Article (Scientific journals)
Optimization of the LC enantioseparation of chiral pharmaceuticals using cellulose tris(4-chloro-3-methylphenylcarbamate) as chiral selector and polar non-aqueous mobile phases
Dossou, Katina Sourou Sylvestre; Chiap, Patrice; Chankvetadze, Bezhan et al.
2010In Journal of Separation Science, 33, p. 1699-1707
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Keywords :
Chiral separations; Experimental design; Optimisation; Polar organic solvent chromatography; Polysaccharide-based chiral stationary phase
Abstract :
[en] The resolving power of a new commercial polysaccharide-based chiral stationary phase, Sepapak-4, with cellulose tris(4-chloro-3-methylphenylcarbamate) coated on silica microparticles as chiral selector, was evaluated toward the enantioseparation of ten basic drugs with widely different structures and hydrophobic properties, using ACN as the main component of the mobile phase. A multivariate approach (experimental design) was used to screen the factors (temperature, n-hexane content, acidic and basic additives) likely to influence enantioresolution. Then, the optimization was performed using a face-centered central composite design. Complete enantioseparation could be obtained for almost all tested chiral compounds, demonstrating the high chiral discrimination ability of this chiral stationary phase using polar organic mobile phases made up of ACN and containing an acidic additive (TFA or formic acid), 0.1% diethylamine and n-hexane. These results clearly illustrate the key role of the nature of the acidic additive in the mobile phase.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Dossou, Katina Sourou Sylvestre ;  Université de Liège - ULiège > Doct. sc. bioméd. & pharma. (Bologne)
Chiap, Patrice ;  Université de Liège - ULiège > Pharmacologie clinique
Chankvetadze, Bezhan;  University of Munster
Servais, Anne-Catherine  ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Fillet, Marianne ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Crommen, Jacques ;  Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Optimization of the LC enantioseparation of chiral pharmaceuticals using cellulose tris(4-chloro-3-methylphenylcarbamate) as chiral selector and polar non-aqueous mobile phases
Publication date :
2010
Journal title :
Journal of Separation Science
ISSN :
1615-9306
eISSN :
1615-9314
Publisher :
Wiley-VCH Verlag Gmbh, Weinheim, Germany
Volume :
33
Pages :
1699-1707
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
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