Reference : Direct Quantitative Analysis of Linamarin in Cassava by High-Performance Thin Layer C...
Scientific journals : Article
Human health sciences : Pharmacy, pharmacology & toxicology
Direct Quantitative Analysis of Linamarin in Cassava by High-Performance Thin Layer Chromatography
Bodart, Patricia [Université de Liège - ULiège > Département de pharmacie > Pharmacognosie >]
Penelle, Jacques [Université de Liège - ULiège > Services généraux (Faculté de médecine) > Relations académiques et scientifiques (Médecine) >]
Angenot, Luc mailto [> > > >]
Noirfalise, Alfred mailto [> > > >]
Journal of Planar Chromatography- Modern TLC
[en] HPTLC ; Denistometry ; Linamarin ; Cyanogenic glycosides ; Manihot esculenta
[en] A simple, rapid and accurate HPTLC quantitative procedure is presented for the direct determination of linamarin. A cassava sample was extracted with boiling 80% v/v methanol and the extract and linamarin were applied to silicagel HPTLC plates prewashed by development with methanol.The plates were first developed to a distance of 30mm with ethyl acetate-acetone-water, 40+50+10 (v/v), then to a distance of 85 mm with ethyl acetate-formic acid-water, 60+10+10 (v/v). the spots were visualized by dipping the plate into a solution af aniline (2%), and phosphoric acid 15% in acetone, then heating at 105°C for 60 min. Densitometric quantification was effected at lambda= 525 nm by transmission scanning. The method was validated for accuracy, intraday, and interday reproducibility of peak area, linearity, and detection limit.

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Journal of Planar Chromatography vol 11 _ 1998 pp 38_42.pdfNo commentaryPublisher postprint222.45 kBRequest copy

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