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Cytological Comparison of Leaves and Stems of Prunus Avium L. Shoots Cultured on a Solid Medium with Agar or Gelrite
Franck, Thierry; Crevecoeur, Michelle; Wuest, J. et al.
1998In Biotechnic and Histochemistry, 73 (1), p. 32-43
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Keywords :
gelrite; light and electron microscopy; Prunus avium L.; vitrification; wild cherry
Abstract :
[en] An axillary proliferating clone of Prunus avium L. was subcultured every four weeks on solid MS medium with agar as the gelling agent. Vitrification (hyperhydricity) of shoots was induced in one four week cycle with the same medium except that agar was replaced by gelrite. During culture on the vitrifying medium, the water content of the shoots progressively increased with a parallel decrease in chlorophyll content. Cytological differences between the leaves and stems of the vitrified and normal shoots were detected by light and electron (both transmission and scanning) microscopy. Leaves of vitrified shoots were characterized by lower number of chloroplasts in the palisade parenchyma and by a defective cuticle. The stems of vitrified shoots had a less developed and lignifled xylem tissue, lacked sclerenchymatic areas and showed hypertrophy of the cortical parenchyma. More intense vacuolar activity with evaginations of the chloroplast envelope into the vacuole was noted in cells of vitrified leaves.
Research center :
Fundamental and Applied Plant Hormonology
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Franck, Thierry  ;  Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Crevecoeur, Michelle
Wuest, J.
Greppin, Hubert
Gaspar, Thomas ;  Botanique, radiobotanique & serres expér.
Language :
English
Title :
Cytological Comparison of Leaves and Stems of Prunus Avium L. Shoots Cultured on a Solid Medium with Agar or Gelrite
Publication date :
January 1998
Journal title :
Biotechnic and Histochemistry
ISSN :
1052-0295
eISSN :
1473-7760
Publisher :
Williams and Wilkins
Volume :
73
Issue :
1
Pages :
32-43
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
Luxembourg Ministry of National Education
Available on ORBi :
since 02 February 2009

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