[en] Varicella-zoster virus (VZV) open reading frame 4-encoded protein (IE4) possesses transactivating properties for VZV genes as well as for genes of heterologous viruses, The major regulatory immediate-early protein of VZV (IE62) is a transactivator of VZV gene expression, In transfection assays, IE4 has been shown to enhance activation induced by IE62, To investigate the functional interactions underlying this observation, indirect immunofluorescence studies were undertaken to determine whether IE62 could influence IE4 intracellular localization in transfected cells, In single transfections, IE4 was predominantly found in cytoplasm, In cotransfection with IE62, the IE4 localization pattern was altered, with nuclear staining predominating over cytoplasmic staining, This effect was specific to the IE62 protein since the gene products of ORF63 and ORF61, which are also regulatory proteins, did not influence IE4 distribution, The use of IE62 mutants indicated that IE62 influence is independent of its transactivation function and that the integrity of regions 3 and 4 is required, IE62 remained nuclear whether IE4 was present or not, These observations underline differences in the regulation of gene expression between VZV proteins and their herpes simplex virus type 1 homologues, In infected cells, IE4 was only sometimes found to colocalize with IE62 in nuclei, This observation suggests that when all VZV proteins are present, complex interactions probably occur which could diminish the influence of IE62.
Disciplines :
Immunology & infectious disease
Author, co-author :
Defechereux, Patricia; Université de Liège - ULiège > Département des Sciences de la Vie > Virologie fondamentale & Immunologie
Debrus, Serge; Université de Liège - ULiège > Département des Sciences de la Vie > Virologie fondamentale & Immunologie
Baudoux, Laurence; Université de Liège - ULiège > Département des Sciences de la Vie > Virologie fondamentale & Immunologie
Schoonbroodt, Sonia; Université de Liège - ULiège > Département des Sciences de la Vie > Virologir fondamentale & Immunologie
Merville, Marie-Paule ; Université de Liège - ULiège > Département de pharmacie > Chimie médicale
Rentier, Bernard ; Université de Liège - ULiège > Département des Sciences de la Vie > Virologie fondamentale et Immunologie
Piette, Jacques ; Université de Liège - ULiège > Département des sciences de la vie > Virologie fondamentale & Immunologie
Language :
English
Title :
Intracellular distribution of the ORF4 gene product of varicella-zoster virus is influenced by the IE62 protein
Publication date :
July 1996
Journal title :
Journal of General Virology
ISSN :
0022-1317
eISSN :
1465-2099
Publisher :
Society for General microbiology
Volume :
77
Issue :
Part 7
Pages :
1505-1513
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
Version deposited must replicate that published and can be downloaded from the publisher's website
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