[en] Splenic germinal center reactions were measured during primary response to a plasmidic DNA intramuscular injection. Cardiotoxin-pretreated Balb/c mice were immunized with DNA plasmids encodmg or not the SAG1 protein, a membrane antigen of Toxoplasma gondii. Specific anti-SAG1 antibodies were detected on days 16 and 36 after injection of coding plasmids. The results of ELISAs showed that the SAG1-specific antibodies are of the IgG2a class. Morphometric analyses were done on serial immunostained cryosections of spleen and draining or non-draining lymph nodes. This new approach made it possible to evaluate the chronological changes induced by DNA immunisation in the germinal centres (in number and in size). Significant increases in the number of germinal centres were measured in the spleen and only in draining lymph nodes after plasmid injection, the measured changes of the germinal centers appeared to result from the adjuvant stimulatory effect of the plasmidic DNA since both the coding and the noncoding plasmid DNA induced them. No measurable changes were recorded in the T-dependent zone of lymph organs.
Disciplines :
Immunology & infectious disease
Author, co-author :
Ehirchiou, D.
Zorzi, Willy ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine - Département des sciences biomédicales et précliniques
Biemans, R.
Vanderbeeke, A.
Haumont, M.
Collin, S.
Jolois, Olivier ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Bollen, A.
Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Antoine, Nadine ; Université de Liège - ULiège > Département de morphologie et pathologie > Histologie
Language :
English
Title :
DNA immunisation. New histochemical and morphometric data.
Arthur M.K., Ae-Kyung Y., Joachim S., and Davis L.H.: The role of CpG dinucleotides in DNA vaccines. Trends in microbiology 6, 23-26, 1998.
Butcher E.C., Reichert R.A., Coffman R.L., Nottenburg C., and Weissman I.L.: Surface phenotype and migratory capability of Peyer's patch germinal center cells. Adv. Exp. Med. Biol. 149, 765-772, 1982.
Dennis M.K., Galina Y., and Yoshiaki, I.: Contribution of CpG motifs to the immunogenicity of DNA vaccines. J. Immunol. 158, 3635-3639, 1997.
Donnelly J.J., Ulmer J.B., Shiver J.W., and Liu M.A.: DNA vaccines. Annu. Rev. Immuno. 115, 617-648, 1997.
Dupuis M., Denis-Mize K., Woo C., Goldbeck C., Selby M.J., Chen M., Otten G.R., Ulmer J.B., Donnelly J.J., Ott G., and McDonald D.M.: Distribution of DNA vaccines determines their immunogenicity after intramuscular injection in mice. J. Immunol. 165(5), 2850-2858, 2000.
Heinen, E.: Germinal center In encyclopedia of life sciences. Nature, Scientific American, London, http:www.els.net, 1999.
Hollowood K., and Macartney J.: Cell kinetics of the germinal center reaction - a stathmokinetic study. Eur. J. Immunol. 22, 261-266, 1992.
Gallucci S., and Matzinger P.: Danger: Signals: SOS to the immune system. Curr.opin. Immunol. 13, 114-119, 2001.
Kelsoe, G: Life and death in germinal centers (redux). Immunity 4, 107-111, 1996.
Levy M.Y., Barron L.G., Meyer K.B., and Szoka F.C.: Characterization of plasmid DNA transfer into mouse skeletal muscle: evaluation of uptake mechanism, expression and secretion of gene products into blood. Gene Ther. 3(3), 201-211, 1996.
Manthorpe M., Cornefert-Jensen F., Hartikka J., Felgner J., Rundell A., Margalith M., and Dwarki V.: Gene therapy by intramuscular injection of plasmid DNA: studies on firefly luciferase gene expression in mice. Hum. Gene. Ther. 4(4), 419-431, 1993.
McHeyzer-Williams L.J., Driver D.J., and McHeyzer-Williams M.G.: Germinal center reaction. Curr. Opin. Hematol. 8, 52-59, 2001.
Mena A., Andrew M.E., and Coupar E.H.: Rapid dissemination of intramuscularly inoculated DNA vaccines. Immunol. Cell Biol. 79, 87-89, 2001.
Pabst R., and Fritz F.J.: Comparison of lymphocyte production in lymphoid organs and their compartments using the metaphase-arrest technique. Cell. Tissue Res. 245(2), 423-430, 1986.
Sallusto F., and Lanzavecchia A.: Efficient presentation of soluble antigen by cultured human dendritic cells is maintained by granulocyte/macrophage colony-stimulating factor plus interleukine-4 and downregulated by tumor necrosis factor-α. J. Exp. Med. 179, 1109-1118, 1994.
Zhang J., MacLennan I.C., Liu Y.J., and Lane P.J.: Is rapid proliferation in B centroblasts linked to somatic mutation in memory B cell clones? Immunol. Lett. 18(4), 297-299, 1988,
