Reference : Impaired respiration discloses the physiological significance of state transitions in...
Scientific journals : Article
Life sciences : Phytobiology (plant sciences, forestry, mycology...)
Life sciences : Biochemistry, biophysics & molecular biology
Impaired respiration discloses the physiological significance of state transitions in Chlamydomonas.
[fr] Une respiration déficiente révèle la signification physiologique de transitions d'état chez Chlamydomonas.
Cardol, Pierre mailto [Université de Liège - ULiège > Département des sciences de la vie > Génétique >]
Alric, Jean [> > > >]
Girard-Bascou, Jacqueline [> > > >]
Franck, Fabrice mailto [Université de Liège - ULiège > > Biochimie végétale >]
Wollman, Francis*-Andre [> > > >]
Finazzi, Giovanni [> > > >]
Proceedings of the National Academy of Sciences of the United States of America
National Academy of Sciences
Yes (verified by ORBi)
[en] Adenosine Triphosphate/metabolism ; Animals ; Chlamydomonas reinhardtii/genetics/growth & development/physiology ; Electron Transport ; Genes, Protozoan ; Light ; Mitochondria/metabolism ; Mutation ; Oxidation-Reduction ; Photosynthesis/genetics/physiology ; Plastoquinone/metabolism
[en] State transitions correspond to a major regulation process for photosynthesis, whereby chlorophyll protein complexes responsible for light harvesting migrate between photosystem II and photosystem I in response to changes in the redox poise of the intersystem electron carriers. Here we disclose their physiological significance in Chlamydomonas reinhardtii using a genetic approach. Using single and double mutants defective for state transitions and/or mitochondrial respiration, we show that photosynthetic growth, and therefore biomass production, critically depends on state transitions in respiratory-defective conditions. When extra ATP cannot be provided by respiration, enhanced photosystem I turnover elicited by transition to state 2 is required for photosynthetic activity. Concomitant impairment of state transitions and respiration decreases the overall yield of photosynthesis, ultimately leading to reduced fitness. We thus provide experimental evidence that the combined energetic contributions of state transitions and respiration are required for efficient carbon assimilation in this alga.

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