Article (Scientific journals)
HIV-1 promoter activation following an oxidative stress mediated by singlet oxygen
Legrand, Sylvie; Hoebeke, Maryse; Vaira, Dolorès et al.
1993In Journal of Photochemistry and Photobiology B: Biology, 17 (3), p. 229-237
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Keywords :
HIV-1; Singlet oxygen; Promoter activation
Abstract :
[en] Various biological processes, such as photosensitization or inflammatory reactions, can generate singlet oxygen (O-1(2)) as one of the major oxidative species. Because this oxidant can be generated either extracellularly or intracellularly, it can cause severe damage to various biological macromolecules, even to those deeply embedded inside the cells such as DNA. Sublethal biological modifications induced by different DNA-damaging agents can promote various cellular responses initiated by the activation of various cellular genes and certain heterologous viruses. Since O-1(2) fulfils essential prerequisites for a genotoxic substance, we have examined the effects of an oxidative stress, mediated by this species, on cells harbouring a heterologous promoter-leader sequence derived from the human immunodeficiency virus type 1 (HIV-1). Our results demonstrate that HIV-1 long terminal repeat (LTR), integrated into the cellular I)NA of epithelial cells, can be transactivated following an oxidative stress mediated by O-1(2). In addition, using HIV-1 latently infected promonocytes or lymphocytes, it can be shown that virus reactivation can be induced through a sublethal dose of O-1(2) generated intracellularly. An extracellular generation of O-1(2) can promote a substantial lethal effect without HIV-1 reactivation. These data may be relevant to the understanding of the events converting a latent infection into a productive one and to the appearance of the acquired immune deficiency syndrome.
Disciplines :
Immunology & infectious disease
Author, co-author :
Legrand, Sylvie ;  Université de Liège - ULiège > Virologie - Immunologie
Hoebeke, Maryse  ;  Université de Liège - ULiège > Département de physique > Spectroscopie biomédicale
Vaira, Dolorès ;  Centre Hospitalier Universitaire de Liège - CHU > Immuno-hématologie
Rentier, Bernard  ;  Université de Liège - ULiège > Département de Microbiologie > Virologie fondamentale et Immunologie
Piette, Jacques ;  Université de Liège - ULiège > Département des sciences de la vie > Virologie - Immunologie
Language :
Title :
HIV-1 promoter activation following an oxidative stress mediated by singlet oxygen
Publication date :
March 1993
Journal title :
Journal of Photochemistry and Photobiology B: Biology
Publisher :
Volume :
Issue :
Pages :
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
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