Article (Scientific journals)
Characterization of non pigmented B16 melanoma cell-derived cytotoxic factors.
Siwek, Brigitte; De Pauw-Gillet, Marie-Claire; Quetin-Leclercq, Joëlle et al.
1997In Chemico-Biological Interactions, 103 (1), p. 59-73
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Keywords :
Animals; Cell Division/drug effects; Cell Line/chemistry/drug effects/ultrastructure; Cell Survival/drug effects; Culture Media, Conditioned/chemistry/pharmacology; Cytotoxins/chemistry/pharmacology; DNA/drug effects; Growth Substances/pharmacology; Melanoma, Experimental/metabolism; Mice; Microscopy, Electron; Tumor Cells, Cultured
Abstract :
[en] We analyzed and tried to characterize substance(s) responsible for cytotoxic activities detected in culture media conditioned by non pigmented B16 melanoma cells (NPB16). The different cytological tests used showed that ultrafiltrated conditioned media (CM U1 fraction) contained several cytotoxic factors with a Mw lower than 1000 Da. These factors seemed to act either directly or indirectly on cell membranes, mitochondria, on the cell cycle and on protein and DNA synthesis. A cytotoxic activity could be found even after high dilution of CM U1. These cytotoxic factors were rapidly released by B16 cells in culture, independently of cell confluence. Their activities in the treated cells were also very fast and the cytotoxic effects were irreversible after only a few hours of treatment. These factors were not intermediate products during melanogenesis, neither polyamines, nor proteases. At least one of them seemed to be a small acidic and basic stable peptide without disulfide bounds but not heat stable. The synthesis of at least one of these cytotoxic factors was inhibited by cycloheximide and the cytotoxic activity was partially destroyed by pronase and trypsin, but not by pepsin. The cytotoxicity was not modified by copper complexants or free radical inhibitors (bovine serum albumin (BSA), tyrosine, superoxyde dismutase (SOD), catalase, vitamin E). Furthermore the levels of glutathione peroxydase activity and reduced glutathione did not change after treatment by CM U1 as compared to controls.
Disciplines :
Pharmacy, pharmacology & toxicology
Author, co-author :
Siwek, Brigitte ;  Université de Liège - ULiège
De Pauw-Gillet, Marie-Claire ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie - Cytologie - Département des sciences biomédicales et précliniques
Quetin-Leclercq, Joëlle 
Angenot, Luc  ;  Université de Liège - ULiège > Département de pharmacie > Pharmacognosie
Bassleer, Roger ;  Université de Liège - ULiège > Relations académiques et scientifiques (Médecine)
Language :
English
Title :
Characterization of non pigmented B16 melanoma cell-derived cytotoxic factors.
Publication date :
1997
Journal title :
Chemico-Biological Interactions
ISSN :
0009-2797
eISSN :
1872-7786
Publisher :
Elsevier, Limerick, Ireland
Volume :
103
Issue :
1
Pages :
59-73
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 25 May 2010

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