[en] Detection of cattle persistently infected with bovine viral diarrhoea virus (BVDV) is crucial to controlling mucosal disease. A sandwich enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies raised against the 48-kDa glycoprotein and the 120/80-kDa protein was developed for detecting antigens in leucocytes of 3 persistently BVDV-infected calves. The test is simple, sensitive and rapid. Moreover the same ELISA was able to recognise Belgian field isolates of BVDV. These results show that the test can be applied in the field.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Mignon, Bernard ; Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie - Immunologie
Dubuisson, J.
Baranowski, E.
Koromyslov, I.
Ernst, E.; Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie - Immunologie
Boulanger, D.; Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie - Immunologie
Waxweiler, Sophie; Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie - Immunologie
Pastoret, Paul-Pierre ; Université de Liège - ULiège > Faculté de médecine vétérinaire > Virologie - Immunologie
Language :
English
Title :
A Monoclonal Elisa for Bovine Viral Diarrhoea Pestivirus Antigen Detection in Persistently Infected Cattle
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