[en] Gastrointestinal disorders, especially strangulating intestinal obstructions, are still a major cause of illness and death in the horse. Circulating lipopolysaccharides may activate both neutrophils and monocytes. The activated neutrophils release myeloperoxidase (MPO), a specific enzyme with strong oxidative activity. The aim of this study was to evaluate MPO concentrations in the plasma and peritoneal fluid (PF) of horses with colic and to check the hypothesis that these concentrations would be higher in a case of strangulating obstruction than in cases of nonstrangulating disease. By using a specific enzyme-linked immunosorbent assay for equine MPO, we determined the MPO concentrations in horses admitted to a clinic for colic. Horses with nonstrangulating or strangulating obstruction of the large intestine (NSLI or SLI), strangulating obstruction of the small intestine (SSI), or inflammatory bowel disease (IBD) were compared with healthy horses. The horses with SLI, SSI, or IBD had significantly higher MPO levels in plasma and PF than did those in the other 2 groups. The mean plasma level was significantly higher in the horses with NSLI than in the healthy horses. High MPO values in PF indicated necrotic bowel. These results show that neutrophil activation occurs during nonstrangulating and strangulating intestinal obstruction in horses and that the plasma and PF MPO concentrations may be a marker of the severity of the disease.
Research Center/Unit :
CORD - Centre de l'Oxygène, Recherche et Développement - ULiège
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Grulke, Sigrid ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Franck, Thierry ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Gangl, Monika
Péters, Fabrice
Salciccia, Alexandra ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Département clinique des animaux de compagnie et des équidés
Deby, Ginette ; Centre Hospitalier Universitaire de Liège - CHU > Anesthésie et réanimation
Serteyn, Didier ; Université de Liège - ULiège > Département clinique des animaux de compagnie et des équidés > Anesthésiologie gén. et pathologie chirurg. des grds animaux
Language :
English
Title :
Myeloperoxidase Assay in Plasma and Peritoneal Fluid of Horses with Gastrointestinal Disease
Cohen ND, Matejka PL, Honnas CM, Hooper RN. Case-control study of the association between various management factors and development of colic in horses. Texas Equine Colic Study Group. J Am Vet Med Assoc 1995;206:667-673.
Tinker MK, White NA, Lessard P, et al. Prospective study of equine colic risk factors. Equine Vet J 1997;29:454-458.
Moore JN, White NA, Berg JN, Trim CM, Garner HE. Endotoxemia following experimental intestinal strangulation obstruction in ponies. Can J Comp Med 1981;45:330-332.
King J, Gerring E. Detection of endotoxin in cases of equine colic. Vet Rec 1988;123:269-271.
Benbarek H, Deby-Dupont G, Caudron I, et al. Interaction between lipopolysaccharides and blood factors on the stimulation of equine polymorphonuclear neutrophils. Vet Immunol Immunopathol 1998;64:313-322.
Dagleish MR, Brazil TJ, Scudamore CL. Potentiation of the extracellular release of equine neutrophil elastase and alpha-l-proteinase inhibitor by a combination of two bacterial cell wall components: fMLP and LPS. Equine Vet J 2003;35: 35-39.
Moore RM, Bertone AL, Bailey MQ, Muir WW, Beard WL. Neutrophil accumulation in the large colon of horses during lowflow ischemia and reperfusion. Am J Vet Res 1994;55:454-463.
Partrick DA, Moore FA, Moore EE, Barnett CC Jr, Silliman CC. Neutrophil priming and activation in the pathogenesis of post-injury multiple organ failure. New Horiz 1996;4:194-210.
Deby-Dupont G, Deby C, Lamy M. Neutrophil myeloperoxidase revisited: its role in health and disease. Intensivmed Notfallmed 1999;36:500-513.
Hoy A, Leininger-Muller B, Kutter D, Siest G, Visvikis S. Growing significance of myeloperoxidase in non-infectious diseases. Clin Chem Lab Med 2002;40:2-8.
McConnico RS, Weinstock D, Poston ME, Roberts MC. Myeloperoxidase activity of the large intestine in an equine model of acute colitis. Am J Vet Res 1999;60:807-813.
Weiss DJ, Evanson OA. Evaluation of activated neutrophils in the blood of horses with colic. Am J Vet Res 2003;64:364-1368.
Franck T, Grulke S, Deby-Dupont G, et al. Design and development of an enzyme-linked immunosorbent assay for specific equine neutrophil myeloperoxidase measurement in blood. J Vet Diagn Invest 2005;17:412-419.
Deby-Dupont G, Grulke S, Caudron I, et al. Equine myeloperoxidase in plasma: design of a radio-immunoassay and first results in septic pathologies. Vet Immunol Immunopathol 1998;66:257-271.
Grulke S, Benbarek H, Caudron I, et al. Plasma myeloperoxidase level and polymorphonuclear leukocyte activation in horses suffering from large intestinal obstruction requiring surgery: preliminary results. Can J Vet Res 1999;63:142-147.
Yarbrough B, Snyder JR, Harmon FA, O'Connell KA. Evaluation of myeloperoxidase concentrations in experimentally induced equine colonic ischaemia and reperfusion. Equine Vet J 1994; 26:67-69.
Vandenplas ML, Moore JN, Barton MH, Roussel AJ, Cohen ND. Concentrations of serum amyloid A and lipopolysaccharide-binding protein in horses with colic. Am J Vet Res 2005;66:1509-1516.
Freden GO, Provost PJ, Rand WM. Reliability of using results of abdominal fluid analysis to determine treatment and predict lesion type and outcome for horses with colic: 218 cases (1991-1994). J Am Vet Med Assoc 1998;213:1012-1015.
Matthews S, Dart AJ, Reid SW, Dowling BA, Hodgson DR. Predictive values, sensitivity and specificity of abdominal fluid variables in determining the need for surgery in horses with an acute abdominal crisis. Aust Vet J 2002;80:132-136.
Latson KM, Nieto JE, Beldomenico PM, Snyder JR. Evaluation of peritoneal fluid lactate as a marker of intestinal ischaemia in equine colic. Equine Vet J 2005;37:342-346.
Little D, Tomlinson JE, Blikslager AT. Post operative neutrophilic inflammation in equine small intestine after manipulation and ischaemia. Equine Vet J 2005;37:329-335.
Hurley DH, Parks RJ, Reber AJ, et al. Dynamic changes in circulating leukocytes during the induction of equine laminitis with black walnut extract. Vet Immunol Immunopathol 2006;110:195-206.
Riggs LM, Franck T, Moore JN, et al. Neutrophil myeloperoxidase measurements in plasma, laminar tissue, and skin of horses given black walnut extract. Am J Vet Res 2007;68:81-86.
Tobler A, Koeffler HP. Myeloperoxidase: localization, structure, and function. In: Harris GR, ed. Blood Cell Biochemistry. 3. Lymphocytes and Granulocytes. New York: Plenum Press, 1991:255-288.
Mathy-Hartert M, Deby-Dupont G, Deby C, Jadoul L, Vandenberghe A, Lamy M. Cytotoxicity induced by neutrophil myeloperoxidase towards human endothelial cells: protection by ceftazidime. Mediators Inflamm 1995;4:1-7.
Suter PM, Suter S, Girardin E, Roux-Lombard P, Grau GE, Dayer JM. High bronchoalveolar levels of tumor necrosis factor and its inhibitors, interleukin-1, interferon, and elastase, in patients with adult respiratory distress syndrome after trauma, shock, or sepsis. Am Rev Respir Dis 1992;145:1016-1022.
Franck T, Kohnen S, Deby-Dupont G, Grulke S, Deby C, Serteyn D. A specific method for measurement of equine active myeloperoxidase in biological samples and in in vitro tests. J Vet Diagn Invest 2006;18:326-334.
