Conformationally driven gas-phase H/D exchange of dinucleotide negative ions

[en] Gas-phase hydrogen/deuterium exchange of six deprotonated dinucleoticles with CD3OD was performed in the second hexapole of a Fourier transform ion-cyclotron resonance (FTICR) mass spectrometer. To complete these experiments, dynamic simulations were carried out to investigate the different conformations adopted by the dinucleotides. In the experimental conditions and in integrating the experimental and theoretical results, H/D exchange was shown to be controlled by hydrogen accessibility and not by the chemical nature of the heteroatom bearing the exchangeable hydrogen. A model including simultaneous H/D exchanges at the experimental time scale was used to reproduce the dinucleotide H/D exchange kinetic plots. The relay mechanism was not relevant for dinucleotides. This allowed the H/D exchange rates to be directly linked to conformations.

Disciplines :

Physics
Chemistry

Author, co-author :

Balbeur, Dorothée ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse

Dehareng, Dominique ; Université de Liège - ULiège > Centre d'ingénierie des protéines

De Pauw, Edwin ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse

Language :

English

Title :

Conformationally driven gas-phase H/D exchange of dinucleotide negative ions

Publication date :

October 2007

Journal title :

Journal of the American Society for Mass Spectrometry

ISSN :

1044-0305

eISSN :

1879-1123

Publisher :

Elsevier Science Inc, New York, United States - New York

Volume :

Issue :

Pages :

1827-1834

Peer reviewed :

Peer Reviewed verified by ORBi

Funders :

BELSPO - SPP Politique scientifique - Service Public Fédéral de Programmation Politique scientifique
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]

Available on ORBi :

since 05 January 2009

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