[en] It has been proposed that among the various cell-surface proteins capable of interacting with laminin, the 67-kd high-affinity laminin receptor plays a crucial role during tumor invasion and metastasis. In this study, the expression of laminin-receptor-precursor messenger RNA (mRNA) and 67-kd protein was analyzed in human colon adenocarcinoma. In 22 of 23 patients with colon cancer, we found a 2- to 23-fold increase in levels of laminin-receptor-precursor mRNA in the cancer tissues compared with those in matched normal adjacent colonic mucosa. In 10 of 11 cases studied, the level of 67-kd laminin receptor, detected by affinity-purified anti-laminin-receptor synthetic peptide antibodies on immunoblots of matched tumor and normal tissue extracts, was higher in the colon carcinoma tissue. Immunodetection of laminin receptor in tissue sections using anti-laminin-receptor-peptide antibodies confirmed that the increased expression of laminin receptor was specifically associated with the cancer cells. In a series of 72 paraffin sections of colon lesions, we observed a correlation between the expression of the laminin receptor and the Dukes' classification. Our observations indicate that increased expression of laminin-receptor-precursor mRNA is associated with enhanced levels of the 67-kd laminin receptor as well as with the invasive phenotype of colon carcinoma. Detection of this metastasis-associated gene product may be a valuable adjunct in the evaluation of human colon cancer.
Castronovo, Vincenzo ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases
Shmookler, B. M.
Garbisa, S.
Grigioni, W. F.
Liotta, L. A.
Sobel, M. E.
Language :
English
Title :
Increased expression of the laminin receptor in human colon cancer.
Publication date :
1991
Journal title :
Journal of the National Cancer Institute
ISSN :
0027-8874
eISSN :
1460-2105
Publisher :
Oxford University Press, Cary, United States - North Carolina
Silverberg E, Boring CC, Squires TS: Cancer statistics 1990. CA 40:9-25, 1990
Silverberg E, Boring CC, Squires TS: Cancer statistics 1990. CA 40:9-25, 1990
Fearon ER. Vogelstein B: A genetic model for colorectal tumorigenesis. Cell 61:759-767, 1990
Cohen AM, Shank B, Friedman MA: Colorectal cancer. In Cancer: Principles and Practice of Oncology (DeVita VT Jr, Heilman S, Rosenberg SA, eds). Philadelphia: Lippincott, 1989, pp 896-964
Liotta LA, Rao CN, Barsky SH: Tumor invasion and the extracellular matrix. Lab Invest 49:639-649, 1983
Poste G, Fidler I: The pathogenesis of cancer metastases. Nature 283:139-146, 1980
Timpl R, Rhode H, Robey PG, et al: Laminin: A glycoprotein from basement membranes. J Biol Chem 254:9933-9941, 1979
Kleinman HK, Cannon FB, Laurie GW, et al: Biological activities of laminin. Cell Biochem 27:317-325, 1985
Barsky SH, Rao CN, Williams JE, et al: Domains oflaminin which alter metastases in a murine model. J Clin Invest 74:843-848, 1984
Varani J, Lovett EJ, McCoy JP, et al: Differential expression of a laminin-like substance by high and low metastatic tumor cells. Am J Pathol 111:27-34, 1983
Terranova VP, Liotta LA, Russo RG, et al: Role of laminin in the attachment and metastasis of murine tumor cells. Cancer Res 42:2265-2273, 1982
Terranova VP, Rao CN, Kalebic T, et al: Laminin receptor on human breast carcinoma cells. Proc Natl Acad Sci USA 80:444-451, 1983
Horwitz A, Duggan K, Greggs R, et al: The cell substrate attachment (CSAT) antigen has properties of a receptor for laminin and fibronectin. J Cell Biol 101:2134-2144, 1985
Kleinman HK, Ogle RC, Cannon FB, et al: Laminin receptors for neurite formation. Proc Natl Acad Sci USA 85:1282-1286, 1988
Gehlsen KR, Dillner L, Engvall E, et al: The human laminin receptor is a member of the integrin family of cell adhesion receptors. Science 241:1228-1229, 1988
Kramer RH, McDonald KA, Vu MP: Human melanoma cells express a novel integrin receptor for laminin. J Biol Chem 264:15642-15649, 1989
Rao CN, Barsky SH, Terranova VP, et al: Isolation of a tumor cell laminin receptor. Biochem Biophys Res Commun 111:804-808, 1983
Malinoff H, Wicha MS: Isolation of a cell surface receptor protein for laminin from murine fibrosarcoma cells. J Cell Biol 96:1475-1480, 1983
Hand PH, Thor A, Schlom J, et al: Expression oflaminin receptor in normal and carcinomatous tissues as defined by a monoclonal antibody. Cancer Res 45:2713-2719, 1985
Wewer UM, Liotta LA, Jaye M, et al: Altered levels oflaminin receptor mRNA in various human carcinoma cells that have different abilities to bind laminin. Proc Natl Acad Sci USA 83:7137-7141, 1986
Rao CN, Castronovo V, Schmitt MC, et al: Evidence for a precursor of the high-affinity metastasis-associated murine laminin receptor. Biochemistry 28:7476-7486, 1989
Yow H, Wong JM, Chen HS, et al: Increased mRNA expression of a laminin-binding protein in human colon carcinomas: Complete sequence of a full-length cDNA encoding the protein. Proc Natl Acad Sci USA 85:6394-6398, 1988
Lesot H, Kuhl U, von der Mark K: Isolation of a laminin binding protein from muscle cell membranes. EMBOJ 2:861-869, 1983
Castronovo V, Taraboletti G, Liotta LA, et al: Modulation oflaminin receptor expression by estrogen and progestins in human breast cancer cell lines. J Natl Cancer Inst 81:781-786, 1989
Castronovo V, Colin C, Claysmith AP, et al: Immunodetection of the metastasis-associated laminin receptor in human breast cancer cells obtained by fine needle aspiration biopsy. Am J Pathol 137:1373-1381, 1990
Cleveland DW, Lopata MA, MacDonald RJ, et al: Number and evolutionär)' conservation of alpha- and beta-tubulin and cytoplasmic beta- and gamma-actin genes using specific cloned DNA probes. Cell 20:95-105, 1980
Hsu SM, Raine L, Fänger H: The use ofavidin-biotin-peroxidase complex (ABC) in immunoperoxidase technique: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577-580, 1981
Castronovo V, Sobel ME: Laminin and fibronectin increase the steady state level of the 67 kd high affinity metastasis-associated laminin receptor mRNA in human cancer cells. Biochem Biophys Res Commun 168:1110-1117, 1990
Mafune K, Ravikumar TS, Wong JM, et al: Expression of a Mr 32, 000 laminin-binding protein messenger RNA in human colon carcinoma correlates with disease progression. Cancer Res 50:3888-3891, 1990
Rahman A, Panneerselvam M, Guirguis R, et al: Anti-laminin receptor antibody targeting of liposomes with encapsulated doxorubicin to human breast cancer cells in vitro. J Natl Cancer Inst 81:1794-1800, 1989