[en] The purpose of the present study was to screen 27 plant species used in the traditional medicine of Cambodia for in vitro antibacterial and antifungal activities. Thirty-three methanolic extracts were tested against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Mycobacterium smegmatis and Candida albicans. Screened by disk diffusion assay, the extracts showed antimicrobial activity especially on Gram-positive bacteria. None of the crude methanolic extracts showed activity against P. aeruginosa. Twenty-five selected extracts were evaluated using a micro-dilution test. Harrisonia perforata (roots) and Hymenodictyon excelsum (bark) exhibited a bactericidal effect against S. aureus at a concentration of 500 μg/ml. Azadirachta indica (bark), Harrisonia perforata (roots and stem) and Shorea obtusa (roots) exhibited a bactericidal effect against M. smegmatis at 250 μg/ml.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Chea, Aun; Université de la Méditerrannée
Jonville, Marie ; Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Bun, Sok-Siya; Université de la Méditerrannée
Laget, Michèle; Université de la Méditerrannée
Elias, Riad; Université de la Méditerrannée
Duménil, Gérard; Université de la Méditerrannée
Balansard, Guy; Université de la Méditerrannée
Language :
English
Title :
In vitro antimicrobial activity of plants used in Cambodian traditional medicine
Publication date :
2007
Journal title :
American Journal of Chinese Medicine
ISSN :
0090-2942
Publisher :
Institute for Advanced Research in Asian Science and Medicine, Garden City, United States - New York
Bauer, A.W., W.M. Kirby, J.C. Sherris and M. Turck. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45: 493-496, 1966.
Dy Phon, P. Dictionnaire des Plantes Utilisées au Cambodge. Olympic, Phnom Penh, 2000.
Herrera, R.M., M. Perez, D.A. Martin-Herrera, R. Lopez-Garcia, R.M. Rabanal ajkd A. Arias. Antimicrobial activity of extracts from plants endemic to the Canary Islands. Phytother Res. 10: 364-366, 1996.
Jin, J.M., Y.J. Zhang and C.R. Yang. Four new steroid constituents from the waste, residue of fibre separation from Agave americana leaves. Chem. Pharm. Bull. 52: 654-658, 2004.
Kamiuchi, K., K. Mitsunaga, K. Koike, Y. Ouyang, T. Ohmoto and T. Nikaido. Quassinoids and limonoids from Harrisonia perforata. Heterocycles 43: 653-664, 1996.
Khuong-Huu, Q., A. Chiaroni, C. Riche, H. Nguyen-Ngoc, K. Nguyen-Viet and F. Khuong Huu. New rearranged limonoids from Harrisonia perforata. J. Nat. Prod. 63: 1015-1018, 2000.
Limsong, J., E. Benjavongkulchai and J. Kuvatanasuchati. Inhibitory effect. of some herbal extracts on adherence of Streptococcus mutans. J. Ethnopharmacol. 92: 281-289, 2004.
NCCLS (National Committee for Clinical Laboratory Standards). M7-T2-NCCLS, Villanova, PA. Order from NCCLA, 771 East Lancaster Ave., Villanova, PA 19085, 2000.
Petelot, A. Les Plantes Médicinales du Cambodge, du Laos et du Vietnam. Centre de Recherches Scientifiques et Techniques. I.D.E.O Saigon, 1952.
Rates, S.M.K. Plants as source of drugs. Toxicon 39: 603-613, 2001.
Rios, J.L., M.C. Recio and A. Villar Screening methods for natural products with antimicrobial activity: A review of the literature. J. Ethnopharmacol. 23: 127-149, 1988.
SaiRam, M., G. Ilavazhagan, S.K. Sharma, S.A. Dhanraj, B. Suresh, M.M. Parida, A.M. Jana, K. Devendra, and W. Selvamurthy. Anti-microbial activity of a new vaginal contraceptive NIM-76 from neem oil (Azadirachta indica). J. Ethnopharmacol. 71: 377-382, 2000.
Tinto, W.F., J.L. Simmons-Boyce, S. McLean and W.F. Reynolds. Constituents of Agave americana and Agave barbadensis. Fitoterapia 76: 594-597, 2005.
Van der Nat, J.M., W.G. van der Sluis, K.T.D. de Silva and R.P. Labadie. Ethnopharmacognostical survey of Azadirachta indica A. Juss (Meliaceae). J. Ethnopharmacol. 35: 1-24, 1991.
