[en] Incubation of rat brain homogenates with thiamine or thiamine diphosphate (TDP) leads to a synthesis of thiamine triphosphate (TTP). In membrane vesicles subsequently prepared from the homogenates, increased TTP content correlates with increased 36Cl- uptake. A hyperbolic relationship was obtained with a K0.5 of 0.27 nmol TTP/mg protein. In crude mitochondrial fractions from the brains of animals previously treated with thiamine or sulbutiamine, a positive correlation between 36Cl- uptake and TTP content was found. These results, together with other results previously obtained with the patch-clamp technique, suggest that TTP is an activator of chloride channels having a large unit conductance.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Bettendorff, Lucien ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
Bettendorff, Hennuy, Wins, Schoffeniels (1993) Thiamine and derivatives as modulators of rat brain chloride channels. Neuroscience 52:1009-1017.
Bettendorff, Kolb, Schoffeniels (1993) Thiamine triphosphate activates an anion channel of large unit conductance in neuroblastoma cells. J. Membrane Biol. 136:281-288.
Bettendorff, Peeters, Jouan, Wins, Schoffeniels (1991) Determination of thiamin and its phosphate esters in cultured neurons and astrocytes using an ion-pair reversed phase high-performance liquid chromatographic method. Anal. Biochem. 198:52-59.
Bettendorff, Peeters, Wins, Schoffeniels (1993) Metabolism of thiamine triphosphate in rat brain: correlation with chloride permeability. J. Neurochem. 60:423-434.
Bettendorff, Weekers, Wins, Schoffeniels (1990) Injection of sulbutiamine induces an increase in thiamine triphosphate in rat tissues. Biochem. Pharmacol. 40:2557-2560.
Bettendorff, Wins, Schoffeniels (1990) Regulation of ion uptake in membrane vesicles from rat brain by thiamine compounds. Biochem. Biophys. Res. Commun. 171:1137-1144.
Blatz, Magleby (1983) Single voltage-dependent chloride-sensitive channels of large conductance in cultured rat muscle. Biophys. J. 43:237-241.
Bolotina, Borecky, Vlachová, Baudysová, Vyskocil (1987) Voltage-dependent chloride channels with several substates in excised patches from mouse neuroblastoma cells. Neuroscience Letters 77:298-302.
Cooper, Pincus (1979) The role of thiamine in the nervous tissue. Neurochem. Res. 4:223-239.
Falke, Misler (1989) Activity of ion channels during volume regulation by clonal N1E115 neuroblastoma cells. Proc. Natl. Acad. Sci. USA 86:3919-3923.
Harris, Allan (1985) Functional coupling of γ-aminobutyric acid receptors to chloride channels in brain membranes. Science 228:1108-1110.
Kolb, Brown, Murer (1985) Identification of a voltage-dependent anion channel in the apical membrane of a Cl−-secretory epithelium (MDCK). Pflügers Arch. 403:262-265.
Mangel, Raymond, Fitz (1993) Regulation of high-conductance anion channels by G proteins and 5-HT1A receptors in CHO cells. Am. J. Physiol. 264:F490-F495.
Nishino, Itokawa, Nishino, Piros, Cooper (1983) Enzyme system involved in the synthesis of thiamin triphosphate. 1. Purification and characterization of protein-bound thiamine diphosphate: ATP phosphoryltransferase. J. Biol. Chem. 258:11871-11878.
Pahapill, Schlichter (1992) Cl− channels in intact human T lymphocytes. J. Membrane Biol. 125:171-183.
Peterson (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal. Biochem. 83:346-356.
Schlichter, Grygorczyk, Pahapill, Gryygorczyk (1990) A large, multiple-conductance chloride channel in normal human T lymphocytes. Pflügers Arch. 416:413-421.
Schwarze, Kolb (1984) Voltage-dependent kinetics of an anionic channel of large unit conductance in macrophages and myotube membranes. Pflügers Arch. 402:281-291.
Soejima, Kokubun (1988) Single anion-selective channel and its ion selectivity in the vascular smooth muscle cell. Pflügers Arch. 411:304-311.
Sun, Supplisson, Torres, Sachs, Mayer (1992) Characterization of large chloride channels in rabbit colonic smooth muscle. J. Physiol. (London) 448:355-382.
Woll, Leibowitz, Neumcke, Hille (1987) A high-conductance anion channel in adult amphibian skeletal muscle. Pflügers Arch. 410:632-640.