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Poster (Scientific congresses and symposiums)
Role of coactivators SRC-1 and CARM1 in estrogen receptor-alpha and beta-dependent cell proliferation in the dentate gyrus of adult female rats
Charlier, Thierry; Lieblich, Stephanie E; Pawluski, Jodi et al.
200737th Annual Meeting of the Society for Neuroscience
 

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Abstract :
[en] Nuclear receptors such as the estrogen receptors (ER) require the presence of coactivator proteins, such as the steroid receptor coactivator (SRC-1) and coactivator-associated arginine methyltransferase (CARM1) to enhance the transcription of target genes. Importantly, in vitro work suggests that ER􀀁 and ER􀀂 differ in the ability to recruit coactivators such as SRC-1. For example, SRC-1 has a strong affinity for ER􀀁 and a weaker affinity for ER􀀂. Interestingly, both ER􀀁 and ER􀀂 are individually involved in estradiol-enhanced cell proliferation in the dentate gyrus of adult female rats. In addition, previous work suggests a role for CARM1 in cell proliferation and for SRC-1 in cell differentiation, therefore the present study aimed to determine whether proliferating cells in the dentate gyrus of the hippocampus co-express the coactivators SRC-1 and CARM1. We also aimed to determine whether ER􀀁 and ER􀀂 agonists would result in altered expression of SRC-1 and CARM1 in new proliferating cells in the dentate gyrus. To investigate this, adult female rats were ovariectomized and treated with either the ER􀀁 agonist Propyl-pyrazole triol (PPT), the ER􀀂 agonist diarylpropionitrile (DPN), estradiol benzoate (EB), or vehicle (CTRL). Rats were then injected with BrdU (200 mg/kg) and sacrificed 24 hours later. Preliminary data suggests that DPN, PPT and EB increase cell proliferation in the dentate gyrus compared to the vehicle-injected group. Interestingly, the number of proliferating cell expressing SRC-1 is similar in all groups, suggesting that neither of the ER agonists nor EB treatment affects the co-expression of BrdU+ cells with SRC-1. However, additional measurements are currently being done to investigate whether CARM-1 is differentially expressed in proliferating cells in the hippocampus following selective ER agonist treatment.
Disciplines :
Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Charlier, Thierry ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau
Lieblich, Stephanie E;  University of British Columbia > Department of Psychology
Pawluski, Jodi ;  University of British Columbia > Department of Psychology
Galea, Liisa, AM;  University of British Columbia > Department of Psychology
Language :
English
Title :
Role of coactivators SRC-1 and CARM1 in estrogen receptor-alpha and beta-dependent cell proliferation in the dentate gyrus of adult female rats
Publication date :
2007
Event name :
37th Annual Meeting of the Society for Neuroscience
Event place :
San Diego, United States
Audience :
International
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since 08 January 2010

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