Expression At The Cell-Surface Of Native Fusion Protein Of The Newcastle-Disease Virus (Ndv) Strain Italien From Cloned Cdna

Espion, D.; Dehenau, S.; Letellier, C.; Wemers, Cd.; Brasseur, Robert; Young, Jf.; Gross, M.; Rosenberg, M.; Meulemans, G.; Burny, Arsène

doi:10.1007/BF01311336

Article (Scientific journals)

Expression At The Cell-Surface Of Native Fusion Protein Of The Newcastle-Disease Virus (Ndv) Strain Italien From Cloned Cdna

Espion, D.; Dehenau, S.; Letellier, C. et al.

1987 • In Archives of Virology, 95 (1-2), p. 79-95

Peer Reviewed verified by ORBi

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https://hdl.handle.net/2268/34893

DOI
10.1007/BF01311336

PubMed
3592986

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Abstract :

[en] A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long open reading frame encodes for a protein of 553 amino acids, with a calculated molecular weight of 59,153, consisting of twelve cysteine residues and six potential glycosylation sites. The protein sequence contains a hydrophobic region at the N-terminus of F1 and a presumptive long transmembrane fragment near the C-terminus. Comparison of the F proteins from NDV strains Italien and Australia-Victoria shows that the sequences are very similar, with conservation of most cysteine residues and of the potential glycosylation sites. The F coding sequence was inserted into the genome of vaccinia virus under the control of vaccinia P7.5 transcriptional regulatory sequences. Expression of F protein was demonstrated by indirect immunofluorescence with five anti-F monoclonal antibodies known to react with conformational epitopes.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Espion, D.

Dehenau, S.

Letellier, C.

Wemers, Cd.

Brasseur, Robert ; Université Libre de Bruxelles - ULB > Laboratoire Chimie-Physique des Macromolecules aux Interfaces

Young, Jf.

Gross, M.

Rosenberg, M.

Meulemans, G.

Burny, Arsène ; Université de Liège - ULiège > Agro Biotech Gembloux

Language :

English

Title :

Expression At The Cell-Surface Of Native Fusion Protein Of The Newcastle-Disease Virus (Ndv) Strain Italien From Cloned Cdna

Publication date :

1987

Journal title :

Archives of Virology

ISSN :

0304-8608

eISSN :

1432-8798

Publisher :

Springer, Germany

Volume :

Issue :

1-2

Pages :

79-95
79-95

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.ncbi.nlm.nih.gov/pubmed/3592986

Available on ORBi :

since 23 February 2010

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