Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex.

Hand, Randal; Bortone, Dante; Mattar, Pierre; Nguyen, Laurent; Heng, Julian Ik-Tsen; Guerrier, Sabrice; Boutt, Elizabeth; Peters, Eldon; Barnes, Anthony P; Parras, Carlos; Schuurmans, Carol; Guillemot, Francois; Polleux, Franck

doi:10.1016/j.neuron.2005.08.032

Article (Scientific journals)

Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex.

Hand, Randal; Bortone, Dante; Mattar, Pierre et al.

2005 • In Neuron, 48 (1), p. 45-62

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https://hdl.handle.net/2268/4937

DOI
10.1016/j.neuron.2005.08.032

PubMed
16202708

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Keywords :

Age Factors; Animals; Basic Helix-Loop-Helix Transcription Factors/metabolism; Blotting, Western/methods; Cell Count/methods; Cell Movement/physiology; Cells, Cultured; Chickens; Cloning, Molecular/methods; Dendrites/physiology; Electrophoresis, Gel, Pulsed-Field/methods; Electroporation/methods; Embryo, Mammalian; Embryo, Nonmammalian; Female; Fluorescent Antibody Technique/methods; Gene Expression Regulation, Developmental/physiology; Green Fluorescent Proteins/metabolism; Humans; Male; Mice; Microscopy, Confocal/methods; Microtubule-Associated Proteins/metabolism; Models, Biological; Neocortex/cytology/embryology/metabolism; Nerve Tissue Proteins/metabolism; Phosphorylation; Pregnancy; Pyramidal Cells/cytology/physiology; Sequence Alignment; Stem Cells/physiology; Time Factors; Tubulin/metabolism; Tyrosine/metabolism; rhoA GTP-Binding Protein/metabolism

Abstract :

[en] The molecular mechanisms specifying the dendritic morphology of different neuronal subtypes are poorly understood. Here we demonstrate that the bHLH transcription factor Neurogenin2 (Ngn2) is both necessary and sufficient for specifying the dendritic morphology of pyramidal neurons in vivo by specifying the polarity of its leading process during the initiation of radial migration. The ability of Ngn2 to promote a polarized leading process outgrowth requires the phosphorylation of a single tyrosine residue at position 241, an event that is neither involved in Ngn2 direct transactivation properties nor its proneural function. Interestingly, the migration defect observed in the Ngn2 knockout mouse and in progenitors expressing the Ngn2(Y241F) mutation can be rescued by inhibiting the activity of the small-GTPase RhoA in cortical progenitors. Our results demonstrate that Ngn2 coordinates the acquisition of the radial migration properties and the unipolar dendritic morphology characterizing pyramidal neurons through molecular mechanisms distinct from those mediating its proneural activity.

Disciplines :

Anatomy (cytology, histology, embryology...) & physiology
Biochemistry, biophysics & molecular biology

Author, co-author :

Hand, Randal

Bortone, Dante

Mattar, Pierre

Nguyen, Laurent ; Université de Liège - ULiège > Département des sciences cliniques > Neurologie

Heng, Julian Ik-Tsen

Guerrier, Sabrice

Boutt, Elizabeth

Peters, Eldon

Barnes, Anthony P

Parras, Carlos

More authors (3 more)

Language :

English

Title :

Phosphorylation of Neurogenin2 specifies the migration properties and the dendritic morphology of pyramidal neurons in the neocortex.

Publication date :

2005

Journal title :

Neuron

ISSN :

0896-6273

eISSN :

1097-4199

Publisher :

Cell Press, Cambridge, United Kingdom

Volume :

Issue :

Pages :

45-62

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 28 January 2009

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Scopus citations^®

295

Scopus citations^®
without self-citations

221

OpenCitations

283

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