[en] The hGH-V gene codes for a variant of human pituitary growth hormone (hGH-N) named placental growth hormone (hPGH). hPGH shares 93% amino acid identity with hGH-N. Until now the hGH-V gene was considered to be exclusively expressed in human placenta, where it replaces maternal circulating hGH-N at the end of pregnancy. In this study we investigated by reverse transcriptase-polymerase chain reaction (RT-PCR) analysis hGH-N, and hGH-V, gene expression in PBMC in men, women and pregnant women. We have demonstrated that hGH-N and hGH-V transcripts are simultaneously produced by PBMC in both men and women as well as pregnant women. The PBMC of a PIT-1-negative woman expressed only the hGH-V transcript, but not the hGH-N one as expected. In conclusion, hGH-V mRNA is expressed by cells other than the syncytiotrophoblast, is not regulated by PIT-1, and may be involved in immune regulation, as is pituitary GH.
Disciplines :
Immunology & infectious disease
Author, co-author :
Melen-Lamalle, Laurence ; Université de Liège - ULiège > Département des sciences cliniques > Labo de biologie des tumeurs et du développement
Hennen, Georges ; Centre Hospitalier Universitaire de Liège - CHU > Endocrinologie clinique
Dullaart, R. P.
Heinen, Ernst ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie humaine
Igout, Ahmed ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Département des sciences biomédicales et précliniques
Language :
English
Title :
Both Pituitary and Placental Growth Hormone Transcripts Are Expressed in Human Peripheral Blood Mononuclear Cells (Pbmc)
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