[en] 17alpha-ethynylestradiol sex-reversed males of Oreochromis aureus (pseudofemales, DeltaF, genotype ZZ) are used in aquaculture to produce a male monosex population by crossing with ZZ homogametic normal males. When placed with males (M) and females (F) in the same spawning tank, the spawning rate of F is higher than for DeltaF. In order to understand this phenomenon, comparisons were made between the behaviour of 18 F (446 +/- 96 mm) and 18 DeltaF (401 +/- 59 mm). DeltaF showed a more aggressive behaviour and were significantly more dominant than normal F in fighting pair experiments (F x DeltaF) or in fighting group experiments in four different stocking densities (8, 12, 16 and 83 fish 10(-3) l with F/DeltaF ratio = 1). DeltaF were also more aggressive towards males than F were. The results support the idea that behavioural differences exist between F and DeltaF These differences are probably due to the effect of the sexual genotype on behaviour. (C) 2002 Ifremer/CNRS/Inra/IRD/Cemagref/Editions scientifiques et medicales Elsevier SAS. All rights reserved.
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Ovidio, Michaël ; Université de Liège - ULiège > Biologie du comportement - Ethologie et psychologie animale
Desprez, Damien
Mélard, Charles ; Université de Liège - ULiège > Centre de formation et de recherche en aquaculture (CEFRA)
Poncin, Pascal ; Université de Liège - ULiège > Biologie du comportement - Ethologie et psychologie animale
Language :
English
Title :
Influence of sexual genotype on the behaviour of females (genotype WZ) and pseudofemales (genotype ZZ) in the tilapia Oreochromis aureus
Afonso, L.O.B., Wasserman, G.J., De Oliveira, R.T., 2001. Sex reversal in Nile tilapia (Oreochromis niloticus) using a nonsteroidal aromatase inhibitor. J. Exp. Zool. 290, 177-181.
Baroiller, J.F., Guiguen, Y., Fostier, A., 1999. Endocrine and environmental aspects of sex differentiation in fish. Cell. Mol. Life Sci. 55, 910-931.
Chervinsky, J., Rothbard, S., 1982. An aid in manually sexing tilapia. Aquaculture 26, 389.
Desprez, D., Mélard, C., Philippart, J.C., 1994. Production of high percentage male offspring with 17α-ethynyloestradiol sex reversed Oreochromis aureus: II. Comparative reproductive biology of females and F2 pseudofemales and large scale production of male progenies. Aquaculture 130, 175-190.
Desprez, D., Mélard, C., 1998. Influence du génotype sexuel sur les caractéristiques de la reproduction de femelles (génotype WZ) et de pseudofemelles (génotype ZZ) chez le tilapia Oreochromis aureus. Aquat. Living Resour. 11, 145-153.
Falter, U., 1986. Fluctuations journalières dans le comportement territorial chez Oreochromis niloticus (Teleostei: Cichlidae). Ann. Soc. R. Belg. 2, 175-190.
Forselius, S., 1957. Studies on anabantid fishes. Zool. Bidr. Uppsala 32, 593-597.
Hulata, G., Wolfarth, G.W., Karplus, I., Schroder, G.L., Harpaz, S., Halevy, A., Rothbard, S., Cohan, S., Israel, I., Kavessa, M., 1993. Evaluation of Oreochromis niloticus x Oreochromis aureus hybrid progeny of different geographical isolates, reared under varying management regimes. Aquaculture 115, 253-271.
Iwamatsu, T., 1999. Convenient method for sex reversal in a freshwater teleost, the Medaka. J. Exp. Zool. 283, 210-214.
Jensen, G.L., Shelton, W.L., 1979. Effects of estrogens on Tilapia aurea: Implication for production of monosex genetic male Tilapia. Aquaculture 16, 133-242.
Johns, L.S., Lilely, N.R., Seghens, B.H., 1969. The effects of gonadectomy on the reproductive behaviour of the blue gourami, Trichogaster trichopterus (cited in Hoar, W.S.,1969) Reproduction. In: Hoar, W.S., Randall, D.J. (Eds.), Fish Physiology, Vol. 3. Academic Press, New York, pp. 1-72.
Kavumparth, S., Pandian, T.J., 1990. Induction of triploid in the zebra fish Brachydanio rerio. Aquac. Fish. Manag. 21, 219-306.
Kwon, J.H., McAndrew, B.J., Penman, D.J., 2001. Cloning of brain aromataze gene and expression of brain and ovarian aromatase genes during sexual differentiation in genetic male and female nile tilapia Oreochromis niloticus. Mol. Reprod. Dev. 59, 359-370.
Levy, M., Aronson, L.R., 1955. Morphological effects of castration and hormone administration in the male cichlid fish Tilapia macrocephala. Anat. Rec. 122, 450-451.
Mac Bay, L.G., 1961. The biology of Tilapia nilotica (Linnaeus). Proc. Ann. Conf. Southeast Assoc. Game Fish Comm. 15, 208-218.
Mair, G.C., Penman, D.J., Scott, A., Skibinski, D.O.F., Beardmore, J.A., 1987. Hormonal sex reversal and the mechanisms of sex determination in Oreochromis. Proc. World Symp. Selection, Hybridisation and Genetic Engineering in Aquaculture, Vol. 2, pp. 27-30.
Mélard, C., 1995. Production of high percentage of male offspring with 17α-ethynyloestradiol sex reversed Oreochromis aureus: I. Estrogen sex-reversal and production of F2 pseudofemales. Aquaculture 130, 25-34.
Meriwether, F.H., Torrans, E.K., 1986. Evaluation of new androgen (mibolerone) and procedure to induce functional sex reversal in tilapia. In: Mclean, J.L., Dizon, L.B., Hosillos, L.V. (Eds.), Proc. First Asian Fish. Soc. Manila, Philippines. pp. 675-678.
Mires, D., 1982. A study of the problem of mass production of hydrid Tilapia fry. In: Pullin, R.S.V., Lowe-McConnel, R.H. (Eds.), The Biology and Culture of Tilapia, ICLARM Conference Proceeding 7, Manilla, Philippines. pp. 317-329.
Monfort, A., Ruwet, J.C., 1968. Patrons moteurs et mouvements d'expression chez les Tilapia rendalii (Boulenger, 1896) et Tholloni congica (Poissons Cichlidés). Rev. Zool. Bot. Afr. 77 (1-2), 59-68.
Neat, F.C., Huntingford, F.A., Beveridge, M.M.C., 1998. Fighting and assessment in male cichlid fish: The effects of asymmetries in gonadal state and body size. Anim. Behav. 55, 883-891.
Oliveira, R.F., Carneiro, L.A., Canario, A.V.M., Grober, M.S., 2001. Effect of androgens on social behaviour and morphology of alternative reproductive males azorean rock-pool blenny. Hormone Behav. 39, 157-166.
Pandian, T.J., Sheela, S.G., 1995. Hormonal induction of sex reversal in fish. Aquaculture 138, 1-22.
Philippart, J.C., Ruwet, J.C., 1982. Ecology and distribution of tilapias. In: Pullin, R.S.V., Lowe-Mc Connel, R.H. (Eds.), The Biology and Culture of Tilapia, ICLARM Conference Proceeding 7, Manilla, Philippines. pp. 15-59.
Schwanck, E., 1980. The effect of size and Hormonal state on the establishment of dominance in young males of Tilapia-Mariae (Pisces, Cichlidae). Behav. Processes 5, 45-53.
Shelton, W.L., Hopkins, K.D., Jensen, G.L., 1978. Use of hormones to produced monosex Tilapia for aquaculture. In: Smitherman, R.O., Shelton, W.L., Grover, S.H. (Eds.), Culture of Exotic Fishes Symposium Proceedings. Fish Culture Section. Am. Fish. Soc. Auburn. pp. 10-33.
Schumacher, M., Balthazart, J., 1986. Testosterone-induced brain aromatase is sexually dimorphic. Brain Res. 370, 285-293.
Tayamen, M.M., Shelton, W.L., 1979. Inducement of sex reversal in Sarotherodon niloticus (Linnaeus). Aquaculture 14, 349-354.
Varadaraj, K., 1989. Feminization of Oreochromis mossambicus by the administration of diethylstilbestrol. Aquaculture 80, 337-341.
