Epidemiological Typing of Extended-Spectrum Beta-Lactamase-Producing Klebsiella Pneumoniae Isolates by Pulsed-Field Gel Electrophoresis and Antibiotic Susceptibility Patterns

Chetoui, H.; Delhalle, E.; Melin, Pierrette; Sabri, Ahmed; Thonart, Philippe; De Mol, Patrick

doi:10.1016/S0923-2508(99)80051-5

Article (Scientific journals)

Epidemiological Typing of Extended-Spectrum Beta-Lactamase-Producing Klebsiella Pneumoniae Isolates by Pulsed-Field Gel Electrophoresis and Antibiotic Susceptibility Patterns

Chetoui, H.; Delhalle, E.; Melin, Pierrette et al.

1999 • In Research in Microbiology, 150 (4), p. 265-72

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/4438

DOI
10.1016/S0923-2508(99)80051-5

PubMed
10376488

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Keywords :

Klebsiella pneumoniae; extended-spectrum â-lactamase; macrorestriction typing; antibiotyping; nosocomial infection; epidemiology

Abstract :

[en] Over a 16-month period, Klebsiella pneumoniae isolates from 102 patients admitted to a university hospital in Liege (Belgium) produced extended-spectrum beta-lactamases. Pulsed-field gel electrophoresis of genome macrorestriction patterns with XbaI and antibiotic susceptibility patterns subdivided 39 isolates into eight clonally related groups. Two of them were implicated in the course of this outbreak. They were responsible for successive waves of infection or colonization in different wards of the hospital while the others were encountered sporadically. A beta-lactamase with an isoelectric point of 7.6 and consistent with type SHV-2 characterized all nine isolates chosen among both major groups.

Disciplines :

Biochemistry, biophysics & molecular biology
Microbiology

Author, co-author :

Chetoui, H.

Delhalle, E.

Melin, Pierrette ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale

Sabri, Ahmed ; Université de Liège - ULiège > Centre Wallon de biologie industrielle

Thonart, Philippe ; Université de Liège - ULiège > Département des sciences de la vie > Biochimie et microbiologie industrielles

De Mol, Patrick ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Microbiologie médicale et virologie médicale

Language :

English

Title :

Epidemiological Typing of Extended-Spectrum Beta-Lactamase-Producing Klebsiella Pneumoniae Isolates by Pulsed-Field Gel Electrophoresis and Antibiotic Susceptibility Patterns

Publication date :

May 1999

Journal title :

Research in Microbiology

ISSN :

0923-2508

eISSN :

1769-7123

Publisher :

Elsevier, Netherlands

Volume :

150

Issue :

Pages :

265-72

Peer reviewed :

Peer Reviewed verified by ORBi

Available on ORBi :

since 15 January 2010

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