[en] Several membrane fractions were prepared from rat brain by differential and sucrose density gradient centrifugation. Most fractions took up 36Cl- rapidly at a rate linear with time during the first 30-60 s, then the rate progressively slowed down. The lowest rate of uptake was found in the mitochondrial fraction. Oxythiamin partially inhibited 36Cl- uptake in all fractions. In P2 (crude synaptosomal fraction), oxythiamin decreased the initial rate of uptake by 32%, the apparent Ki being 1.5 mM. Thiamin and amprolium were less effective as inhibitors. 4,4'-Diisothiocyanostilbene-2,2'-disulfonic acid (0.1-1 mM) inhibited 36Cl- uptake by 40-50%. In the presence of this compound at a concentration > or = 5 x 10(-4) M, oxythiamin became ineffective. 36Cl- uptake was increased by GABA (0.1 mM) and this effect was antagonized by picrotoxin as expected, but not by oxythiamin. The rate of 36Cl- uptake did not appreciably depend on the external chloride concentration and was unaffected by bumetanide or by replacement of external Na+ by choline. Taken together, these data suggest that the oxythiamin-sensitive 36Cl- influx is essentially diffusional and is not related to the GABA receptor or the Na:K:2Cl co-transport. Partial replacement of external Na+ by K+ or treatment with 0.1 mM veratridine (which should both result in membrane depolarization) increased 36Cl- uptake by 50 and 30% respectively; the inhibitory effect of oxythiamin was enhanced to the same proportion.(ABSTRACT TRUNCATED AT 250 WORDS)
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Bettendorff, Lucien ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
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Bibliography
Allan, Harris (1986) γ-Aminobutyric acid agonists and antagonists alter chloride flux across brain membranes. Molec. Pharmac. 29:497-505.
Armett, Cooper (1965) The role of thiamine in nervous tissue: effect of antimetabolites of the vitamin on conduction in mammalian nonmyelinated nerve fiber. J. Pharmac. exp. Ther. 148:137-143.
Bear (1988) Phosphorylation-activated chloride channels in human skin fibroblasts. FEBS Letters 237:145-149.
Bettendorff (1991) Application of high-performance liquid chromatography to the study of thiamine metabolism and in particular thiamine triphosphatase. J. Chromat. 566:397-408.
Bettendorff, Michel-Cahay, Grandfils, De Rycker, Schoffeniels (1987) Thiamine triphosphate and membrane-associated thiamine phosphatases in the electric organ of Electrophorus electricus. J. Neurochem. 49:495-502.
Bettendorff, Peelers, Jouan, Wins, Schoffeniels (1991) Determination of thiamin and its phosphate esters in cultured neurons and astrocytes using an ion-pair reversed phase high-performance liquid Chromatographic method. Analyt. Biochem. 198:52-59.
Bettendorff, Peelers, Wins, Schoffeniels (1993) Metabolism of thiamin triphosphate in rat brain correlation with chloride permeability. J Neurochem, (in press).; .
Bettendorff, Wins, Schoffeniels (1990) Regulation of ion uptake in membrane vesicles from rat brain by thiamine compounds. Biochem. biophys. Res. Commun. 171:1137-1144.
Blatz, Magleby (1983) Single voltage-dependent Cl− selective channels of large conductance in cultured rat muscle. Biophys. J. 43:237-241.
Blatz, Magleby (1985) Single chloride-selective channels active at resting membrane potentials in cultured rat skeletal muscle. Biophys. J. 47:119-123.
Cabantchik, Knauf, Rothstein (1978) The anion transport system of the red blood cell. The role of membrane protein evaluated by use of ‘probes’. Biochim. biophys. Acta 515:239-302.
Cooper, Pincus (1979) The role of thiamine in the nervous tissue. Neurochem. Res. 4:223-239.
Eder, Dunant, Loctin (1980) Thiamine and cholinergic transmission in the electric organ of Torpedo. II. Effects of exogenous thiamine and analogues on acetylcholine release. J. Neurochem. 35:1287-1296.
Eldefrawi, Eldefrawi (1987) Receptors for γ-aminobutyric acid and voltage-dependent chloride channels as drugs and toxicants. Fedn Proc. Fedn Am. Socs exp. Biol. , 3rd edn.; 1:262-271.
Franciolini, Nonner (1987) Anion and cation permeability of a chloride channel in rat hippocampal neurons. J. gen. Physiol. 90:453-478.
Goldberg, Miller (1991) Solubilization and functional reconstitution of a chloride channel from Torpedo californica electroplax. J. Membrane Biol. 124:199-206.
Haas (1989) Properties and diversity of (Na-K-Cl) cotransporters. A. Rev. Physiol. 51:443-457.
Harris, Allan (1985) Functional coupling of γ-aminobutyric acid receptors to chloride channels in brain membranes. Science 228:1108-1110.
Hoffmann (1986) Anion transport systems in the plasma membrane of vertebrate cells. Biochim. biophys. Acta 864:1-31.
Inoue (1985) Voltage-dependent chloride conductance of the squid axon membrane and its blockade by some disulfonic stilbene derivatives. J. gen. Physiol. 85:519-537.
Itokawa, Cooper (1970) Ion movements and thiamine in nervous tissue—I. Intact nerve preparations. Biochem. Pharmac. 19:985-992.
Kimelberg (1981) Active accumulation and exchange transport of chloride in astroglial cells in culture. Biochim. biophys. Acta 646:179-184.
Krueger, Blaustein, Ratzlaff (1980) Sodium channels in presynaptical nerve terminals. Regulation by neurotoxins. J. gen. Physiol. 76:287-313.
Matthews, Albuquerque, Eldefrawi (1979) Influence of batrachotoxin, veratridine, grayanotoxin I and tetrodotoxin on uptake of Na-22 by rat brain membrane preparations. Life Sci. 25:1651-1658.
Muralt (1947) Thiamine in peripheral neurophysiology. Vitamins and Hormones , 3rd edn., R.S. Harris, K.V. Thimann, Academic Press, New York; 5:93-118.
Nelson (1986) Interactions of divalent cations with single calcium channels from rat brain synaptosomes. J. gen. Physiol. 87:201-222.
Nomura, Sokabe (1991) Anion channels from rat brain synaptosomal membranes incorporated into planar bilayer. J. Membrane Biol. 124:53-62.
Nowak, Ascher, Berwald-Netter (1987) Ionic channels in mouse astrocytes in culture. J. Neurosci. 7:101-109.
Palade, Barchi (1977) On the inhibition of muscle membrane chloride conductance by aromatic carboxylic acids. J. gen. Physiol. 69:879-896.
Peterson (1977) A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analyt. Biochem. 83:346-356.
Polin, Wynosky, Porter (1963) In vivo absorption of amprolium and its competition with thiamine. Proc. Soc. exp. Biol. Med. , 3rd edn.; 114:273-277.
Rindi, de Giuseppe, Ventura (1963) Distribution and phosphorylation of oxythiamin in rat tissues. J. Nutr. 81:147-154.
Schoffeniels (1989) Aspects moléculaires de la bioélectrogenése. Archives Of Physiology And Biochemistry 97:387-402.
Schwartz, Susdak, Paul (1986) γ-Aminobutyric acid (GABA)- and barbiturate-mediated36Cl− uptake in rat brain synaptoneurosomes: evidence for rapid desensitization of the GABA receptor-coupled chloride ion channel. Molec. Pharmac. 30:419-426.
Schwarze, Kolb (1984) Voltage-dependent kinetics of an anionic channel of large unit conductance in macrophages and myotube membranes. Pflügers Arch. 402:281-291.
Simchowitz, Ratzlaff, De Weer (1986) Anion/anion exchange in human neutrophils. J. gen. Physiol. 88:195-217.
White (1975) A role of divalent cations in the uptake of noradrenaline by synaptosomes. J. Neurochem. 24:1037-1042.
White, Miller (1979) A voltage-gated anion channel from the electric organ of Torpedo californica. J. biol. Chem. 254:10161-10166.
White, Miller (1981) Probes of the conduction process of a voltage-gated Cl channel from Torpedo electroplax. The Journal of General Physiology 78:1-18.
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