and monobactam were screened for ESBL production by the double-disk (DD) synergy test. Thirty-one (12%) of these Enterobacteriaceae strains were shown to be positive by the DD synergy test, suggesting the presence of ESBLs. Resistance to oxyimino-cephalosporins and monobactams of 12 (38.7%) of the 31 strains-i.e., 6 Klebsiella pneumoniae, 4 Escherichia coli, 1 Citrobacter freundii, and 1 Enterobacter cloacae strain-was transferred to E. coli HK-225 by conjugation. Resistance to gentamicin, gentamicin plus trimethoprim-sulfamethoxazole, or trimethoprim-sulfamethoxazole was cotransferred into 6, 2, and 1 of these transconjugants, respectively. All 12 transconjugants were resistant to amoxicillin, piperacillin, all of the cephalosporins, and aztreonam but remained susceptible to cefoxitin and imipenem. Crude extracts of beta-lactamase-producing transconjugants were able to reduce the diameters of inhibition zones around disks containing penicillins, narrow- to expanded-spectrum cephalosporins or monobactams when tested against a fully susceptible E. coli strain but had no effect on such zones around cefoxitin, imipenem, and amoxicillin-clavulanate disks. The beta-lactamases produced by the 12 tranconjugants turned out to be SHV-12 by DNA sequencing. Therefore, the ESBL SHV-12 is described for the first time in Cameroon.
Disciplines :
Microbiology
Author, co-author :
Gangoue Pieboji, Joseph ; Institut de Recherches médicales et d'étude des plantes médicinales -IMPM-cameroun
Bedenic, B.
Koulla-Shiro, S.
Randegger, C.
Adiogo, D.
Ngassam, P.
Ndumbe, P.
Hachler, H.
Language :
English
Title :
Extended-spectrum-beta-lactamase-producing Enterobacteriaceae in Yaounde, Cameroon
Publication date :
2005
Journal title :
Journal of Clinical Microbiology
ISSN :
0095-1137
eISSN :
1098-660X
Publisher :
American Society for Microbiology (ASM), Washington, United States - District of Columbia
Ambler, R.P., Coulson, A.F., Frere, J.M., Ghuysen, J.M., Joris, B., Forsman, M., Levesque, R.C., Waley, S.G., A standard numbering scheme for the class A β-lactamases (1991) Biochem. J., 276, pp. 269-270
Ariel, G., Philippon, A., Construction by polymerase chain reaction and intragenic DNA probes for three main types of transferable β-lactamases (TEM, SHV, CARB) (1989) FEMS Microbiol. Lett., 82, pp. 19-26
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K., (1994) Current Protocols in Molecular Biology, , John Wiley & Sons, Inc., Chichester, United Kingdom
Bradford, P.A., Automated thermal cycling is superior to traditional methods for nucleotide sequencing of blaSHV genes (1999) Antimicrob. Agents Chemother., 43, pp. 2960-2963
Bradford, P.A., Extended -spectrum β-lactamases in the 21st century: Characterization, epidemiology, and detection of this important resistant threat (2001) Clin. Microbiol. Rev., 14, pp. 933-951
Bush, K., Jacoby, G.A., Medeiros, A.A., A functional classification scheme for β-lactamases and its correlation with molecular structure (1995) Antimicrob. Agents Chemother., 39, pp. 1211-1233
Coudron, P.E., Moland, E.S., Sanders, C.C., Occurrence and detection of extended-spectrum beta-lactamases in members of the family Enterobacteriaceae at a Veterans Medical Center: Seek and you may find (1997) J. Clin. Microbiol., 35, pp. 2593-2597
El-Karsh, T., Tawfik, A.F., Al-Shammary, F., Al-Salah, S., Kambal, A.M., Shibl, A., Antimicrobial resistance and prevalence of extended spectrum β-lactamase among clinical isolates of gram-negative bacteria in Riyadh (1995) J. Chemother., 7, pp. 509-514
Farmer III, J.J., Enterobacteriaceae: Introduction and identification (1999) Manual of Clinical Microbiology, 7th Ed., pp. 442-458. , P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.). American Society for Microbiology, Washington, D.C
Jacoby, G.A., Extended-spectrum β-lactamases and other enzymes providing resistance to oxyimino-β-lactams (1997) Infect. Dis. Clin. N. Am., 11, pp. 875-887
Jarlier, V., Nicolas, M.H., Fournier, G., Philippon, A., Extended broad-spectrum β-lactamases conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae: Hospital prevalence and susceptibility patterns (1988) Rev. Infect. Dis., 10, pp. 867-878
Kayser, F.H., Morenzoni, G., Homberger, F., Activity of cefoperazone against ampicillin-resistant bacteria in agar and broth dilution tests (1982) Antimicrob. Agents Chemother., 22, pp. 15-22
Knothe, A., Shah, P., Krcmery, V., Antal, M., Mitsuhashi, S., Transferable resistance to cefotaxime, cefoxitin, cefamandole and cefuroxime in clinical isolates of Klebsiella pneumoniae and Serratia marcescens (1983) Infection, 11, pp. 315-317
Livermore, D.M., Lactamases in laboratory and clinical resistance (1995) Clin. Microbiol. Rev., 8, pp. 557-584
Martinez-Martinez, L., Hernandez-Alles, S., Alberti, S., Tomas, J.M., Benedi, V.J., Jacoby, G.A., In vivo selection of porin-deficient mutants of Klebsiella pneumoniae with increased resistance to cefoxitin and expanded-spectrum cephalosporins (1996) Antimicrob. Agents Chemother., 40, pp. 342-348
(1999) Performance Standards for Antimicrobial Susceptibility Testing: Ninth Informational Supplement. Approved Standard M100-S9, , NCCLS, Wayne, Pa
Nüesch-Inderbinen, M.T., Hächler, H., Kayser, F.H., Detection of genes coding for extended-spectrum SHV β-lactamases in clinical isolates by a molecular genetic method and comparison with the E test (1996) Eur. J. Clin. Microbiol. Infect. Dis., 15, pp. 398-402
Nüesch-Inderbinen, M.T., Kayser, F.H., Hächler, H., Survey and molecular genetics of SHV-β-lactamases in Enterobacteriaceae in Switzerland: Two novel enzymes, SHV-11 and SHV-12 (1997) Antimicrob. Agents Chemother., 41, pp. 943-949
Philippon, A.M., Paul, G.A., Jacoby, G.A., Properties of PSE-2 β-lactamase and genetic basis for its production in Pseudomonas aeruginosa (1983) Antimicrob. Agents Chemother., 24, pp. 362-369
Randegger, C.C., Keller, A., Irla, M., Wada, A., Hächler, H., Contribution of natural amino acid substitutions in SHV extended-spectrum β-lactamases to resistance against various β-lactams (2000) Antimicrob. Agents Chemother., 44, pp. 2759-2763
Sirot, D., Extended-spectrum plasmid-mediated β-lactamases (1995) J. Antimicrob. Chemother., 36 (SUPPL. A), pp. 19-34
Winokur, P.L., Canton, R., Casellas, J.M., Legakis, N., Variations in the prevalence of strains expressing an extended-spectrum β-lactamase phenotype and characterization of isolates from Europe, the Americas, and the Western Pacific region (2001) Clin. Infect. Dis., 32 (SUPPL. 2), pp. S94-S103
Xiong, Z., Zhu, D., Wang, F., Zhang, Y., Okamoto, R., Inoue, A., Investigation of extended-spectrum β-lactamase in Klebsiella pneumoniae and Escherichia coli from China (2002) Diagn. Microbiol. Infect. Dis., 44, pp. 195-200