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Increased expression of the laminin receptor in human colon cancer.
Cioce, V.; Castronovo, Vincenzo; Shmookler, B. M. et al.
1991In Journal of the National Cancer Institute, 83 (1), p. 29-36
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Keywords :
Adenocarcinoma/metabolism/ultrastructure; Adult; Aged; Aged, 80 and over; Blotting, Northern; Colonic Neoplasms/metabolism/ultrastructure; Female; Humans; Immunoenzyme Techniques; Immunohistochemistry; Male; Middle Aged; Neoplasm Staging; Nucleic Acid Hybridization; RNA, Messenger/metabolism; Receptors, Immunologic/genetics/physiology; Receptors, Laminin
Abstract :
[en] It has been proposed that among the various cell-surface proteins capable of interacting with laminin, the 67-kd high-affinity laminin receptor plays a crucial role during tumor invasion and metastasis. In this study, the expression of laminin-receptor-precursor messenger RNA (mRNA) and 67-kd protein was analyzed in human colon adenocarcinoma. In 22 of 23 patients with colon cancer, we found a 2- to 23-fold increase in levels of laminin-receptor-precursor mRNA in the cancer tissues compared with those in matched normal adjacent colonic mucosa. In 10 of 11 cases studied, the level of 67-kd laminin receptor, detected by affinity-purified anti-laminin-receptor synthetic peptide antibodies on immunoblots of matched tumor and normal tissue extracts, was higher in the colon carcinoma tissue. Immunodetection of laminin receptor in tissue sections using anti-laminin-receptor-peptide antibodies confirmed that the increased expression of laminin receptor was specifically associated with the cancer cells. In a series of 72 paraffin sections of colon lesions, we observed a correlation between the expression of the laminin receptor and the Dukes' classification. Our observations indicate that increased expression of laminin-receptor-precursor mRNA is associated with enhanced levels of the 67-kd laminin receptor as well as with the invasive phenotype of colon carcinoma. Detection of this metastasis-associated gene product may be a valuable adjunct in the evaluation of human colon cancer.
Disciplines :
Oncology
Biochemistry, biophysics & molecular biology
Author, co-author :
Cioce, V.
Castronovo, Vincenzo ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie générale et cellulaire - GIGA-R : Labo de recherche sur les métastases
Shmookler, B. M.
Garbisa, S.
Grigioni, W. F.
Liotta, L. A.
Sobel, M. E.
Language :
English
Title :
Increased expression of the laminin receptor in human colon cancer.
Publication date :
1991
Journal title :
Journal of the National Cancer Institute
ISSN :
0027-8874
eISSN :
1460-2105
Publisher :
Oxford University Press, Cary, United States - North Carolina
Volume :
83
Issue :
1
Pages :
29-36
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 May 2010

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