Article (Scientific journals)
Fourier transform mass spectrometry: A powerful tool for toxin analysis
Quinton, Loïc; Le Caër, Jean-Pierre; Vinh, Joëlle et al.
2006In Toxicon, 47 (6), p. 715-726
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Keywords :
venomics; FT-ICR; de novo
Abstract :
[en] The crude venom of Conus virgo was analyzed by Fourier transform mass spectrometry (FTMS) using both nano-electrospray ionization and MALDI. The analyses were performed directly on the crude venom, without chromatographic separation. The mass fingerprinting of the venom yielded 64 distinct molecular masses in the range 500-4500 Da with two major components at 1328.5142 and 1358.5592 Da. To facilitate the de novo sequencing of these compounds, the disulfide bonds of all components were reduced for the whole venom. The mass accuracy, resolution and sensitivity provided by FTMS were necessary to complete the sequencing of the two new peptides named ViVA and ViVB, that turned out to be conotoxins belonging to the T-superfamily, with the disulfide framework V. The peptides shared 80% similarity and as often observed for this class of compound, they were highly post-translationally modified: amidated C-terminus, pyroglutamic acid residue at the N-terminus and two disulfide bonds. Complementary online nano-LC-nano-ESI-FTMS experiments were undertaken. Among the 130 molecular masses found in the coupling experiments, only 45 were common with those obtained in the direct approach, which means that 21 compounds observed by nano-ESI-FTMS were not detected. This clearly shows that some discriminations against some classes of compounds occur when a chromatographic step is used before mass spectrometry.
Disciplines :
Chemistry
Author, co-author :
Quinton, Loïc  ;  Ecole Polytechnique (France) > Laboratoire des Mécanismes Réactionnels - Palaiseau- France
Le Caër, Jean-Pierre;  Ecole Polytechnique (France) > Laboratoire des Mécanismes Réactionnels - Palaiseau- France
Vinh, Joëlle;  Ecole Supérieure de Physique et de Chimie Industrielles de la ville de Paris > France
Gilles, Nicolas;  Commissariat à l'Energie Atomique (Saclay) - CEA > Département d'ingénierie et d'Etude des Proteines - Gif-sur-Yvette- France
Chamot-Rooke, Julia;  Ecole Polytechnique (France) > Laboratoire des Mécanismes Réactionnels - Palaiseau- France
Language :
English
Title :
Fourier transform mass spectrometry: A powerful tool for toxin analysis
Publication date :
May 2006
Journal title :
Toxicon
ISSN :
0041-0101
eISSN :
1879-3150
Publisher :
Pergamon Press - An Imprint of Elsevier Science, Oxford, United Kingdom
Volume :
47
Issue :
6
Pages :
715-726
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 18 May 2010

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