No full text
Article (Scientific journals)
Thiamin and Derivatives as Modulators of Rat Brain Chloride Channels
Bettendorff, Lucien; Hennuy, Benoît; Wins, Pierre et al.
1993In Neuroscience, 52 (4), p. 1009-1017
Peer Reviewed verified by ORBi
 

Files


Full Text
No document available.

Send to



Details



Abstract :
[en] Several membrane fractions were prepared from rat brain by differential and sucrose density gradient centrifugation. Most fractions took up 36Cl- rapidly at a rate linear with time during the first 30-60 s, then the rate progressively slowed down. The lowest rate of uptake was found in the mitochondrial fraction. Oxythiamin partially inhibited 36Cl- uptake in all fractions. In P2 (crude synaptosomal fraction), oxythiamin decreased the initial rate of uptake by 32%, the apparent Ki being 1.5 mM. Thiamin and amprolium were less effective as inhibitors. 4,4'-Diisothiocyanostilbene-2,2'-disulfonic acid (0.1-1 mM) inhibited 36Cl- uptake by 40-50%. In the presence of this compound at a concentration > or = 5 x 10(-4) M, oxythiamin became ineffective. 36Cl- uptake was increased by GABA (0.1 mM) and this effect was antagonized by picrotoxin as expected, but not by oxythiamin. The rate of 36Cl- uptake did not appreciably depend on the external chloride concentration and was unaffected by bumetanide or by replacement of external Na+ by choline. Taken together, these data suggest that the oxythiamin-sensitive 36Cl- influx is essentially diffusional and is not related to the GABA receptor or the Na:K:2Cl co-transport. Partial replacement of external Na+ by K+ or treatment with 0.1 mM veratridine (which should both result in membrane depolarization) increased 36Cl- uptake by 50 and 30% respectively; the inhibitory effect of oxythiamin was enhanced to the same proportion.(ABSTRACT TRUNCATED AT 250 WORDS)
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Bettendorff, Lucien  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique
Hennuy, Benoît ;  Université de Liège - ULiège > GIGA-Management : Plate-forme transcriptomique
Wins, Pierre
Schoffeniels, Ernest
Language :
English
Title :
Thiamin and Derivatives as Modulators of Rat Brain Chloride Channels
Publication date :
1993
Journal title :
Neuroscience
ISSN :
0306-4522
eISSN :
1873-7544
Publisher :
Elsevier, Netherlands
Volume :
52
Issue :
4
Pages :
1009-1017
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 23 January 2009

Statistics


Number of views
47 (7 by ULiège)
Number of downloads
0 (0 by ULiège)

Scopus citations®
 
22
Scopus citations®
without self-citations
14
OpenCitations
 
18

Bibliography


Similar publications



Contact ORBi