[en] Respiratory syncytial virus (RSV) is a prominent cause of airway morbidity in children under 1 yr of age. It is assumed that host factors influence the severity of the disease presentation and thus the need for hospitalization. As a first step toward the identification of the underlying genes involved, this study was undertaken to establish whether inbred mouse strains differ in susceptibility to pneumonia virus of mice (PVM), the murine counterpart of RSV, which has been shown to accurately mimic the RSV disease of children. With this purpose in mind, double-chamber plethysmography and carbon monoxide uptake data were collected daily for 7 days after inoculation of PVM in six inbred strains of mice. In parallel, histological examinations and lung viral titration were carried out from day 5 to day 7 after inoculation. Pulmonary structure/function values reflected the success of viral replication in the lungs and revealed a pattern of continuous variation, with resistant, intermediate, and susceptible strains. The results suggest that SJL (resistant) and 129/Sv (susceptible) strains should be used in crossing experiments aimed at identifying genes controlling pneumovirus replication by the positional cloning approach. Similarly, crossing experiments using BALB/c or C57BL/6 (resistant) and DBA/2 or 129/Sv (susceptible) will allow the identification of the genes involved in the control of pulmonary inflammation during pneumovirus infection.
Disciplines :
Immunology & infectious disease
Author, co-author :
Bui Tran Anh, Dao; Université de Hanoi > Médecine vétérinaire > Pathologie
Faisca, Rui-Pedro; Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Desmecht, Daniel ; Université de Liège - ULiège > Morphologie et Pathologie > Pathologie spéciale et autopsies
Language :
English
Title :
Differential resistance/susceptibility patterns to pneumovirus infection among inbred mouse strains
Publication date :
2006
Journal title :
American Journal of Physiology - Lung Cellular and Molecular Physiology
ISSN :
1040-0605
eISSN :
1522-1504
Publisher :
American Physiological Society, Bethesda, United States - Maryland
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