[en] Recent studies have demonstrated the angiogenic potential of 45S5 Bioglass (R). However, it is not known whether the angiogenic properties of Bioglass (R) remain when the bioactive glass particles are incorporated into polymer composites. The objectives of the current study were to investigate the angiogenic properties of 45S5 Bioglass (R) particles incorporated into biodegradable polymer composites. In vitro studies demonstrated that fibroblasts Cultured on discs consisting of specific quantities of Bioglass (R) particles mixed into poly(D,L-lactide-co-glycolide) secreted significantly increased quantities of vascular endothelial growth factor. The optimal quantity of Bioglass (R) particles determined from the in vitro experiments was incorporated into three-dimensional macroporous poly(D,L-lactide-co-glycolide) foam scaffolds. The foam scaffolds were fabricated using either compression molding or thermally induced phase separation processes. The foams were implanted subcutaneously into mice for periods Of Lip to 6 weeks. Histological assessment was used to determine the area of granulation tissue around the foams, and the number of blood vessels within the granulation tissue was counted. The presence of Bioglass (R) particles in the foams produced a sustained increase in the area of granulation tissue surrounding the foams. The number of blood vessels surrounding the neat foams was reduced after 2 weeks of implantation; however, compression-molded foams containing Bioglass (R) after 4 and 6 weeks of implantation had significant]), more blood vessels surrounding the foams compared with foams containing no Bioglass (R) at the same time points. These results indicate that composite polymer foam scaffolds containing Bioglass (R) particles retain granulation tissue and blood vessels surrounding the implanted foams. The use of this polymer composite for tissue engineering scaffolds might provide a novel approach for ensuring adequate vascular Supply to the implanted device.
Research Center/Unit :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Chemistry Materials science & engineering
Author, co-author :
Day, Richard M; Burdett Institute of Gastrointestinal Nursing, King’s College, London, St. Mark’s Hospital, Harrow, UK > Biomaterials and Tissue Engineering Group
Maquet, Véronique; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Boccaccini, Aldo R.; Imperial College, London, UK > Department of Materials and Centre for Tissue Engineering
Jérôme, Robert ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Forbes, Alastair; Imperial College, London, UK > Faculty of Medicine
Language :
English
Title :
In vitro and in vivo analysis of macroporous biodegradable poly(D,L-lactide-co-glycolide) scaffolds containing bioactive glass
Publication date :
15 December 2005
Journal title :
Journal of Biomedical Materials Research. Part A
ISSN :
1549-3296
eISSN :
1552-4965
Publisher :
Wiley Liss, Inc., Hoboken, United States - New Jersey
The Medical Research Council Discipline Hopper Award scheme, the Kati Jacobs Appeal, and Rosetrees (T. R. Golden Trust) BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
Stock U, Vacanti J. Tissue engineering: current state and prospects. Annu Rev Med 2001;52:443-451.
Mikos AG, Thorsen AJ, Czerwonka LA, Bao Y, Langer R, Winslow DN, Vacanti JP. Preparation and characterization of poly(L-lactic acid) foams. Polymer 1994;35:1068-1077.
Wang HT, Palmer H, Linhardt RJ, Flanagan DR, Schmitt E. Degradation of poly(ester) microspheres. Biomaterials 1990;11:679-685.
Mikos AG, Sarakinos G, Leite SM, Vacanti JP, Langer R. Laminated three-dimensional biodegradable foams for use in tissue engineering. Biomaterials 1993;14:323-330.
Agrawal CM, McKinney JS, Lanctot D, Athanasiou KA. Effects of fluid flow on the in vitro degradation kinetics of biodegradable scaffolds for tissue engineering. Biomaterials 2000;21:2443-2452.
Boccaccini AR, Maquet V. Bioresorbable and bioactive polymer/ Bioglass® composites with tailored pore structure for tissue engineering applications. Comp Sci Technol 2003;63:2417-2429.
Nam YS, Park TG. Porous biodegradable polymeric scaffolds prepared by thermally induced phase separation. J Biomed Mater Res 1999;47:8-17.
Lo H, Kadiyala S, Guggino SE, Leong KW. Poly(L-lactic acid) foams with cell seeding and controlled-release capacity. J Biomed Mater Res 1996;30:475-484.
Schugens C, Maquet V, Grandfils C, Jerome R, Teyssie P. Polylactide macroporous biodegradable implants for cell transplantation. 2. Preparation of polylactide foams by liquid-liquid phase separation. J Biomed Mater Res 1996;30:449-461.
Whang K, Thomas CH, Healy KE, Nuber GA. A novel method to fabricate bioabsorbable scaffolds. Polymer 1995;36:837-842.
Mikos AG, Bao Y, Cima LG, Ingber DE, Vacanti JP, Langer R. Preparation of poly(glycolic acid) bonded fiber structures for cell attachment and transplantation. J Biomed Mater Res 1993;27:183-189.
Freed L, Marquis JC, Nohria A, Emmanual J, Mikos AG. Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. J Biomed Mater Res 1993;27:11-23.
Harris LD, Kim BS, Mooney DJ. Open pore biodegradable matrices formed with gas foaming. J Biomed Mater Res 1998;42:396-402.
Mooney DJ, Baldwin DF, Suh NP, Vacanti JP, Langer R. Novel approach to fabricate porous sponges of poly(D,L-lactic-co-glycolic acid) without the use of organic solvents. Biomaterials 1996;17:1417-1422.
Day RM, Boccaccini AR, Shurey S, Roether JA, Forbes A, Hench LL, Gabe SM. Assessment of polyglycolic acid mesh and bioactive glass for soft-tissue engineering scaffolds. Biomaterials 2004;25:5857-5866.
Piskin E. Biodegradable polymeric matrices for bioartificial implants. Int J Artif Organs 2002;25:434-440.
Marra KG, Choi D, Boduch K. Synthesis of new composites for bone tissue engineering: biodegradable polymers, dendrimers and ceramics. Proceedings of the 222nd ACS National Meeting, Chicago, IL, 2001.
Verrier S, Blaker JJ, Maquet V, Hench LL, Boccaccini AR. PDLLA/Bioglass® composites for soft-tissue and hard-tissue engineering: an in vitro cell biology assessment. Biomaterials 2004;25:3013-3021.
Maquet V, Boccaccini AR, Pravata L, Notingher I, Jerome R. Porous poly(alpha-hydroxyacid)/Bioglass® composite scaffolds for bone tissue engineering. I. Preparation and in vitro characterisation. Biomaterials 2004;25:4185-4194.
Hench LL, Splinter RJ, Allen WC, Greenlee TK. Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res 1971;2:117-141.
Keshaw H, Forbes A, Day R. Release of angiogenic growth factors from cells encapsulated in alginate beads with bioactive glass. Biomaterials 2005;26(19):4171-4179.
Maquet V, Martin D, Scholtes F, Franzen R, Schoenen J, Moonen G, Jerome R. Poly(D,L-lactide) foams modified by poly(ethylene oxide)-block-poly(D,L- lactide) copolymers and a-FGF: in vitro and in vivo evaluation for spinal cord regeneration. Biomaterials 2001;22:1137-1146.
Schugens C, Maquet V, Grandfils C, Jerome R, Teyssie P. Biodegradable and macroporous polylactide implants for cell transplantation. 1. Preparation of macroporous polylactide supports by solid-liquid phase separation. Polymer 1996;37:1027-1038.
Schugens C, Maquet V, Grandfils C, Jerome R, Teyssie P. Polylactide macroporous biodegradable implants for cell transplantation. II. Preparation of polylactide foams by liquid-liquid phase separation. J Biomed Mater Res 1996;30:449-461.
Fox SB. Microscopic assessment of angiogenesis in tumours. In: Murray JC, editor. Angiogenesis protocols: methods in molecular medicine. Totowa, NJ: Humana Press; 2001. p 29-46.
Chou L, Firth JD, Ditto VJ, Brunette DM. Substratum surface topography alters cell shape and regulates fibronectin mRNA level, mRNA stability, secretion and assembly in human fibroblasts. J Cell Sci 1995;108:1563-1573.
Lee KY, Peters MC, Mooney DJ. Comparison of vascular endothelial growth factor and basic fibroblast growth factor on angiogenesis in SCID mice. J Controlled Release 2003;21;87:49-56.
Tarnawski A, Szabo IL, Husain SS, Soreghan B. Regeneration of gastric mucosa during ulcer healing is triggered by growth factors and signal transduction pathways. J Physiol Paris 2001;95:337-344.
