Aggression/physiology; Analysis of Variance; Animals; Corticosterone/blood/metabolism; Iodine Radioisotopes; Male; Plasma/metabolism; Progesterone/blood/metabolism; Radioimmunoassay; Random Allocation; Seasons; Sexual Behavior, Animal/physiology; Sparrows/blood/physiology; Territoriality; Testosterone/blood/metabolism; Time Factors; Transcortin/metabolism; Tritium
Abstract :
[en] In birds, corticosteroid-binding globulin (CBG) binds corticosterone, progesterone and testosterone. The concentration of each ligand can alter the binding of the other ligands through competitive interactions. Thus, an increase in corticosterone or progesterone may displace testosterone bound to CBG, leading to an increase in bioactive free testosterone levels without affecting total testosterone levels in the circulation. Aggressive interactions increase plasma total testosterone levels in some birds but not in others. Here, we tested the hypothesis that aggressive encounters in the late breeding season would not increase total testosterone levels in plasma, but would alter CBG, total corticosterone or total progesterone levels in such a way as to modify the number of available binding sites and therefore occupancy by testosterone. A marked decrease in CBG occupancy by testosterone would indirectly suggest an increase in free testosterone levels in plasma. Wild male white-crowned sparrows were exposed to a simulated territorial intrusion (STI) or control for 30 min. Subjects were then caught and bled. We measured CBG using a ligand-binding assay and corticosterone, progesterone and testosterone using highly sensitive radioimmunoassays. STI significantly increased aggressive behaviors but did not affect plasma total testosterone levels. STI significantly increased plasma CBG and total corticosterone levels and decreased plasma total progesterone levels. We predict that CBG occupancy by corticosterone will increase slightly following an aggressive encounter. However, this small change is unlikely to increase free testosterone levels, because of the large number of seemingly unoccupied CBG binding sites in these subjects.
Disciplines :
Zoology
Author, co-author :
Charlier, Thierry ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Biologie de la différenciation sexuelle du cerveau
Underhill, Caroline; University of British Columbia - UBC > Department of Obstetrics and Gynaecology and Child and Family Research Institute
Hammond, Geoffrey L.; University of British Columbia - UBC > Department of Obstetrics and Gynaecology and Child and Family Research Institute
Soma, Kiran K.; University of British Columbia - UBC > Department of Psychology / Department of Zoology
Language :
English
Title :
Effects of aggressive encounters on plasma corticosteroid-binding globulin and its ligands in white-crowned sparrows.
Publication date :
2009
Journal title :
Hormones and Behavior
ISSN :
0018-506X
eISSN :
1095-6867
Publisher :
Academic Press, San Diego, United States - California
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