[en] The antifouling properties of poly(2-hydroxyethyl methacrylate-co-methyl methacrylate) hydrogels were improved by the surface grafting of a brush of poly(oligoethylene glycol methyl ether acrylate) [poly(OEGA)]. The atom-transfer radical polymerization (ATRP) of OEGA (degree of polymerization = 8) was initiated from the preactivated surface of the hydrogel under mild conditions, thus in water at 25 °C. The catalytic system was optimized on the basis of two ligands [1,1,4,7,10,10-hexamethyl-triethylenetetramine (HMTETA) or tris[2-(dimethylamino)ethyl]amine (Me6TREN)] and two copper salts (CuIBr or CuICl). Faster polymerization was observed for the Me6TREN/CuIBr combination. The chemical composition and morphology of the coated surface were analyzed by X-ray photoelectron spectroscopy, attenuated total reflectance Fourier transform infrared spectroscopy, contact angle measurements by the water droplet and captive bubble methods, scanning electron microscopy, and environmental scanning electron microscopy. The hydrophilicity of the surface increased with the molar mass of the grafted poly(OEGA) chains, and the surface modifications were reported in parallel. The antifouling properties of the coatings were tested by in vitro protein adsorption and cell adhesion tests, with green fluorescent protein, β-lactamase, and lens epithelial cells, as model proteins and model cells, respectively. The grafted poly(OEGA) brush decreased the nonspecific protein adsorption and imparted high cell repellency to the hydrogel surface.
Research center :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Chemistry Materials science & engineering
Author, co-author :
Bozukova, Dimitriya ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
The Walloon Region of Belgium in the framework of the First Europe (SURFOLIO) program BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Feng, W.; Zhu, S.; Ishihara, K.; Brash, J. L. Biointerphases 2006, 1, 50-60.
Feng, W.; Brash, J. L.; Zhu, S. Biomaterials 2006, 27, 847-855.
Jayachandran, K. N.; Takacs-Cox, A.; Brooks, D. E. Macromolecules 2002, 35, 4247-4257.
Boyes, S. G.; Brittain, W. J.; Weng, X.; Cheng, S. Z. D. Macromolecules 2002, 35, 4960-4967.
Yang, Q.; Tian, J.; Hu, M. X.; Xu, Z. K. Langmuir 2007, 23, 6684-6690.
Ciampolini, M.; Nardi, N. Inorg. Chem. 1966, 5, 41.
Bozukova, D.; Pagnoulle, C.; De Pauw-Gillet, M. C.; Ruth, N.; Jérôme, R.; Jérôme, C. Biomacromolecules 2007, 8, 2379-2387.
Luo, Y.; Dalton, P. D.; Shoichet, M. S. Chem. Mater. 2001, 13, 4087-4093.
Dreesen, L.; Humbert, C.; Sartenaer, Y.; Caudano, Y.; Volcke, C.; Mani, A. A.; Peremans, A.; Thiry, P. A.; Hanique, S.; Frère; J, M. Langmuir 2004, 20, 7201-7207.
(a) Xu, F.; Zhen, G.; Yu, F.; Kuennemann, E.; Textor, M.; Knoll, W. J. Am. Chem. Soc. 2005, 127, 13084.
(b) Gabriel, S.; Cécius, M.; Fleury-Frenette, K.; Cossement, D.; Hecq, M.; Ruth, N.; Jérôme, R.; Jérôme, C. Chem. Mater. 2007, 19, 2364-2371.
DumaineL. Correlation Entre les Proprietes Physico-Chimiques de Polymeres Permettant de Moduler la Proliferation Cellulaire et Celles Permettant de Favoriser le Glissement de Lentilles Intraoculaires. Thesis, Ecole Centrale de Lyon, 2004,79-85.
Linnola, R.; Salonen, J. I.; Happonen, R. P. J. Cataract Refract. Surg. 1999, 25, 1480-1485.
Yamamoto, K.;Miwa, Y.;Tanaka, H.; Sakaguchi,M.; Shimada, S. J. Pol. Sci., Part A: Pol. Chem. 2002, 40, 3350-3359.