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[en] Thanks to their photosynthetic and nutritive properties, cyanobacteria of the Arthrospira genus are of interest as food supplements, as efficient oxygen producing life support system organisms for manned space flight, and for the production of biofuels. Despite these potential valuable applications, full genome sequences and genetic information in general on Arthrospira remain scarce. This is mainly due to the difficulty to extract sufficient high molecular weight nucleic acids from these filamentous cyanobacteria.
In this article, an efficient and reproducible DNA extraction procedure for cyanobacteria of the genus Arthrospira was developed. The method is based on the combination of a soft mechanical lysis with enzymatic disruption of the cell wall. The comparison with other extraction protocols clearly indicates that this optimised method allows the recovery of a larger amount of DNA. Furthermore, the extracted DNA presents a high molecular weight, a reduced degradation and an excellent overall quality. It can be directly used for molecular biology purposes such as PCR, and clone library construction.
Research Center/Unit :
CIP - Centre d'Ingénierie des Protéines - ULiège
Disciplines :
Microbiology
Author, co-author :
Morin, Nicolas ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Vallaeys, Tatiana
Hendrickx, Larissa
Leys, Natalie
Wilmotte, Annick ; Université de Liège - ULiège > Département des sciences de la vie > Physiologie et génétique bactériennes
Language :
English
Title :
An efficient DNA isolation protocol for filamentous cyanobacteria of the genus Arthrospira
Alternative titles :
[fr] Un protocole d'isolement d'ADN efficace pour les cyanobactéries filamenteuses du genre Arthrospira
Publication date :
2010
Journal title :
Journal of Microbiological Methods
ISSN :
0167-7012
eISSN :
1872-8359
Publisher :
Elsevier Science, Amsterdam, Netherlands
Volume :
80
Pages :
148-154
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Studies on the response to spaceflight related conditions in the cyanobacterium Arthrospira sp. PCC8005 using a genomic approach
Funders :
StudieCentrum voor Kernenergie/Centre d'Etudes Nucléaires (SCK-CEN), Mol, Belgie ASE - Agence Spatiale Européenne
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