Protein crystallization; Microgravity; Atomic resolution; Parvalbumin; Calcium binding protein
Abstract :
[en] We report on our recent observation that crystals of a typical EF-hand protein (parvalbumin or Pa; Ca-loaded component from pike muscle with isoelectric point 4.10) grown under microgravity conditions diffract X-rays to a resolution better than 0.9 Å. The crystals were grown in the US space shuttle and characterized at 100 K, using an X-ray synchrotron beam. An effective atomic resolution has been achieved and substates in the conformation of the protein are observed. Large crystals up to 3 mm were also obtained.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Declercq, Jean-Paul; Université Catholique de Louvain - UCL > Département de Chimie > Unité de Chimie Physique Moléculaire et de Cristallographie - CPMC
Evrard, Christine ; Université Catholique de Louvain - UCL > Département de Chimie > Unité de Chimie Physique Moléculaire et de Cristallographie - CPMC
Carter, Daniel; New Century Pharmaceuticals, Huntsville
Wright, Brenda; New Century Pharmaceuticals, Huntsville
Etienne, Gérard; UPRESA, Montpellier
Parello, Joseph; The Burnham Institute, La Jolla
Language :
English
Title :
A crystal of a typical EF-hand protein grown under microgravity diffracts X-rays beyond 0.9 Å resolution
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Bibliography
E.H. Snell, S. Weisgerber, J.R. Helliwell, E. Weckert, K. Hölzer, K. Schroer, Acta Crystallogr. D 51 (1995) 1099.
M.R. Wardell, R. Skinner, D.C. Carter, P.D. Twigg, J.-P. Abrahams, Acta Crystallogr. D 53 (1997) 622.
R. Skinner, J.-P. Abrahams, J. Whisstock, A.M. Lesk, R.W. Carrell, M.R. Wardell, J. Mol. Biol. 266 (1997) 601.
J.D. Ng, B. Lorber, R. Giegé, S. Koszelak, J. Day, A. Greenwood, A. McPherson, Acta Crystallogr. D 53 (1997) 724.
J.P. Declercq, B. Tinant, J. Parello, Acta Crystallogr. D 52 (1996) 165.
B.W. Matthews, J. Mol. Biol. 33 (1968) 491.
J.P. Declercq, B. Tinant, J. Parello, G. Etienne, R. Huber, J. Mol. Biol. 202 (1988) 349.
J.P. Declercq, B. Tinant, J. Parello, J. Rambaud, J. Mol. Biol. 220 (1991) 1017.
F. Roquet, J.P. Declercq, B. Tinant, J. Rambaud, J. Parello, J. Mol. Biol. 223 (1992) 705.
A. Padilla, A. Cavé, J. Parello, J. Mol. Biol. 204 (1988) 995.
J.F. Pechère, in: R.H. Wasserman, R. Corradino, E. Carafoli, R.H Kretsinger, D.H. MacLennan, F.L. Siegel (Eds.), Calcium Binding Proteins and Calcium, Elsevier, North-Holland, New York, 1977, p. 213.
Y. Blancuzzi, A. Padilla, J. Parello, A. Cavé, Biochemistry 32 (1993) 1302.
A. Cavé, C.M. Dobson, J. Parello, R.J.P. Williams, FEBS Lett. 65 (1976) 190.
A. Cavé, J. Parello, in: Les Houches, Session XXXIII, 1979, Membranes and Intercellular Communication, North-Holland, Amsterdam, 1981, p. 197.
D.C. Carter, T.E. Dowling, Protein Crystallization Apparatus for Microgravity, U.S. Patent No. 5,643,540, 1997.
F.C. Bernstein, T.F. Koetzle, G.J.B. Williams, E.F. Meyer, Jr., M.D. Brice, J.R. Rodgers, O. Kennard, T. Shimanouchi, M. Tasumi, J. Mol. Biol. 112 (1977) 535.
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