Article (Scientific journals)
Analytical study of three cystatin C assays and their impact on cystatin C-based GFR-prediction equations.
Delanaye, Pierre; Pieroni, Laurence; Abshoff, Christelle et al.
2008In Clinica Chimica Acta, 398 (1-2), p. 118-24
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Abstract :
[en] BACKGROUND: Cystatin C-based equations are used to estimate GFR. However, three cystatin C immunoassays are on the market. Difference in cystatin C assays could have strong consequences on the accuracy and precision of cystatin C-based equations. We have performed an analytical study of these three assays and studied potential differences between assays on the precision of cystatin C-based equations. METHODS: We have studied imprecision, recovery, linearity and interferences of the three immunoassays (nephelometric assay from Siemens and turbidimetric assays from Dako and Gentian). The impact of differences in cystatin C assays has been studied for the equations published by Levey (Siemens assay) and Grubb (Dako assay). RESULTS: Analytical performance of the Dako assay is slightly less high. For cystatin C values below 2.5 mg/L, no statistical difference is found between results given by the Dako and the Gentian assays. So, both assays can be used in the Grubb equation. Cystatin C results are different with the Siemens assay. The Levey equation, built with the Siemens assay, can only be used with cystatin C values measured with this assay. Using the Dako or Gentian assay results in the Levey equation can lead to differences in estimating GFR up to 6 mL/min/1.73 m2. Differences can reach 9.5 mL/min/1.73 m2 if the Siemens assay is used in the Grubb equation. CONCLUSION: The Siemens and Gentian assays seem analytically more valid than the Dako assay for cystatin C determination. Differences in cystatin C assays can lead to significant differences in cystatin C-based equations. However, these differences seem less important than the differences observed with creatinine and creatinine-based equations.
Disciplines :
Laboratory medicine & medical technology
Urology & nephrology
Author, co-author :
Delanaye, Pierre  ;  Centre Hospitalier Universitaire de Liège - CHU > Néphrologie
Pieroni, Laurence
Abshoff, Christelle
Lutteri, Laurence ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Chapelle, Jean-Paul ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Krzesinski, Jean-Marie ;  Centre Hospitalier Universitaire de Liège - CHU > Néphrologie
Hainque, Bernard
Cavalier, Etienne  ;  Centre Hospitalier Universitaire de Liège - CHU > Chimie médicale
Language :
English
Title :
Analytical study of three cystatin C assays and their impact on cystatin C-based GFR-prediction equations.
Publication date :
2008
Journal title :
Clinica Chimica Acta
ISSN :
0009-8981
eISSN :
1873-3492
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
398
Issue :
1-2
Pages :
118-24
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
All our thank to Elsevier
Available on ORBi :
since 16 February 2009

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