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Immortalization of Human Cervical Keratinocytes by Human Papillomavirus Type 33
Gilles, Christine; Piette, Jacques; Rombouts, S. et al.
1993In International Journal of Cancer, 53 (5), p. 872-9
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Abstract :
[en] Ten immortalized cell lines were established by transfection of human cervical keratinocytes (CK) with HPV-33 DNA and some of their characteristics were investigated. The following observations were made: (a) several cell lines have reached over 100 population doublings in vitro; (b) 3 transcripts were observed, 2 being encoded by the E6/E7 open reading frames (ORFs); (c) cytogenetic analyses showed important genetic modifications such as aneuploidy and isochromosome formation of the q arm of chromosome 8; (d) 2 of the 10 cell lines developed colonies in soft agar but none was able to form tumors when injected s.c. into nude mice; (e) Southern analysis suggested that a single copy of HPV-33 is integrated at a single common site within the genome of the 10 cell lines. These immortalized cell lines should be useful for studying mechanisms involved in proliferation, differentiation and neoplastic transformation of CK by HPV-33.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Gilles, Christine ;  Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Rombouts, S.
Laurent, C.
Foidart, Jean-Michel ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie - Obstétrique
Language :
English
Title :
Immortalization of Human Cervical Keratinocytes by Human Papillomavirus Type 33
Publication date :
12 March 1993
Journal title :
International Journal of Cancer
ISSN :
0020-7136
eISSN :
1097-0215
Publisher :
Wiley Liss, Inc., New York, United States - New York
Volume :
53
Issue :
5
Pages :
872-9
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 22 June 2010

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