Epidemiological Typing of Extended-Spectrum Beta-Lactamase-Producing Klebsiella Pneumoniae Isolates by Pulsed-Field Gel Electrophoresis and Antibiotic Susceptibility Patterns
Chetoui, H.; Delhalle, E.; Melin, Pierretteet al.
1999 • In Research in Microbiology, 150 (4), p. 265-72
[en] Over a 16-month period, Klebsiella pneumoniae isolates from 102 patients admitted to a university hospital in Liege (Belgium) produced extended-spectrum beta-lactamases. Pulsed-field gel electrophoresis of genome macrorestriction patterns with XbaI and antibiotic susceptibility patterns subdivided 39 isolates into eight clonally related groups. Two of them were implicated in the course of this outbreak. They were responsible for successive waves of infection or colonization in different wards of the hospital while the others were encountered sporadically. A beta-lactamase with an isoelectric point of 7.6 and consistent with type SHV-2 characterized all nine isolates chosen among both major groups.
Melin, Pierrette ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale
Sabri, Ahmed ; Université de Liège - ULiège > Centre Wallon de biologie industrielle
Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles
De Mol, Patrick ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale
Language :
English
Title :
Epidemiological Typing of Extended-Spectrum Beta-Lactamase-Producing Klebsiella Pneumoniae Isolates by Pulsed-Field Gel Electrophoresis and Antibiotic Susceptibility Patterns
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