Article (Scientific journals)
Gene activation by varicella-zoster virus IE4 protein requires its dimerization and involves both the arginine-rich sequence, the central part, and the carboxyl-terminal cysteine-rich region
Baudoux, Laurence; Defechereux, Patricia; Rentier, Bernard et al.
2000In Journal of Biological Chemistry, 275 (42), p. 32822-32831
Peer Reviewed verified by ORBi
 

Files


Full Text
Baudoux_L_2000_JBC_275_32822.pdf
Author postprint (709.76 kB)
Download

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Varicella-Zoster virus; Viral Latency; Viral replication control
Abstract :
[en] Varicella-zoster virus (VZV) open reading frame 4-encoded protein (IE4) possesses transactivating properties for VZV genes as well as for those of heterologous viruses. Since most transcription factors act as dimers, IE4 dimerization was studied using the mammalian two-hybrid system. Introduction of mutations in the IE4 open reading frame demonstrated that both the central region and the carboxyl-terminal cysteine-rich domain were important for efficient dimerization. Within the carboxyl-terminal domain, substitution of amino acids encompassing residues 443-447 totally abolished dimerization. Gene activation by IE4 was studied by transient transfection with an IE4 expression plasmid and a reporter gene under the control of either the human immunodeficiency virus, type 1, long terminal repeat or the VZV thymidine kinase promoter. Regions of IE4 important for dimerization were also shown to be crucial for transactivation. In addition, the arginine-rich domains Rb and Re of the amino-terminal region were also demonstrated to be important for transactivation, whereas the Ra domain as well as an acidic and bZIP-containing regions were shown to be dispensable for gene transactivation. A nucleocytoplasmic shuttling of IE4 has also been characterized, involving a nuclear localization signal identified within the Rb domain and a nuclear export mechanism partially depending on Crm-1.
Disciplines :
Immunology & infectious disease
Author, co-author :
Baudoux, Laurence
Defechereux, Patricia
Rentier, Bernard  ;  Université de Liège - ULiège > Département de Microbiologie > Virologie fondamentale et Immunologie
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Language :
English
Title :
Gene activation by varicella-zoster virus IE4 protein requires its dimerization and involves both the arginine-rich sequence, the central part, and the carboxyl-terminal cysteine-rich region
Publication date :
20 October 2000
Journal title :
Journal of Biological Chemistry
ISSN :
0021-9258
eISSN :
1083-351X
Publisher :
American Society of Biochemistry and Molecular Biology
Volume :
275
Issue :
42
Pages :
32822-32831
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 30 January 2009

Statistics


Number of views
63 (3 by ULiège)
Number of downloads
140 (0 by ULiège)

Scopus citations®
 
19
Scopus citations®
without self-citations
17
OpenCitations
 
17

Bibliography


Similar publications



Contact ORBi