[en] Ponds are a large source of atmospheric methane (CH4), a potent greenhouse gas, resulting from the net balance between input from sedimentary methanogenesis and removal by CH4 oxidation (MOX). Here, we test whether methanogenesis pathways (acetoclastic or hydrogenotrophic) and MOX might differ between clear-water (macrophyte-dominated) and turbid-water (phytoplankton-dominated) ponds. We measured the 13C/12C ratio of CH4 (δ13C-CH4) from gas trapped in bubble traps, from bubbles deliberately released by the perturbing sediments, and in dissolved CH4 in the water column in four urban ponds in Brussels, Belgium (Leybeek, Pêcheries, Tenreuken, Silex). In summer, the δ13C-CH4 values of sediment bubbles indicated that the hydrogenotrophic methanogenesis pathway appeared to be more important in clear-water (macrophyte-dominated) ponds (Leybeek and Pêcheries), whereas the acetoclastic methanogenesis pathway appeared to be more important in turbid-water (phytoplankton-dominated) ponds (Tenreuken and Silex). The δ13C-CH4 values from bubble traps indicated a seasonal shift from acetoclastic methanogenesis pathway in spring–summer to hydrogenotrophic methanogenesis in fall. The δ13C-CH4 of dissolved CH4 indicated higher rates of MOX in turbid-water ponds (Leybeek and Pêcheries) compared to clear-water ponds (Tenreuken and Silex), with an overall positive relation with total suspended matter and chlorophyll-a concentrations. The presence of suspended particles likely enhanced MOX by reducing light inhibition of MOX and/or by serving as substrates in the water column for attached methanotrophic bacteria. MOX represented ~ 80% of the total dissolved CH4 removal from the water column (MOX plus diffusive emission to the atmosphere) in the turbid-water ponds (Leybeek and Pêcheries) and < 60% in the clear-water ponds (Tenreuken and Silex). Our results suggest that shifts from clear- to turbid-water ecological states due to eutrophication may change CH4 production pathways (with a higher contribution of acetoclastic versus hydrogenotrophic methanogenesis) and enhance dissolved CH4 removal by MOX in the water column.
Research Center/Unit :
FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège
Disciplines :
Aquatic sciences & oceanology
Author, co-author :
Borges, Alberto ; Université de Liège - ULiège > Département d'astrophysique, géophysique et océanographie (AGO) > Chemical Oceanography Unit (COU)
Gypens, Nathalie; Ecology of Aquatic Systems, Université Libre de Bruxelles, Brussels, Belgium
Bauduin, Thomas; Chemical Oceanography Unit, University of Liège, Liège, Belgium ; Ecology of Aquatic Systems, Université Libre de Bruxelles, Brussels, Belgium
Language :
English
Title :
Methanogenesis pathways and methane oxidation in two clear-water and two turbid-water urban ponds in Brussels (Belgium)
Publication date :
April 2026
Journal title :
Aquatic Sciences
ISSN :
1015-1621
eISSN :
1420-9055
Publisher :
Springer Science and Business Media Deutschland GmbH
F.R.S.-FNRS - Fonds de la Recherche Scientifique Innoviris Brussels FRIA - Fonds pour la Formation à la Recherche dans l'Industrie et dans l'Agriculture
Funding text :
TB received funding from the Institute for the Encouragement of Scientific Research and Innovation (Innoviris) of the Brussels-Capital Region as part of the Smartwater project (RBC/2020-EPF-6\u00A0h) and from the Fonds pour la formation \u00E0 la Recherche dans l\u2019Industrie et dans l\u2019Agriculture\u201D (FRIA, Belgium). The Picarro G2201-i isotopic analyzer was funded by the Fund for Scientific Research (FRS-FNRS) (U.N005.21). AVB is a research director at FRS-FNRS.TB received funding from the Institute for the Encouragement of Scientific Research and Innovation (Innoviris) of the Brussels-Capital Region as part of the Smartwater project (RBC/2020-EPF-6 h) and from the Fonds pour la formation \u00E0 la Recherche dans l\u2019Industrie et dans l\u2019Agriculture\u201D (FRIA, Belgium). The Picarro G2201-i isotopic analyzer was funded by the Fund for Scientific Research (FRS-FNRS) (U.N005.21). AVB is a research director at FRS-FNRS.
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