Reference : Regulation of Ion Uptake in Membrane Vesicles from Rat Brain by Thiamine Compounds
Scientific journals : Article
Life sciences : Biochemistry, biophysics & molecular biology
http://hdl.handle.net/2268/4576
Regulation of Ion Uptake in Membrane Vesicles from Rat Brain by Thiamine Compounds
English
Bettendorff, Lucien mailto [Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biochimie et physiologie humaine et pathologique >]
Wins, Pierre [> > > >]
Schoffeniels, Ernest [> > > >]
1990
Biochemical and Biophysical Research Communications
171
3
1137-1144
Yes (verified by ORBi)
International
0006-291X
[en] We examined the effects of thiamine derivatives on ion uptake in rat brain membrane vesicles. Thiamine triphosphate (1 mM) and pyrithiamine (0.1 mM) increase chloride uptake. Preincubation of crude homogenate with thiamine or pyrithiamine increases chloride uptake while oxythiamine has the reverse effect. Thiamine and oxythiamine also affect 22Na+ and 86Rb+ uptake in the same way as for 36Cl- but to a lesser extent. Thiamine-dependent 36Cl- uptake is activated by sodium bicarbonate (10 mM) and partially inhibited by bumetanide (0.1 mM) and 2,4-dinitrophenol (0.1 mM). Preincubation with thiamine increases the thiamine triphosphate content of the vesicles. The hypothesis that TTP is the activator of a particular chloride uptake mechanism is discussed.
http://hdl.handle.net/2268/4576
10.1016/0006-291X(90)90803-U

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