[en] Neospora caninum is a protozoan parasite, which causes fetal and neonatal mortality in livestock and companion animals. In 224 abortions in Belgian cattle, different diagnostic methods were used to demonstrate infection, and the presence of N. caninum. An indirect fluorescent antibody test (IFAT) was used to analyze fetal and maternal sera and immunohistochemistry (IHC) was performed when lesions consistent with neosporosis were observed in the brain, heart or liver. Twenty dairy cattle sera out of 70 (29%) and 13 beef cattle sera out of 93 (14%) were positive by IFAT. A positive titer to N. caninum was found in seven and three fetuses born to beef and dairy cows, respectively. Lesions consistent with N. caninum infection were observed in 17 fetuses. Of nine positive beef fetuses, five were confirmed by IHC while, all but one dairy fetus were confirmed using the same technique. Age had no influence on the serological status of the mother (P = 0.486) whereas husbandry system had a borderline influence (P = 0.082). However, a strong association (P = 0.004) between the level of antibodies in the darn and the occurrence of lesions in the fetus was observed and lesions were more prominent in dairy than in beef fetuses. Additionally, the distribution of intra-cerebral lesions was more extensive in dairy than in beef fetuses (P < 0.0001). Age and serological status of the fetus were found to influence the occurrence of lesions in beef fetuses (both P < 0.001) but no such significant relationships could be demonstrated in dairy fetuses. The study indicated that N. caninum must be considered as an important cause of bovine abortion in Belgium. (C) 2002 Elsevier Science Inc. All rights reserved.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
De Meerschman, F.
Speybroeck, N.
Berkvens, D.
Rettigner, C.
Focant, Charles ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire
Cassart, Dominique ; Université de Liège - ULiège > Département de morphologie et pathologie > Département de morphologie et pathologie
Losson, Bertrand ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires > Parasitologie et pathologie des maladies parasitaires
Language :
English
Title :
Fetal infection with Neospora caninum in dairy and beef cattle in Belgium
Publication date :
September 2002
Journal title :
Theriogenology
ISSN :
0093-691X
eISSN :
1879-3231
Publisher :
Elsevier Science Inc, New York, United States - New York
scite shows how a scientific paper has been cited by providing the context of the citation, a classification describing whether it supports, mentions, or contrasts the cited claim, and a label indicating in which section the citation was made.
Bibliography
Dubey J.P., Lindsay D.S. (1996) A review of Neospora caninum and neosporosis. Vet Parasitol 67:1-59.
McAllister M.M., Dubey J.P., Lindsay D.S., Jolley W.R., Wills R.A., McGuire A.M. (1998) Dogs are definitive hosts of Neospora caninum. Int J Parasitol 28:1473-1478.
Dubey J.P. (1986) A review of toxoplasmosis in cattle. Vet Parasitol 22:177-202.
Ellis J.T. (1998) Polymerase chain reaction approaches for the detection of Neospora caninum and Toxoplasma gondii. Int J Parasitol 28:1053-1060.
Gottstein B., Hentrich B., Wyss R., Thur B., Busato A., Stark K.D. (1998) Molecular and immunodiagnostic investigations on bovine neosporosis in Switzerland. Int J Parasitol 28:679-691.
Lindsay D.S., Dubey J.P. (1989) Immunohistochemical diagnosis of Neospora caninum in tissue sections. Am J Vet Res 50:1981-1983.
Wouda W., Moen A.R., Visser I.J., Van Knapen F. (1997) Bovine fetal neosporosis: A comparison of epizootic and sporadic abortion cases and different age classes with regard to lesion severity and immunohistochemical identification of organisms in brain, heart, and liver. J Vet Diagn Invest 9:180-185.
Anderson M.L., Blanchard P.C., Barr B.C., Dubey J.P., Hoffman R.L., Conrad P.A. (1991) Neospora-like protozoan infection as a major cause of abortion in California dairy cattle. J Am Vet Med Assoc 198:241-244.
Barr B.C., Anderson M.L., Dubey J.P., Conrad P.A. (1991) Neospora-like protozoal infections associated with bovine abortions. Vet Pathol 28:110-116.
Nietfeld J.C., Dubey J.P., Anderson M.L., Libal M.C., Yaeger M.J., Neiger R.D. (1992) Neospora-like protozoan infection as a cause of abortion in dairy cattle. J Vet Diagn Invest 4:223-226.
Conrad P.A., Sverlow K., Anderson M., Rowe J., BonDurant R., Tuter G. (1993) Detection of serum antibody responses in cattle with natural or experimental Neospora infections. J Vet Diagn Invest 5:572-578.
Pare J., Hietala S.K., Thurmond M.C. (1995) Interpretation of an indirect fluorescent antibody test for diagnosis of Neospora sp. infection in cattle. J Vet Diagn Invest 7:273-275.
Dubey J.P., Jenkins M.C., Adams D.S., McAllister M.M., Anderson R., Baszler T.V. (1997) Antibody responses of cows during an outbreak of neosporosis evaluated by indirect fluorescent antibody test and different enzyme-linked immunosorbent assays. J Parasitol 83:1063-1069.
Lally N.C., Jenkins M.C., Dubey J.P. (1996) Evaluation of two Neospora caninum recombinant antigens for use in an enzyme-linked immunosorbent assay for the diagnosis of bovine neosporosis. Clin Diagn Lab Immunol 3:275-279.
Williams D.J., McGarry J., Guy F., Barber J., Trees A.J. (1997) Novel ELISA for detection of Neospora-specific antibodies in cattle. Vet Rec 140:328-331.
Romand S., Thulliez P., Dubey J.P. (1998) Direct agglutination test for serologic diagnosis of Neospora caninum infection. Parasitol Res 84:50-53.
Schares G., Peters M., Wurm R., Barwald A., Conraths F.J. (1998) The efficiency of vertical transmission of Neospora caninum in dairy cattle analysed by serological techniques. Vet Parasitol 80:87-98.
Schares G., Conraths F.J., Reichel M.P. (1999) Bovine neosporosis: Comparison of serological methods using outbreak sera from a dairy herd in New Zealand. Int J Parasitol 29:1659-1667.
McAllister M.M., Huffman E.M., Hietala S.K., Conrad P.A., Anderson M.L., Salman M.D. (1996) Evidence suggesting a point source exposure in an outbreak of bovine abortion due to neosporosis. J Vet Diagn Invest 8:355-357.
De Meerschman F., Czaplicki G., Focant C., Boreux R., Leclipteux T., Losson B. (2000) Cattle neosporosis in Belgium: A case-control study in dairy and beef cattle. Int J Parasitol , Hemphill A, Gottstein B, editors. A European perspective on Neospora caninum; 30:877-924.
Waldner C.L., Janzen E.D., Ribble C.S. (1998) Determination of the association between Neospora caninum infection and reproductive performance in beef herds. J Am Vet Med Assoc 213:685-690.
Waldner C.L., Janzen E.D., Henderson J., Haines D.M. (1999) Outbreak of abortion associated with Neospora caninum infection in a beef herd. J Am Vet Med Assoc 215:1485-1489.
Quintanilla-Gozalo A., Pereira-Bueno J., Tabares E., Innes E.A., Gonzalez-Paniello R., Ortega-Mora L.M. (1999) Seroprevalence of Neospora caninum infection in dairy and beef cattle in Spain. Int J Parasitol 29:1201-1208.
Venturini M.C., Venturini L., Bacigalupe D., Machuca M., Echaide I., Basso W. (1999) Neospora caninum infections in bovine fetuses and dairy cows with abortions in Argentina. Int J Parasitol 29:1705-1708.
Wouda W., Dubey J.P., Jenkins M.C. (1997) Serological diagnosis of bovine fetal neosporosis. J Parasitol 83:545-547.
Mehta C.R., Patel N.R. (1995) Exact logistic regression: Theory and examples. Stat Med 14:2143-2160.
Cicchetti D.V., Feinstein A.R. (1990) High agreement but low kappa: II. Resolving the paradoxes. J Clin Epidemiol 43:551-558.
Graham P., Bull B. (1998) Approximate standard errors and confidence intervals for indices of positive and negative agreement. J Clin Epidemiol 51:763-771.
Ihaka R., Gentleman R.R. (1996) A language for data analysis and graphics. J Comput Graphics Stat 5:299-314.
Stata Statistical Software: Release 7.0, College Station, TX: Stata Corporation; 2001.
Barr B.C., Anderson M.L., Blanchard P.C., Daft B.M., Kinde H., Conrad P.A. (1990) Bovine fetal encephalitis and myocarditis associated with protozoal infections. Vet Pathol 27:354-361.
Otter A., Jeffrey M., Griffiths I.B., Dubey J.P. (1995) A survey of the incidence of Neospora caninum infection in aborted and stillborn bovine fetuses in England and Wales. Vet Rec 136:602-606.
Ogino H., Watanabe E., Watanabe S., Agawa H., Narita M., Haritani M. (1992) Neosporosis in the aborted fetus and newborn calf. J Comp Pathol 107:231-237.
Williams D.J., Guy C.S., Mc Garry J.W., Guy F., Tasker L., Smith R.F. (2000) Neospora caninum-associated abortion in cattle: The time of experimentally-induced parasitaemia during gestation determines foetal survival. Parasitology 121:347-358.
Thurmond M.C., Hietala S.K., Blanchard P.C. (1999) Predictive values of fetal histopathology and immunoperoxidase staining in diagnosing bovine abortion caused by Neospora caninum in a dairy herd. J Vet Diagn Invest 11:90-94.
Gonzalez L., Buxton D., Atxaerandio R., Aduriz G., Maley S., Marco J.C. (1999) Bovine abortion associated with Neospora caninum in northern Spain. Vet Rec 144:145-150.
Davison H.C., French N.P., Trees A.J. (1999) Herd-specific and age-specific seroprevalence of Neospora caninum in 14 British dairy herds. Vet Rec 144:547-550.
Dijkstra T., Barkema H.W., Eysker M., Wouda W. (2001) Evidence of post-natal transmission of Neospora caninum in Dutch dairy herds. Int J Parasitol 31:209-215.
Dyer R.M., Jenkins M.C., Kwok O.C., Douglas L.W., Dubey J.P. (2000) Serologic survey of Neospora caninum infection in a closed dairy cattle herd in Maryland: Risk of serologic reactivity by production groups. Vet Parasitol 90:171-181.
Davison H.C., Otter A., Trees A.J. (1999) Estimation of vertical and horizontal transmission parameters of Neospora caninum infections in dairy cattle. Int J Parasitol 29:1683-1689.
Hietala S.K., Thurmond M.C. (1999) Postnatal Neospora caninum transmission and transient serologic responses in two dairies. Int J Parasitol 29:1669-1676.
Thurmond M.C., Hietala S.K. (1997) Effect of congenitally acquired Neospora caninum infection on risk of abortion and subsequent abortions in dairy cattle. Am J Vet Res 58:1381-1385.
Jenkins M.C., Wouda W., Dubey J.P. (1997) Serological response over time to recombinant Neospora caninum antigens in cattle after a neosporosis-induced abortion. Clin Diagn Lab Immunol 4:270-274.
Quintanilla-Gozalo A., Pereira-Bueno J., Seijas-Carballedo A., Costas E., Ortega-Mora L.M. (2000) Observational studies in Neospora caninum infected dairy cattle: Relationship infection-abortion and gestational antibody fluctuations. Int J Parasitol , Hemphill A, Gottstein B, editors. A European perspective on Neospora caninum; 30:877-924.
Stenlund S., Kindahl H., Magnusson U., Uggla A., Bjorkman C. (1999) Serum antibody profile and reproductive performance during two consecutive pregnancies of cows naturally infected with Neospora caninum. Vet Parasitol 85:227-234.
Barling K.S., McNeill J.W., Thompson J.A., Paschal J.C., McCollum F.T., Craig T.M. (2000) Association of serologic status for Neospora caninum with postweaning weight gain and carcass measurements in beef calves. J Am Vet Med Assoc 217:1356-1360.
Klein F., Hietala S.K., Berthet H., Very P. (1997) Neospora caninum: Enquête sérologique sur les avortements des bovins normands et charolais. Point Vét 28:1283-1286.
Similar publications
Sorry the service is unavailable at the moment. Please try again later.
This website uses cookies to improve user experience. Read more
Save & Close
Accept all
Decline all
Show detailsHide details
Cookie declaration
About cookies
Strictly necessary
Performance
Strictly necessary cookies allow core website functionality such as user login and account management. The website cannot be used properly without strictly necessary cookies.
This cookie is used by Cookie-Script.com service to remember visitor cookie consent preferences. It is necessary for Cookie-Script.com cookie banner to work properly.
Performance cookies are used to see how visitors use the website, eg. analytics cookies. Those cookies cannot be used to directly identify a certain visitor.
Used to store the attribution information, the referrer initially used to visit the website
Cookies are small text files that are placed on your computer by websites that you visit. Websites use cookies to help users navigate efficiently and perform certain functions. Cookies that are required for the website to operate properly are allowed to be set without your permission. All other cookies need to be approved before they can be set in the browser.
You can change your consent to cookie usage at any time on our Privacy Policy page.
