Association of two single-isomer anionic CD in NACE for the chiral and achiral separation of fenbendazole, its sulphoxide and sulphone metabolites: Application to their determination after in vitro metabolism
[en] A NACE method was developed for the separation of fenbendazole (FBZ), a prochiral
drug giving rise to chiral (oxfendazole or OFZ) and nonchiral (FBZ sulphone or FBZSO2)
metabolites. First, the effect of the nature and the concentration of CD as well as that of
the acidic BGE on the enantiomeric separation of OFZ were studied. OFZ enantiomers
were completely resolved using a BGE made up of 10 mM ammonium formate and
0.5 M TFA in methanol containing 10 mM heptakis(2,3-di-O-acetyl-6-O-sulfo)-beta-CD and
10 mM heptakis(2,3-di-O-methyl-6-O-sulfo)-beta-CD. Moreover, the NACE method was
found to be particularly well suited to the simultaneous determination of FBZ, OFZ
enantiomers, and FBZSO2. Thiabendazole was selected as an internal standard. The
CD-NACE potential was then evaluated for in vitro metabolism studies using FBZ as a
model case. The OFZ enantiomers and FBZSO2 could be detected after incubation of
FBZ in the phenobarbital-induced male rat liver microsomes systems.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Rousseau, Anne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Gillotin, Florian; Université de Liège - ULiège > Pharmacologie clinique
Crommen, Jacques ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Fillet, Marianne ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Servais, Anne-Catherine ; Université de Liège - ULiège > Département de pharmacie > Analyse des médicaments
Language :
English
Title :
Association of two single-isomer anionic CD in NACE for the chiral and achiral separation of fenbendazole, its sulphoxide and sulphone metabolites: Application to their determination after in vitro metabolism
Publication date :
2010
Journal title :
Electrophoresis
ISSN :
0173-0835
eISSN :
1522-2683
Publisher :
Vch Publishers, Weinheim, Germany
Volume :
31
Pages :
1482-1487
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique Fonds Léon Fredericq
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