Poster (Scientific congresses and symposiums)
Development of a strategy to study toxicodynamic of pollutants in spawning sea turtles from the French West Indies
Dyc, Christelle; Bouquegneau, Jean-Marie; De Pauw-Gillet, Marie-Claire et al.
200815th Benelux Congress of Zoology
 

Files


Full Text
October 2008_BCZ.pdf
Author postprint (685.43 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Abstract :
[en] Sea turtles including the green turtle Chelonia mydas and the hawksbill turtle Eretmochelys imbricata are critically endangered species, facing different factors as marine pollution. There is a blatant lack of data dealing with toxicants such as metals and persistent organic pollutants (POPs) in sea turtles. We developed a strategy to apprehend levels, effects and transfer to offspring of several pollutants in sea turtles. Sampling of blood, subcutaneous tissue and eggs of 15 gravid C. mydas and E. imbricata was carried out between July and September 2008 in Martinique (Diamant’s beach) and Guadeloupe (Petite Terre and Marie-Galante). Blood was collected from the dorso-cervical sinus and subcutaneous tissue was sampled in shoulder of the spawning females using a 5 mm biopsy punch (Kai Europe GmbH, Germany). Total blood and serum were successfully taken for metal, POP and biomarker investigations. T-mercury was analyzed by DMA milestones while PCBs, DDT and chlordecone were analyzed by EDC Ni63 high performance gas chromatography HPLC. Samples of serum were analyzed for vitamins (A and E) by HPLC and for thyroid hormones (triiodothyronine and thyroxine) by radioimmunoassay. In parallel to this field study, a cell model using 3T3-L1 cell line was built up to test in vitro effects of PCBs and mercury as well as the relationship between in vitro exposure and fat mobilization. Preliminary results showed a dose-response relationship between increased Aroclor 1234 and 1252 concentrations (0.5 ppb, 1 ppb and 1.5 ppb) and adipocyte mortality (Nucleocounter). The strategy we propose here will bring further insights on levels and potential impact of pollutants on female sea turtles and their offspring.
Disciplines :
Zoology
Author, co-author :
Dyc, Christelle ;  Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Océanologie
Bouquegneau, Jean-Marie ;  Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Océanologie
De Pauw-Gillet, Marie-Claire ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques
Thomé, Jean-Pierre ;  Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Ecologie animale et écotoxicologie
Das, Krishna  ;  Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Océanologie
Other collaborator :
Cayol, Claire;  Office National de la Chasse et de la Faune Sauvage (ONCFS), Martinique, French West Indies > Veterinary and Sea Turtles Official Representative
Delcroix, Eric;  Association Kap 'Natirel > Guadeloupe, French West Indies > Guadeloupe Sea Turtle Conservation Project Coordinator
Language :
English
Title :
Development of a strategy to study toxicodynamic of pollutants in spawning sea turtles from the French West Indies
Publication date :
October 2008
Number of pages :
88.1 cm x 118.9 cm
Event name :
15th Benelux Congress of Zoology
Event organizer :
ULg - Université de Liège
Event place :
Liege, Belgium
Event date :
30 - 31 October 2008
Audience :
International
Name of the research project :
Toxicodynamic of pollutants in poikilotherm species (turtles)
Available on ORBi :
since 07 June 2010

Statistics


Number of views
149 (11 by ULiège)
Number of downloads
5 (3 by ULiège)

Bibliography


Similar publications



Contact ORBi