biomaterial; nanomedicine; carbon nanoparticle; self-assembly in solution
Abstract :
[en] To improve the efficiency of orally delivered vaccines, PEGylated PLGA-based nanoparticles displaying RGD molecules at their surface were designed to target human M cells. RGD grafting was performed by an original method called "photografting" which covalently linked RGD peptides mainly on the PEG moiety of the PCL-PEG, included in the formulation. First, three non-targeted formulations with size and zeta potential adapted to M cell uptake and stable in gastro-intestinal fluids, were developed. Their transport by an in vitro model of the human Follicle associated epithelium (co-cultures) was largely increased as compared to mono-cultures (Caco-2 cells). RGD-labelling of nanoparticles significantly increased their transport by co-cultures. due to interactions between the RGD ligand and the I intregrins detected at the apical surface of co-cultures. In vivo studies demonstrated that RGD-labelled nanoparticles particularly concentrated in M cells. Finally, ovalbumin-loaded nanoparticles were orally administrated to mice and induced an IgG response, attesting antigen ability to elicit an immune response after oral delivery.
Research Center/Unit :
Center for Education and Research on Macromolecules (CERM)
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Garinot, Marie; The Catholic University of Louvain (UCL) > Unité de Pharmacie Galénique
Fievez, Virginie; The Catholic University of Louvain (UCL) > Unité de Pharmacie Galénique et Laboratoire de Biochimie cellulaire
Pourcelle, Vincent; The Catholic University of Louvain (UCL) > Unité de Chimie Organique et Médicinale
Stoffelbach, François; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
des Rieux, Anne; The Catholic University of Louvain (UCL) > Unité de Pharmacie Galénique et Laboratoire de Biochimie cellulaire
Plapied, Laurence; The Catholic University of Louvain (UCL) > Unité de Pharmacie Galénique et Laboratoire de Biochimie cellulaire
Theate, Ivan; The Catholic University of Louvain (UCL), Cliniques Universitaires Saint-Luc > Department of Pathology
Freichels, Hélène ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Jérôme, Christine ; Université de Liège - ULiège > Department of Chemistry > Center for Education and Research on Macromolecules (CERM)
Marchand-Brynaert, Jacqueline; The Catholic University of Louvain (UCL) > Unité de Chimie Organique et Médicinale
Schneider, Yves-Jacques; The Catholic University of Louvain (UCL) > Unité de Chimie Organique et Médicinale
Preat, Véronique; The Catholic University of Louvain (UCL) > Unité de Pharmacie Galénique
Language :
English
Title :
PEGylated PLGA-based nanoparticles targeting M cells for oral vaccination
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