Article (Scientific journals)
Development of an enzyme-linked immunosorbent assay for specific equine neutrophil myeloperoxidase measurement in blood
Franck, Thierry; Grulke, Sigrid; Deby-Dupont, Ginette et al.
2005In Journal of Veterinary Diagnostic Investigation, 17 (5), p. 412-419
Peer Reviewed verified by ORBi
 

Files


Full Text
JVDI-EIA.pdf
Publisher postprint (110.99 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
ELISA; equine myeloperoxidase; intestinal disease; neutrophil; plasma
Abstract :
[en] Equine inflammatory disease is accompanied by a neutrophil activation resulting in the release of granulocytic enzyme myeloperoxidase (MPO). To measure MPO in horse plasma as marker of neutrophil activation, the authors purified equine neutrophil MPO and developed a specific enzyme immunoassay using 2 specific polyclonal antibodies obtained from rabbit (primary antibody) and guinea pig (secondary antibody). The sandwich complex "primary antibody-MPO-secondary antibody" was detected using a goat anti-guinea pig immunoglobulin antibody conjugated to alkaline phosphatase. The enzyme-linked immunosorbent assay (ELISA) showed good precision and accuracy, with intra- and interassay coefficients of variation below 10% for MPO concentrations ranging from 0.78 to 50 ng/ml. A stable plasma MPO value, unaffected by time elapsed between blood collection and centrifugation, was obtained with plasma from EDTA anticoagulated blood. The mean MPO value measured in 38 healthy horses was 181.80 +/- 64.74 ng/ml. In 20 horses suffering from obstruction of the large or small intestine, MPO concentrations measured at the time of arrival at the intensive care unit were significantly higher than mean normal value, ranging from 477.88 to 2,748.13 ng/ml. Work is in progress to apply this MPO ELISA technique to other biological fluids and other equine diseases.
Research center :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Franck, Thierry  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Grulke, Sigrid  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Deby-Dupont, Ginette;  Université de Liège - ULiège > Centre de l'Oxygène : Recherche et Développement (C.O.R.D.)
Deby, Carol;  Centre de Biochimie de l'Oxygène
Duvivier, Hania
Péters, Fabrice
Serteyn, Didier  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Development of an enzyme-linked immunosorbent assay for specific equine neutrophil myeloperoxidase measurement in blood
Publication date :
September 2005
Journal title :
Journal of Veterinary Diagnostic Investigation
ISSN :
1040-6387
Publisher :
Amer Assoc Veterinary Laboratory Diagnosticians Inc, Turlock, United States - California
Volume :
17
Issue :
5
Pages :
412-419
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
MPOEQUI
Funders :
DGTRE - Région wallonne. Direction générale des Technologies, de la Recherche et de l'Énergie [BE]
Available on ORBi :
since 02 February 2009

Statistics


Number of views
72 (6 by ULiège)
Number of downloads
3 (3 by ULiège)

Scopus citations®
 
62
Scopus citations®
without self-citations
14
OpenCitations
 
55

Bibliography


Similar publications



Contact ORBi